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Sorbent for endotoxins

An sorbent and endotoxin technology, which is applied in solid sorbent liquid separation, suction equipment, selective adsorption, etc., can solve the problems of complex production methods, high patient mortality, high production costs, etc. The effect of saving, improving the chance of survival

Inactive Publication Date: 2012-02-22
FRESENIUS MEDICAL CARE DEUTSCHLAND GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Other disadvantages arise from expensive and complex production methods and the higher production costs associated therewith
[0016] Although the mortality rate of patients suffering from endotoxin poisoning (especially sepsis) has been reduced by the clinical application of the above-mentioned Toraymyxin adsorption module, the death rate of patients suffering from severe sepsis and septic shock despite maximal treatment rate is still very high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0041] 1. Example 1: Production of sorbent (adsorbent) according to the present invention

[0042] In order to produce the sorbent according to the present invention, polymyxin B is used to coat neutral and hydrophobic polystyrene-divinylbenzene copolymers with different pore sizes, that is, polymyxin B via polystyrene -The hydrophobic interaction on the outer surface and the inner surface of the divinylbenzene copolymer is adsorbed.

[0043] 1.1. Provide neutral and hydrophobic polymers:

[0044] Table 1 lists the various average pore sizes of the polystyrene-divinylbenzene copolymer (referred to as "polymer" or "support" or "uncoated adsorbent" for short).

[0045] Table 1: Polystyrene-divinylbenzene copolymer

[0046] Name

Average pore size [nm]

#1822

15-20

#1823

15-20

#1824

30-40

#1825

80-100

#1826

80-100

[0047] The particle size of the polymer is 5μm + / -3-4μm.

[0048] 1.2. Coating polymyxin on polystyrene-divinylbenzene copolymer:

[0049] The polymers ...

example 2

[0055] 2. Example 2: Adsorption of endotoxin-batch test

[0056] The polymer average pore size (PS) of #1825+PMB and #1826+PMB coated polymyxin B adsorbents is 80-100nm (see Example 1-Table 2), which is consistent with the corresponding #1825 and The #1826 uncoated adsorbent was compared for endotoxin binding.

[0057] 2.1. Preparation of adsorbent and adsorbent:

[0058] Prepare the following adsorbents (PS=80-100) according to the scheme of Example 1:

[0059] 1)#1825

[0060] 2)#1826

[0061] 3) #1825+PMB (PMB coated)

[0062] 4) #1826+PMB (PMB coated)

[0063] As described in 1.2, rinse the polymyxin B-coated and uncoated adsorbents 5 times with NaCl without a pyrogen. The 50% adsorbent suspension is finally produced in NaCl without a heat source.

[0064] 2.2. Heparin plasma:

[0065] Obtain 25ml heparin plasma from the donor (5x 9ml whole blood draw).

[0066] 2.3. Endotoxin solution:

[0067] LPS Pseudomonas aeruginosa (Sigma, L7018, lot number 109H4043). The stored in the -70℃ mi...

example 3

[0083] 3. Example 3: Adsorption of endotoxin-batch test

[0084] The average pore size (PS) is 15-20nm or 30-40nm coated polymyxin B adsorbent #1822+PMB, #1823+PMB and #1824+PMB and the corresponding uncoated adsorbent# 1822, #1823, and #1824 are compared for endotoxin binding.

[0085] 3.1. The adsorption body and the preparation of the adsorption body:

[0086] Prepare the following adsorbents according to the scheme of Example 1:

[0087] 1)#1822

[0088] 2)#1823

[0089] 2)#1824

[0090] 3) #1822+PMB (PMB coated)

[0091] 4) #1823+PMB (PMB coated)

[0092] 5) #1824+PMB (PMB coated)

[0093] As described in 1.2, rinse the polymyxin B-coated and uncoated adsorbents 5 times with NaCl without a pyrogen. The 50% adsorbent suspension is finally produced in NaCl without a heat source.

[0094] 3.2. Heparin plasma and endotoxin solution: Corresponds to parts 2.2 and 2.3.

[0095] 3.3. Test batch:

[0096] In the batch tested, the endotoxin solution was further diluted to a final concentration ...

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Abstract

The invention relates to a sorbent for removing endotoxins from a biological liquid, the sorbent comprising a water-insoluble, porous support and polymyxin which is immobilized on the support, said support having a neutral, hydrophobic surface and the polymyxin being immobilized on the surface of the support via hydrophobic interaction. The sorbent is especially used in extracorporeal blood purification, especially for the treatment of individuals suffering from a sepsis.

Description

Technical field [0001] The present invention relates to a sorbent for removing endotoxins from biological fluids. The sorbent has a porous carrier that is insoluble in water and polymyxin fixed on the carrier. Background technique [0002] Endotoxin is lipopolysaccharide (LPS) in the cell wall of Gram-negative bacteria and is released by cell lysis. In fact, lipopolysaccharide is the most common lipid component in the outer cell membrane of Gram-negative bacteria. Endotoxin is a pyrogenic substance, that is, if the endotoxin reaches the human body in the process of poisoning caused by microorganisms, for example, the infected individual will have a severe inflammatory reaction and fever, and show a systemic effect. The presence of endotoxin in the blood circulation can lead to uncontrollable activation of immune cells and cause imbalance in the coagulation system. Depending on its concentration, it can cause sepsis. The symptoms of sepsis include (but are not limited to) high f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/32B01J20/26B01J20/28A61M1/36B01D15/00B01J20/285
CPCA61M1/3679B01D15/00B01D15/08B01J20/28004B01J20/28023B01J20/28085B01J20/321B01J20/3253B01J20/3255B01J2220/58
Inventor 迪特尔·法尔肯哈根维多利亚·韦伯
Owner FRESENIUS MEDICAL CARE DEUTSCHLAND GMBH
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