Method for acclimating mother seeds of hypsizygus marmoreus
A technology of female and female species of Jiji mushrooms, applied in botany equipment and methods, horticulture, applications, etc., can solve the problem that the domestication method of the female species of Jiji mushrooms does not appear, and achieve neat fruiting, high content, and good quality Effect
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Embodiment 1
[0014] Select a relatively good mother species test tube for activation, the period is 3 days, connect it to about 15 water agar plates, and cultivate it for 10 days under the condition of 20°C-25°C in a constant temperature incubator. The plates with more developed silk are preserved, and the other plates are eliminated, with an elimination rate of more than 80%. Inoculate the screened strains on PDA plates and culture them in a constant temperature incubator at 20°C-25°C for 10 days, then select plates with no missing corners, well-developed hyphae in the base, and consistent growth for preservation, and eliminate other plates. The elimination rate is over 80%. Connect it to a PDA test tube (barren), and cultivate it for 10 days under the condition of 20°C-25°C in a constant temperature incubator. Select a test tube with no missing corners, well-developed hyphae in the base, and consistent growth, take the front end, and then transfer it to a PDA test tube, and select the o...
Embodiment 2
[0016] Select a relatively good mother seed test tube for activation, the period is 2 days, connect it on about 12 water agar (barren) plates, and cultivate it for 10 days under the condition of 20°C-25°C in a constant temperature incubator. The plates with more developed hyphae in the base are preserved, and the other plates are eliminated, with an elimination rate of more than 80%. Inoculate the screened strains on a nutrient-rich PDA plate, and culture them in a constant temperature incubator at 20°C-25°C for 10 days, then select a plate with no missing corners, well-developed hyphae in the base, and consistent growth for preservation. Other flat panels are eliminated, and the elimination rate is over 80%. Connect it to a PDA test tube and culture it for 10 days in a constant temperature incubator at 20°C-25°C. Take the front end, transfer it to the PDA test tube, and select the one with the best performance as the mother species for preservation.
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