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Champignon beta-glucan having anti-inflammation activity, and preparation method and application thereof

A dextran and anti-inflammatory technology, applied in the fields of polymer physics and biology, can solve the problem of less research on lentinan, achieve the effect of no toxic side effects, simple extraction process, and low cost

Inactive Publication Date: 2012-04-11
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are relatively few further studies on lentinan, especially the discovery and research of new polysaccharides

Method used

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  • Champignon beta-glucan having anti-inflammation activity, and preparation method and application thereof
  • Champignon beta-glucan having anti-inflammation activity, and preparation method and application thereof
  • Champignon beta-glucan having anti-inflammation activity, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Purchase commercially available shiitake mushroom fruiting bodies produced in Suizhou, Hubei, take 200 g of shiitake mushroom fruiting bodies, crush them to 5 mesh, and perform Soxhlet extraction with 600 ml of ethyl acetate and 600 ml of acetone for 8 hours each. Then, it was stirred overnight at room temperature with 0.9% NaCl aqueous solution, centrifuged to collect the residue, and repeated three times. The residue was leached three times with 4 times the volume of hot water (100°C), centrifuged for half an hour each time, and the residue was collected; the residue was subjected to ultrasonic extraction (25°C, 20KHz, 600W, 16min) in pure water to obtain the extract; Clear the extract with H 2 o 2 Decolorization, after 6 times of Sevag method to remove free protein, dialyze with water and twice distilled water respectively (cutoff M w =8,000), concentrated and freeze-dried to obtain Lentinus edodes β-glucan (LNT-S). Use NMR, IR, GC-MS, etc. to prove that LNT-S is ...

Embodiment 2

[0032] Purchase commercially available shiitake mushroom fruiting bodies produced in Suizhou, Hubei, take 200 g of shiitake mushroom fruiting bodies, crush them to 3 meshes, and perform Soxhlet extraction with 500 ml of ethyl acetate and 500 ml of acetone for 6 hours each. Then stir with physiological saline at room temperature for 8 hours, centrifuge, collect the residue, repeat 4 times. The residue was leached 4 times with 5 times the amount of hot water (120°C), 20 minutes each time, and centrifuged to collect the residue; the residue was subjected to ultrasonic extraction (30°C, 18KHz, 500W, 20min) in pure water to obtain the extract; Clear the extract with H 2 o 2 After decolorization, after 5 times of Sevag method to remove free protein, dialyze with water and twice distilled water respectively (cutoff M w =8,000), concentrated and freeze-dried to obtain Lentinus edodes β-glucan (LNT-S). It was identified that it was identical to the Lentinus edodes β-glucan (LNT-S) p...

Embodiment 3

[0034] Purchase commercially available shiitake mushroom fruiting bodies produced in Suizhou, Hubei, take 200 g of shiitake mushroom fruiting bodies, crush them to 3 mesh, and perform Soxhlet extraction with 500 ml of ethyl acetate and 500 ml of acetone for 10 hours each. Then stir with physiological saline at room temperature for 24 hours, centrifuge, collect the residue, and repeat twice. The residue was leached twice with 5 times the amount of hot water (120°C) for 40 minutes each time, and centrifuged to collect the residue; the residue was subjected to ultrasonic extraction (20°C, 30KHz, 500W, 18min) in pure water to obtain the extract; Clear the extract with H 2 o 2 Decolorization, after 10 times of Sevag method to remove free protein, dialyzed with water and twice distilled water respectively (cutoff M w =8,000), concentrated and freeze-dried to obtain Lentinus edodes β-glucan (LNT-S). It was identified that it was identical to the Lentinus edodes β-glucan (LNT-S) pr...

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Abstract

The invention discloses a champignon beta-glucan. The main chain of the champignon beta-glucan is beta-(1->3)-D-glucan; on average, every 7-10 glucose residues on the main chain contain 2-3 (1->6) bonded glucose lateral groups; and the champignon beta-glucan is in the shape of a rod-like chain in water. The preparation method comprises the following steps: crushing the fruit body of champignon, degreasing, soaking in normal saline, centrifuging, and collecting leavings; extracting the leavings in boiling water at 100-130 DEG C, centrifuging, collecting leavings, carrying out ultrasonic extraction in pure water, and centrifuging to obtain an extracting solution; and decoloring the extracting solution, removing free proteins, dialyzing with water, concentrating, and carrying out freeze-drying to obtain the champignon beta-glucan. The champignon beta-glucan has an obvious effect of activating macrophages and a superior effect of inhibiting the release of inflammation regulation factors NO and TNF-alpha, and can be used for the preparation of medicaments or health products for improving immune function and resisting inflammation.

Description

technical field [0001] The invention relates to a rod-shaped shiitake mushroom β-glucan with anti-inflammatory activity and a preparation method thereof. It belongs to the field of polymer physics, and it also belongs to the field of biology. Background technique [0002] Inflammation is a host response to intrinsic or extrinsic environmental stimuli. This response process is strictly controlled by physiological conditions. Acute inflammation in a short period of time has a therapeutic effect. However, when the inflammation lasts too long or turns into chronic inflammation due to physiological loss of control, it will have a harmful effect on the body and even cause some serious damage. Diseases appear, such as cardiovascular and cerebrovascular diseases, diabetes, arthritis, Alzheimer's disease, autoimmune diseases and cancer. In recent years, many researchers have devoted themselves to the research and development of anti-inflammatory drugs. Among many anti-inflammatory...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02A61K31/716A61P29/00A61P37/04A23L1/09
Inventor 许小娟陈攀贾学伟张俐娜
Owner WUHAN UNIV
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