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Method for improving adventitious bud differentiation rate of tartary buck wheat through suspension culture

A technique of buckwheat tartar and suspension culture is applied in the field of plant tissue culture to achieve the effects of promoting callus growth, improving the differentiation rate of adventitious buds and inhibiting browning

Inactive Publication Date: 2012-10-10
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there have been a few reports on the tissue culture of Tartary buckwheat (F.tartaricum (L.) Gaerth). Regenerated plants (Journal of Northwest Botany, 2006; Journal of Applied and Environmental Biology, 2009), but the research on promoting the differentiation of adventitious buds of tartary buckwheat through suspension culture of callus has not been reported at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Obtaining of explants

[0026] Soak the mature Tartary buckwheat seeds in water for 10 minutes and then peel them off, sterilize the peeled seeds in 70% alcohol for 1 minute, and then sterilize them in 0.1% mercuric chloride for 15 minutes. Finally rinse four times with sterile water.

[0027] The sterilized seeds were inoculated on 1 / 2MS (Murashige-Skoog) solid medium without sucrose, and germinated at a temperature of 25°C±2°C and a light intensity of 2000Lx.

[0028] (2) Induction of callus

[0029] Cut the hypocotyls of germinated 7-day-old tartary buckwheat aseptic seedlings into 0.5 cm long segments, and inoculate them on the callus-inducing medium. The composition of the callus-inducing medium is: add Agar with a mass concentration of 0.8%, sucrose with a mass concentration of 3%, 2,4-D at 2mg / L, 6-BA at 0.5mg / L, AgNO at 10mg / L 3 ; Cultivate for 15 days at a temperature of 25°C±2°C, 500Lx. Both ends of the hypocotyl expand rapidly, and the callus is induc...

Embodiment 2

[0036] (1) Obtaining of explants

[0037] Soak the ripe tartar buckwheat seeds in water for 10 minutes and then peel them off. Disinfect the peeled seeds in 70% alcohol for 1 minute, then sterilize them in 0.1% mercuric chloride for 15 minutes. Rinse with bacterial water four times.

[0038] The sterilized seeds were inoculated on 1 / 2MS (Murashige-Skoog) solid medium without sucrose, and germinated at a temperature of 25°C±2°C and a light intensity of 2000Lx.

[0039] (2) Induction of callus

[0040] Cut the hypocotyls of germinated 7-day-old tartary buckwheat aseptic seedlings into 0.5 cm long segments, and inoculate them on the callus-inducing medium. The composition of the callus-inducing medium is: add Agar with a mass concentration of 0.8%, sucrose with a mass concentration of 3%, 1mg / L 2,4-D, 0.5mg / L 6-BA, 10mg / L AgNO 3 ; Cultivate for 15 days at a temperature of 25°C±2°C, 500Lx. Both ends of the hypocotyl expand into a dumbbell shape, and the callus is induced with ...

Embodiment 3

[0046] (1) Obtaining of explants

[0047] Soak the ripe tartar buckwheat seeds in water for 10 minutes and then peel them off. Disinfect the peeled seeds in 70% alcohol for 1 minute, then sterilize them in 0.1% mercuric chloride for 15 minutes. Rinse with bacterial water four times.

[0048] The sterilized seeds were inoculated on 1 / 2MS (Murashige-Skoog) solid medium without sucrose, and germinated at a temperature of 25°C±2°C and a light intensity of 2000Lx.

[0049] (2) Induction of callus

[0050] The hypocotyls of the germinated 7-day-old tartary buckwheat aseptic seedlings were cut into 0.5 cm lengths and inoculated on the callus induction medium (the composition of the callus induction medium was the same as that of Example 1). Cultivate for 15 days at a temperature of 25°C±2°C and 2000 Lx. Both ends of the hypocotyl expand into a dumbbell shape, and callus is induced, with an induction rate of 94.5%. Most of the induced callus are yellow or red callus with loose text...

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Abstract

The invention discloses a method for improving adventitious bud differentiation rate of tartary buck wheat through suspension culture, comprising the following steps: building a solid-liquid-solid culture system of the tartary buck wheat under the in-vitro culture condition, wherein the frequency of inducting the callus tissue is 100%, the adventitious bud differentiation rate is 90.7%, and the rooting rate is 100%; and obtaining the regeneration plants after 8-10 weeks through manually controlling. The method of the invention is simple and easy to implement, reduces the browning degree of the callus tissues of the tartary buck wheat, promotes the differentiation of the adventitious buds, reduces the culture time, and provides the technical base for improving the establishment of the in-vitro high-frequency regeneration system of the tartary buck wheat.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for improving the differentiation of adventitious buds of tartary buckwheat through suspension culture. Background technique [0002] Buckwheat is a dicotyledonous cereal crop of Polygonaceae (Polygonaceae) and the genus Buckwheat (Fagopyrum Mill). The main cultivated species are sweet buckwheat (F. Also known as Tartary buckwheat). Buckwheat is the only food crop containing rutin. It has been gradually discovered and used in research. It is rich in nutrients and can play a good role in weight loss, longevity and intellectual development of children in middle-aged and elderly people. Diseases have obvious therapeutic effects, and contain a variety of vitamins and minerals. The working people in our country have long recognized the medicinal value of Tatar buckwheat. In the great book "Prescriptions for Emergencies" written by Sun Simiao, a famo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 黄萱徐子勤
Owner NORTHWEST UNIV
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