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Phenol degradation fungus and application thereof

A technology of phenol degradation and fungi, applied in the field of environmental microorganisms, to achieve strong environmental tolerance and clean pollution effects

Inactive Publication Date: 2012-04-04
WUHAN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the current research focuses on bacteria, but there are few reports on the biodegradation of phenol by fungi. The fungi that can degrade phenol have been found to be Fusarium floccieferum Fusarium floccieferum (Anselmo et al. Water Sci Technol 1992 , 25:161–168), Aspergillus fumigatus Aspergillus fumigatus (Jones et al. Arch Microbiol 1995, 163:176–181), Graphium sp. ( Santos et al. J Basic Microbiol.2003, 43:238–248), Trichosporon cutaneumhave (Gaal A et al. Arch Microbiol.1981, 130:54–58 and Godjevargova T et al. al. Process Biochem .2003,38:915–20), Penicillium Penicillium sp. (Leitāo. Int J Environ Res Public Health .2009,6:1393–1417. Sumaya Ferreira Guedes et al. Biodegradation.2011, 22:409–419), Paecilomyces variotii (Wang et al. J Hazard Mater. 2010,183:366–371), tropical yeast Candida tropicalis (Jiang Y et al. Biochem Eng J. 2005,24:243–247), filamentous fungi (Santos V L&Linardi V R. Process Biochem.2004,39:1001-1006), white rot fungi white rot fungi (Sanin S. et al. Bull Environ. Contam Toxicol. 2005,75:466–473), these fungi have been shown to have phenol-degrading potential

Method used

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  • Phenol degradation fungus and application thereof
  • Phenol degradation fungus and application thereof
  • Phenol degradation fungus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Screening and identification of phenol-degrading fungal strains

[0028] Inorganic culture medium (mg / L): NaNO 3 2.000 g, K 2 HPO 4 1.000 g, KCl 0.500 g, MgSO 4 .7H 2 O 0.500 g, FeSO 4 .7H 2 O 0.010 g;

[0029] Screening plate medium (mg / L): NaNO 3 2.000 g, K 2 HPO 4 1.000 g, KCl 0.500 g, MgSO 4 .7H 2 O 0.500 g, FeSO 4 .7H 2 O 0.010 g, phenol 0.200 g, agar 18.000 g, H 2 O1000mL;

[0030] Storage medium (mg / L): 200.0 g potatoes, 20.0 g sucrose, 0.100 g phenol, 18.00 g agar, H 2O 1000 mL;

[0031] PDA culture medium (mg / L): potato 200.0 g, sucrose 20.0 g, agar 18.00 g, H 2 O 1000 mL.

[0032] Take 5.0 g of sediment from the East China Sea near Ningbo (longitude 125-128°, latitude 32-35°) in a 250 mL Erlenmeyer flask containing 45 mL of sterile water, shake at 28°C and 150 rpm for 3 h, then statically Set aside for 30 min, transfer 5 mL of the supernatant to 45 mL of inorganic medium containing phenol (100 mg / L), place the Erlenmeyer flask at 28°C,...

Embodiment 2

[0044] Biodegradation of Phenol by Strain PHDE-1

[0045] The strain PHDE-1 was inoculated into 100 mL of sterile screening culture medium containing 100 mg / L phenol, and cultured at 28°C with shaking at 150 rpm for 2 days until the logarithmic phase of growth was used as the seed solution.

[0046] Take 8×1 mL of the above seed solution and inoculate them into 100 mL of sterile phenol containing 200 mg / L, 400 mg / L, 600 mg / L, 800 mg / L, 1000 mg / L, 1200 mg / L, 1400 mg / L, respectively. L, 1600 mg / L simulated wastewater, at 28 ° C, 150 rpm shaking culture, sampling every day, using 4-aminoantipyridine as color reagent, 510 nm colorimetric method to quantitatively determine the concentration of residual phenol, the result show in Figure 7 middle.

[0047] When the phenol content in the simulated wastewater is less than 800 mg / L, phenol can be completely degraded after 10 days; and when the phenol content is 1000 mg / L and 1200 mg / L, the degradation rate of phenol reaches 96% aft...

Embodiment 3

[0049] Biodegradation of Phenol by Strain PHDE-1 at Different pH

[0050] The strain PHDE-1 was inoculated into 100 mL of sterile screening culture medium containing 100 mg / L phenol, and cultured at 28°C with shaking at 150 rpm for 2 days until the logarithmic phase of growth was used as the seed solution.

[0051] Take 6×1 mL of the above seed solution, inoculate them into 100 mL of sterile simulated wastewater containing phenol (800 mg / L) and pH 5.5, 6.0, 6.5, 7.0, 7.5, 8.0 respectively, shake at 28°C and 150 rpm Shake culture for 10 days, using 4-aminoantipyridine as color reagent, 510 nm colorimetric method to quantitatively determine the concentration of residual phenol, the results show that in Figure 8 middle.

[0052] Strain PHDE-1 can biodegrade phenol in a wide pH range, but the degradation rate is affected by pH. At pH 6.5-7.0, the degradation rate of phenol by strain PHDE-1 can reach the maximum.

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Abstract

The invention relates to a phenol degradation fungus. The phenol degradation fungus is preserved in China General Microbiological Culture Collection Center on Aug. 31, 2011, with the preservation number of CGMCCNo.5190. The phenol degradation fungus has the advantage that: phenol degradation fungus strains PHDE-1 provided by the invention have higher environment tolerance, and can grow and reproduce by taking phenol as the only carbon source and energy source under the conditions of wide temperature and pH as well as relatively severe environments so as to remove pollution caused by the phenol.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a strain of phenol-degrading fungus, which can biodegrade phenol under the conditions of pH 5.5-8.0 and temperature 15°C-40°C. Background technique [0002] Phenol is the most common industrial pollutant, which can affect aquatic organisms and cause ecological imbalance. Saha et al. (Saha et al. Bull Environ Contam Toxicol.1999,63: 195-202) reported that the concentration of phenol was as low as 5 to 25 mgL -1 For fish, it is fatal; Kumar et al. (Kumar et al. Biochem Eng J .2005, 22:151–159) reported that phenol is carcinogenic to humans, so wastewater containing phenol must be treated before it can be discharged. Compared with physical and chemical treatment methods, biological treatment of phenol has more advantages because of its low cost and no secondary pollution. The biodegradation of phenol is the result of microbial activities such as bacteria, fun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A62D3/02C02F3/34C12R1/645A62D101/28C02F101/34
Inventor 户业丽程波
Owner WUHAN INSTITUTE OF TECHNOLOGY
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