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Preparation method of chicken infectious rhinitis lipid inactivated vaccine

A chicken infectious rhinitis and inactivated vaccine technology, applied in the field of preparation of veterinary biological products, to achieve the effects of saving production costs, increasing egg production rate, and reducing dosage

Inactive Publication Date: 2012-04-11
SHANDONG BINZHOU BOLAIWEI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a preparation method of a chicken infectious rhinitis lipid inactivated vaccine aimed at the defects of the existing inactivated vaccines. The vaccine prepared by the present invention is easy to absorb, has no toxic and side effects, and has no immune generation time. Short, long duration of immunity, good immune effect, can effectively prevent and control chicken infectious rhinitis, and low manufacturing cost

Method used

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  • Preparation method of chicken infectious rhinitis lipid inactivated vaccine
  • Preparation method of chicken infectious rhinitis lipid inactivated vaccine

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1, the preparation of chicken infectious rhinitis lipid inactivated vaccine, carries out according to the following steps:

[0027] (1) The preparation of type A Haemophilus paragallinarum inactivated antigen bacterium liquid is carried out in the following order:

[0028] Preparation of primary strains: Streak inoculation of Haemophilus paragallinarum type A strains on chicken broth agar plates, in CO 2 The volume content is 5%, the temperature is 16h in the candle jar of 37 ℃, select the 3 typical colonies of 1mm in diameter that meet the characteristics of the strain, dilute it with 10ml sterilized chicken broth and inoculate it into the yolk sac of 7-day-old SPF chicken embryo. Inoculate 0.2ml per embryo, continue to hatch at 37°C, collect the yolk fluid of dead embryos within 30 hours, and after pure inspection confirms that there is no miscellaneous bacteria, it will be used as a first-class strain and stored below -20°C for no more than 30 days ;

[...

Embodiment 2

[0040] Embodiment 2, the preparation of chicken infectious rhinitis lipid inactivated vaccine, carries out according to the following steps:

[0041] (1) The preparation of type A Haemophilus paragallinarum inactivated antigen bacterium liquid is carried out in the following order:

[0042] Preparation of primary strains: Streak inoculation of Haemophilus paragallinarum type A strains on chicken broth agar plates, in CO 2 The volume content is 5%, and the temperature is 17h in the candle jar of 37 ℃, selects 5 typical colonies with a diameter of 1mm that meet the characteristics of the strain, dilutes it with 10ml sterilized chicken broth, and inoculates it into the yolk sac of 6-day-old SPF chicken embryos. Embryos are inoculated with 0.2ml, continue to hatch at 37°C, collect the yolk fluid of dead embryos within 30 hours, and after pure inspection confirms that there is no miscellaneous bacteria, it will be used as a first-class strain and stored below -20°C for no more than...

Embodiment 3

[0049] Embodiment 3, the preparation of chicken infectious rhinitis lipid inactivated vaccine, carries out according to the following steps:

[0050] (1) The preparation of type A Haemophilus paragallinarum inactivated antigen bacterium liquid is carried out in the following order:

[0051] Preparation of primary strains: Streak inoculation of Haemophilus paragallinarum type A strains on chicken broth agar plates, in CO 2 The volume content is 5% and the temperature is 37 ° C for 18 hours. Select 3 typical colonies with a diameter of 1 mm that meet the characteristics of the strain and dilute them with 10 ml of sterilized chicken broth and inoculate them into the yolk sac of 7-day-old SPF chicken embryos. Each embryo is inoculated 0.2ml, continue to incubate at 37°C, collect the yolk fluid of dead embryos within 30 hours, and after pure inspection confirms that there are no bacteria, it will be used as a first-class strain and stored below -20°C for no more than 30 days;

[0...

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Abstract

The invention provides a preparation method of a chicken infectious rhinitis lipid inactivated vaccine, belonging to the technical field of biological products for animals. The preparation method is implemented by the following steps of: preparing a type A haemophilus paragallinarum inactivated antigen bacterial liquid and a type C haemophilus paragallinarum inactivated antigen bacterial liquid; uniformly mixing the type A haemophilus paragallinarum inactivated antigen bacterial liquid with the type C haemophilus paragallinarum inactivated antigen bacterial liquid in the volume ratio of 1:1, and pouring into an emulsifying tank; stirring with a mixer; slowly adding poplar bark lipid at a low speed till the final volume percentage concentration of the poplar bark lipid in the mixture is 2.0-3.8 percent; and continually stirring and emulsifying for 30-60 minutes to obtain the vaccine, wherein the final bacterium content of a type A strain and the final bacterium content of a type C strain in the vaccine are 1,000,000,000 cfu / ml. The vaccine has the advantages of easiness for absorbing, no toxic or side effect, short immunogenic time, long immune duration, good immune effect and capability of effectively preventing chicken infectious rhinitis and mycoplasma gallisepticum.

Description

technical field [0001] The invention relates to the field of preparation of veterinary biological products, in particular to a method for preparing an inactivated lipid vaccine for chicken infectious rhinitis. Background technique [0002] Chicken infectious rhinitis is an acute upper respiratory infectious disease caused by Haemophilus paragallinarum. The main features are facial swelling, serous or mucous secretions in the nasal cavity, and conjunctivitis. Stagnation and a significant drop in laying hen egg production (10-40% reduction in egg production), most commonly occurring in chickens and laying hens aged 8-12 weeks. The disease is distributed all over the world, which hinders the growth and development of bred chickens and increases the elimination rate, and the egg production of laying hens is significantly reduced, causing huge economic losses to the chicken industry. The disease can be treated with sensitive drugs, but it is very easy to produce drug resistance,...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K39/102A61P31/04A61P11/02A61P27/02
Inventor 杨灵芝姚奋卯孟照洁王娟郝圣峰王秀云
Owner SHANDONG BINZHOU BOLAIWEI BIOTECH
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