Method for conveying nucleic acid to vivo tissue cells by flexible electrode chip

Abstract

The invention discloses a method for conveying nucleic acid to vivo tissue cells by a flexible electrode chip, comprising steps of: introducing nucleic acid into vivo tissues to distribute nucleic acid around the vivo tissue cells; attaching the flexible electrode chip on the surface of the vivo tissues and applying electrical field to the vivo tissues to let nucleic acid enter into the inside of the vivo tissue cells. The flexible electrode chip comprises a flexible substrate which can bend and can be made into different shaped according to the appearance of the vivo tissues and micro-electrodes which are made on the substrate and placed in pairs.

Description

technical field [0001] The present invention relates to a method for transporting nucleic acid to the inside of cells in living tissue, more specifically, the present invention relates to a method for using a large-area uniform electric field produced by a metal electrode fabricated on a flexible film material to affect the nucleic acid in living tissue. A method in which cells are electroporated for the delivery of nucleic acids. Background technique [0002] With the rapid development of gene technology, nucleic acid drugs have become one of the most promising methods for the treatment of tumors and other diseases. However, the biggest obstacle to the practical application of nucleic acid drugs is the lack of effective methods for transporting nucleic acids into cells. Unlike traditional chemical drugs, nucleic acids cannot penetrate cell membranes, so they cannot be administered orally or injected like chemical drugs. Most of the currently disclosed methods are to enable...

AI Technical Summary

Problems solved by technology

This method has two major defects: firstly, the penetration of the tissue by the needle-shaped electrodes will cause greater physical damage to the living tissue, and the more the needle-shaped electrodes are, the more serious the damage will be; The electric field is not uniform, which will cause cells in some areas to fail to be electroporated due to insufficient electric field strength, while at the same time, cells in some areas will die due to excessive electric field strength

This method solves the problem of physical damage to living tissue in the above-mentioned first method, but this method also has two major defects: first, because the barrier of the skin needs to be penetrated, the required electric field on the skin surface needs to be It is greater than the electric field inside the tissue, and the scale of the current planar electrodes is relatively large (several millimeters), which means that the voltage of hundreds of volts or even thousands of volts is fatal to living tissues; secondly, the current planar electrodes are large Some are made on rigid substrates (such as silicon wafers, glass wafers), etc., which cannot adapt to changes in the shape of living tissues at all, so they cannot be practically applied to nucleic acid transport.

[0011] In addition to the defects stated separately, the above two methods also have a common defect: because of the lack of biocompatible materials, high voltage and harmful physical penetration methods, none of the existing methods can be used. For implantable devices, it can only be applied to shallow tissues very close to the body surface, which greatly limits the scope of application of existing methods

Images