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High flux detection method and biochip based on nucleic acid address coding

An address coding and detection method technology, which is applied in the field of high-throughput detection methods and biochips based on nucleic acid address coding, can solve the problems of lack of protein detection method biochips and limit the progress of proteomics research, and achieve reliable detection results. Improve detection types and good stability

Inactive Publication Date: 2013-12-11
广东朗源生物科技有限公司
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Problems solved by technology

[0007] At this stage, there is a lack of an efficient, high-throughput protein detection method and corresponding biochip, which greatly limits the progress of proteomics research.

Method used

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  • High flux detection method and biochip based on nucleic acid address coding

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Embodiment Construction

[0029] A high-throughput detection method based on nucleic acid address coding, comprising the following steps:

[0030] 1) immobilizing the first single-stranded nucleic acid array on a solid phase carrier;

[0031] 2) coupling a detection substance capable of specifically reacting with the analyte to a second single-stranded nucleic acid to obtain a detection body, wherein the second single-stranded nucleic acid is complementary to the first single-stranded nucleic acid;

[0032] 3) Mix and react the analyte with the analyte, then add the labeled secondary antibody, and continue the reaction to obtain the analyte-analyte-secondary antibody complex; combine the above complex with the first single-chain immobilized on the solid phase carrier For nucleic acid hybridization, wash to remove unhybridized reactants; or mix and react the test substance with the test substance, hybridize with the first single-stranded nucleic acid immobilized on the solid phase carrier, and wash; the...

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Abstract

The invention discloses a high flux detection method and detection biochip based on nucleic acid address coding. According to the invention, the principle of specific complementation and hybridization of nucleic acid is ingeniously utilized to locate nucleic acid with different sequences at different areas of the chip; conjugates of complementary nucleic acid and proteins are located at corresponding positions on the biochip because of interaction among nucleic acid; therefore, disadvantages and deficiency of conventional protein chips are overcome, a whole reaction is carried out in a liquid environment, and since genetic coding is infinite, limitation of bead coding in liquid phase chips is overcome. Thus, the detection biochip provided in the invention combines the advantages of solid phase chips and liquid phase chips and enables a more accurate high flux detection reaction to be achieved.

Description

technical field [0001] The invention relates to a high-throughput detection method and a corresponding biological detection chip, in particular to a high-throughput detection method and a biological chip based on nucleic acid address coding. Background technique [0002] Biochip technology is a type of biological analysis and detection technology developed with the Human Genome Project in the mid-1990s. It has been widely used in gene sequencing, gene expression analysis, gene mutation and polymorphism detection, and the detection of microorganisms in food. , clinical medicine, etc., and has become one of the research hotspots in the scientific community (Lu Weihong, Fu Li, Zheng Qi, etc. Application and prospect of biochip technology [J]. Sensors and Microsystems, 2006, 25(6): 1-4). [0003] Biochips are divided into gene chips, protein chips, cell chips, and tissue chips according to the biological components detected and analyzed (Meilin, Song Shiyuan, Zha Zhongyong, etc...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/543C12Q1/68
Inventor 龚桂香
Owner 广东朗源生物科技有限公司
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