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Composite bacillus cottonseed meal fermenting agent and preparation method thereof

A compound Bacillus and cotton meal fermentation technology, which is applied in the field of microbial fermentation, can solve the problems of low number of viable bacteria in the fermentation agent, difficulty in stabilizing the fermentation effect, and short storage time, so as to improve animal immunity and eliminate anti-nutrition factor, the effect of simple production process

Inactive Publication Date: 2012-02-15
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since most of the above strains cannot produce spores during the fermentation process, there are disadvantages such as the number of viable bacteria in the fermented bacterial agent produced is not high, the storage time is not long, and the fermentation effect is difficult to stabilize, or the fermentation strain is single, the production process is complicated, and the production cost is high.
For the technology of detoxification treatment using Bacillus natto, Bacillus coagulans, Bacillus lateralis and Bacillus subtilis to ferment cotton meal with composite fermentation agent, there is no literature report at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Prepare various liquid strains:

[0024] Liquid culture medium (4 parts): add glucose 30g, bean cake powder 15g, (NH 4 ) 2 SO 4 4g, KH 2 PO 4 4g, MgSO 4 ·7H 2 O 1.5g, NaCl 5g, and CaCO 3 2g, initial pH7.2;

[0025] Take 1 strain of Bacillus subtilis and inoculate a ring of slanted strain cells into a liquid strain culture medium, and culture it on a shaking table at 37°C and 200r / min for 60h, so that the spore formation rate of the cells in the seed liquid can reach 94.7% , 80°C constant temperature water bath for 12 minutes;

[0026] Take 1 strain of Bacillus lateralis and inoculate a ring of slanted strain cells into the liquid strain culture medium, and culture it on a shaking table at 37°C and 200r / min for 60 hours, so that the spore formation rate of the cells in the seed liquid can reach 92.6% , 80°C constant temperature water bath for 12 minutes;

[0027] Take 1 strain of Bacillus natto and inoculate a ring of slanted strain cells into the liquid ...

Embodiment 2

[0040] 1. Prepare various liquid strains:

[0041] Liquid culture medium (4 parts): 35g of glucose, 12g of bean cake powder, (NH 4 ) 2 SO 4 6g, KH 2 PO 4 3g, MgSO 4 ·7H 2 O 2.5g, NaCl3g, and CaCO 3 4g, initial pH7.5;

[0042] Take 1 strain of Bacillus subtilis and inoculate a ring of slanted strain cells into a liquid strain culture medium, and culture it on a shaking table at 40°C and 180r / min for 48 hours, so that the spore formation rate of the cells in the seed liquid can reach 92.7% , 80°C constant temperature water bath for 10 minutes;

[0043] Take 1 strain of Bacillus lateralis and inoculate a ring of slant strain cells into the liquid strain culture medium, and culture it on a shaking table at 40°C and 180r / min for 48 hours, so that the spore formation rate of the cells in the seed liquid can reach 91.3%. , 80°C constant temperature water bath for 10 minutes;

[0044] Take 1 strain of Bacillus natto and inoculate a ring of slanted strain cells into the li...

Embodiment 3

[0057] 1. Prepare various liquid strains:

[0058] Liquid culture medium (4 parts): add glucose 40g, bean cake powder 10g, (NH 4 ) 2 SO 4 2g, KH 2 PO 4 2g, MgSO 4 ·7H 2 O 1g, NaCl4g, and CaCO 3 3g, initial pH7.4;

[0059] Take 1 strain of Bacillus subtilis, Bacillus lateralis, Bacillus natto, and Bacillus coagulans to inoculate a ring of slant strain cells respectively in the liquid culture medium, shake at 39°C and 160r / min Bed shaking culture for 52 hours, so that the sporulation rate of the cells in the seed liquid reached 95%, and treated in a constant temperature water bath at 80°C for 15 minutes, and each liquid strain was prepared respectively;

[0060] (2) Fermentation:

[0061] The above-mentioned Bacillus coagulans liquid strains were inoculated in the Bacillus coagulans fermentation medium, the culture temperature was 37°C, the inoculum size was 4%, the stirring speed was 170r / min, and the fermentation culture time was 50h.

[0062] Bacillus coagulans fer...

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Abstract

The invention discloses a composite bacillus cottonseed meal fermenting agent and a preparation method thereof. The preparation method comprises the following steps of: performing expanding culture on strains of bacillus subtilis, bacillus natto, brevibacillus laterosporus and bacillus coagulans by a liquid fermentation method; inoculating into a fermentation tank to ferment; adding a carrier to adsorb; drying at low temperature; and mixing in a ratio to prepare the composite bacillus cottonseed meal fermenting agent, the total microbial content of the fermenting agent is more than or equal to 100*108 CFU / G. The composite bacillus cottonseed meal fermenting agent can effectively inhibit growth of miscellaneous bacteria in the fermentation process of the cottonseed meal, reduce the free gossypol content of the cottonseed meal and increase the protein content and the free amino acid content of the cottonseed meal, and has the characteristics of simple preparation process, stable and reliable production quality, low cost and the like.

Description

Technical field: [0001] The invention belongs to the technical field of microbial fermentation, and relates to a preparation method of a cotton meal starter, in particular to a preparation method of a compound bacillus cotton meal starter. Background technique: [0002] Cotton meal is a by-product of cottonseed oil extraction, accounting for about 30% of the total annual output of various cakes in my country. Cotton meal is an excellent protein feed resource, and its output is large and nutritious. At present, the protein content of cottonseed meal used as feed raw material is generally 44.32%, which is second only to the protein content of soybean meal at 49.48%, and higher than that of rapeseed meal at 36.04%. Cotton meal can be hydrolyzed to obtain 17 kinds of amino acids, among which the content of lysine is low, 1.4-2.13%, while the content of arginine is high, 3.94-4.98%. The ratio of lysine to arginine is far more than The ideal value of 100:120 has been achieved. ...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/20A23K1/16A23K1/14A23L1/015C12R1/125C12R1/07A23K10/12A23K10/30A23L5/20
Inventor 兰时乐王红权肖调义赵玉蓉金红春毛小伟
Owner HUNAN AGRICULTURAL UNIV
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