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Polydimethylsiloxane (PDMS)-based three-dimensional single cell culture chip and controllable preparation method thereof

A single-cell, three-dimensional technology, applied in tissue cell/virus culture devices, biochemical equipment and methods, specific-purpose bioreactors/fermenters, etc., can solve problems such as poor pattern shape and pattern property stability , to achieve long-term stability of graph properties and graph structures

Inactive Publication Date: 2013-06-05
NORTHWESTERN POLYTECHNICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a stable three-dimensional single-cell culture chip in order to solve the problem of poor pattern shape and pattern property stability existing in the existing single-cell micro-patterned culture chip

Method used

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  • Polydimethylsiloxane (PDMS)-based three-dimensional single cell culture chip and controllable preparation method thereof
  • Polydimethylsiloxane (PDMS)-based three-dimensional single cell culture chip and controllable preparation method thereof
  • Polydimethylsiloxane (PDMS)-based three-dimensional single cell culture chip and controllable preparation method thereof

Examples

Experimental program
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Embodiment 1

[0024] The chip in this example is used to culture osteoblast MC3T3-E1 to conduct research on cell contact connection. MC3T3-E1 average diameter 20μm, average height h 0 is 3 μm, the average synapse length l 0 is 20 μm.

[0025] refer to Figure 1 ~ Figure 3 . The three-dimensional single-cell culture chip used for culturing MC3T3-E1 in this embodiment includes circular cavities 1 distributed in an array, and microgrooves 3 are connected between adjacent cavities 1, and the depth of microgrooves 3 is the same as that of the cavities. The height h of body 1 is 4 μm; the bottom of cavity 1 contains nanoprotrusions; the diameter of cavity 1 The distance l between two adjacent cavities 1 is 50 μm; the micro-grooves 3 are evenly distributed at 90°, and the width of the micro-grooves 3 is a=2 μm.

[0026] In this embodiment, the preparation method of the PDMS-based three-dimensional single-cell culture chip is completed by using MEMS technology and replication molding techno...

Embodiment 2

[0041] The chip in this example is used to culture human mammary epithelial cells HMEC to conduct research on cell non-contact connection. HMEC average diameter 45μm, average height h 0 is 5 μm, the average synapse length l 0 is 10 μm.

[0042] refer to Figure 1 ~ Figure 3 . The three-dimensional single-cell culture chip used for HMEC culture in this embodiment includes circular cavities 1 distributed in an array, and there are microgrooves 3 connected between adjacent cavities 1, and the depth of the microgrooves 3 is the same as that of the cavities 1. The height h is h=7μm; the bottom of cavity 1 contains nano-protrusions; the diameter of cavity 1 The distance between two adjacent cavities 1 is l=80 μm; the micro-grooves 3 are evenly distributed at 90°, and the width of the micro-grooves 3 is a=5 μm.

[0043] In this embodiment, the preparation method of the PDMS-based three-dimensional single-cell culture chip is completed by using MEMS technology and replication ...

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Abstract

The invention discloses a three-dimensional single cell culture chip realized based on a micro-fabrication technology, and belongs to bio micro electro mechanical systems (Bio-MEMS). The chip comprises cavities distributed in an array; adjacent cavities are connected with each other through a micro groove; the depth of the micro groove is consistent with the height of the cavities; and the bottoms of the cavities are provided with nano projections. According to difference of adhesion of cells on the surface of blank polydimethylsiloxane (PDMS) and on the surface of PDMS with a nano micro morphology structure and by combining a surface topology pattern, the chip realizes cell patterning culture. The chip can be used for cell culture without chemical or biological reagents, overcomes a defect of low stability of the conventional patterning method, has the characteristics of durable and stable pattern, controllable preparation process and the like, and can meet the requirement of batch production. Meanwhile, a patterning design that the cavities are connected through micro grooves is adopted, and the research on the interaction of cells can be realized.

Description

[0001] Field [0002] The invention relates to a three-dimensional single-cell culture chip realized based on microfabrication technology, and belongs to the field of biological micro-electromechanical systems (Bio-MEMS). Background technique [0003] As an experimental tool for anchorage-dependent cell research and behavior control, micropatterned single-cell culture chips have broad applications in basic cell biology, life sciences, tissue engineering, drug screening, and cell-based biosensors. prospect. [0004] The typical representative used to achieve cell micropatterning is microcontact printing technology, which is a method of indirectly realizing cell patterning with the help of intermediate patterns. The stability of intermediate patterns is directly related to the success or failure of cell pattern construction. Due to the problems of protein "inactivation" and protein desorption, and the lack of high density of self-assembled molecular layers, the stability of cel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/34B29C39/02B29C39/22B29B7/00B29B13/06
CPCC12M23/12
Inventor 叶芳常洪龙何洋高洁续惠云谢丽张卫菊黎永前
Owner NORTHWESTERN POLYTECHNICAL UNIV
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