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Method for quantitatively determining phenolic acid compounds in barley grains

A technology of quantitative determination and determination method, which is applied in the field of agriculture, can solve problems such as complex analysis conditions, unfavorable sample determination, and increased analysis cost, and achieve the effect of accurate analysis method, high sensitivity and good repeatability

Inactive Publication Date: 2012-04-25
SHIHEZI UNIVERSITY
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  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the phenolic compounds in barley grains analyzed by HPLC in the past are less. When there are many components to be analyzed, the analysis conditions are often complicated and the analysis time is long, which greatly increases the analysis cost and is not conducive to large-scale analysis. Determination of batch samples

Method used

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  • Method for quantitatively determining phenolic acid compounds in barley grains
  • Method for quantitatively determining phenolic acid compounds in barley grains
  • Method for quantitatively determining phenolic acid compounds in barley grains

Examples

Experimental program
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Embodiment 1

[0037] 1 material

[0038] 1.1 Instrument

[0039] Agilent LC1200 high performance liquid chromatograph: Including Agilent 1200 quaternary pump with online degasser, 1200 series automatic sample management system, intelligent column oven, 1200 programmable variable wavelength UV detector and Agilent ChemStation B.04.01 chromatogram Data processing system; SUPELCOAscentis ?? C18 chromatographic column (150mm×4.6mm, 5μm).

[0040] 1.2 Reagents and samples

[0041] Anhydrous methanol and anhydrous formic acid are Fisher's products; ultra-pure water is prepared by a laboratory-specific ultra-pure water machine (Taiwan Eco-Chengdu Kangning Experimental Pure Water Equipment Factory). Gallic acid, protocatechuic acid, chlorogenic acid, p-hydroxybenzoic acid, caffeic acid, vanillic acid, syringic acid, meta-hydroxybenzoic acid, p-coumaric acid, ferulic acid, veratric acid, o-coumaric acid and water 13 standard phenolic acids including salicylic acid were purchased from Sigma, with a purity ...

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Abstract

The invention discloses a method for quantitatively determining phenolic acid compounds in barley grains. The method comprises the following steps of: aiming at the physicochemical properties of phenolic acid, extracting the phenolic acid compounds from the barley grains by using a methanol solution at a certain concentration; performing centrifugation on extract; concentrating and filtering the extract; loading the prepared sample extract; and eluting and separating the prepared sample extract by using a methanol-methane acid aqueous solution. The analytical method is accurate, simple and convenient, has high sensitivity, is high in selectivity and repetitiveness; by the method, the types of the phenolic acid compounds in the barley grains can be comprehensively tested at one time; and compared with the conventional method, the method has the advantage that: analysis time is greatly shortened, so that basic requirements for quickly determining and evaluating the phenolic acid compounds in the barley grains on a large scale during production can be met; and the method is suitable for grains of any barley variety.

Description

Technical field [0001] The invention belongs to the field of agriculture, and particularly relates to a quantitative determination method of phenolic acid compounds in barley grains. Background technique [0002] Phenolic acid refers to phenols with a certain carboxylic acid function. It is the second largest secondary metabolite in plants after flavonoids, and it is also the main bound phenol in grains. Phenolic acid is a good antioxidant, widely used in the fields of medicine and health care. It has a variety of biological activities such as prevention of cardiovascular disease, anti-cancer, antibacterial, anti-inflammatory, anti-mutation and anti-AIDS virus. [0003] Barley is a crop that is widely planted around the world with low price. It is currently mainly used as a raw material for the feed industry and brewing beer. The content of phenolic acid compounds in barley grains is relatively high and rich in types. The main components are gallic acid, protocatechuic acid, p-hyd...

Claims

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Application Information

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IPC IPC(8): G01N30/89
Inventor 王祥军齐军仓
Owner SHIHEZI UNIVERSITY
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