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Enzyme electrode for detecting hydrogen peroxide and preparation method of enzyme electrode

A hydrogen peroxide, enzyme electrode technology, applied in the field of electrochemical analysis and detection, can solve the problems of stability, sensitivity can not be very satisfactory and so on

Inactive Publication Date: 2012-01-18
CAPITAL NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Experiments show that after the electrode is stored at 4°C for 20 days, its voltammetric response can maintain about 80%, but its stability, sensitivity, etc. still cannot achieve very satisfactory results

Method used

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  • Enzyme electrode for detecting hydrogen peroxide and preparation method of enzyme electrode
  • Enzyme electrode for detecting hydrogen peroxide and preparation method of enzyme electrode

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Preparation of MWNT-CoPc / Cat / Nafion Modified Glassy Carbon Electrode

[0020] (1) Mix 1g of phthalonitrile, 0.25g of anhydrous cobalt chloride, 0.1g of multi-walled carbon nanotubes (MWNT), 1mL of DBU and 40mL of n-pentanol by ultrasonic mixing, heat and stir at 180°C for 2h, filter the product and use Washing with anhydrous methanol and drying at 80°C for 4 hours to obtain dark black powder MWNT-CoPc;

[0021] (2) Weigh 3 mg MWNT-CoPc and ultrasonically disperse it in 1 mL of absolute ethanol; drop-coat the uniformly dispersed composite modifier on the surface of a clean glassy carbon electrode (GCE), and then drop-coat 2 μL of hydrogen peroxide in turn after drying Enzyme (Cat) and 1 μL of 5% Nafion solution were dried at room temperature to obtain a MWNT-CoPc / Cat / Nafion electrode.

Embodiment 2

[0023] Preparation of MWNT-CoPc / Cat / Nafion Modified Glassy Carbon Electrode

[0024] (1) 0.6g of phthalonitrile, 0.18g of anhydrous cobalt chloride, 0.1g of multi-walled carbon nanotubes (MWNT), 0.6mL of DBM and 30mL of nitrobenzene were ultrasonically mixed, heated and stirred at 160°C for 3h, the product After filtration, wash with anhydrous methanol, and dry at 100°C for 2 hours to obtain dark black powder MWNT-CoPc;

[0025] (2) Weigh 3 mg MWNT-CoPc and ultrasonically disperse it in 1 mL of absolute ethanol; drop-coat the uniformly dispersed composite modifier on a clean glassy carbon electrode (GCE) surface, and then drop-coat 4 μL of hydrogen peroxide in turn after drying Enzyme (Cat) and 2 μL of 5% Nafion solution were dried at room temperature to obtain a MWNT-CoPc / Cat / Nafion electrode.

Embodiment 3

[0027] Preparation of MWNT-CoPc / Cat / Nafion Modified Glassy Carbon Electrode

[0028] (1) Ultrasonically mix 1.5g phthalonitrile, 0.35g anhydrous cobalt chloride, 0.18g multi-walled carbon nanotubes (MWNT), 2mL DBM and 60mL trichlorobenzene, heat and stir at 160°C for 2h, the product After filtration, wash with anhydrous methanol, and dry at 100°C for 2 hours to obtain dark black powder MWNT-CoPc;

[0029] (2) Weigh 6 mg of MWNT-CoPc and ultrasonically disperse it in 2 mL of absolute ethanol. The uniformly dispersed composite modifier was drop-coated on the surface of a clean glassy carbon electrode (GCE), and after drying, 4 μL of catalase (Cat) and 2 μL of 5% Nafion solution were sequentially drip-coated and dried at room temperature to obtain MWNT-CoPc / Cat / Nafion electrodes.

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Abstract

The invention relates to a preparation method of a carbon nano tube and metal phthalocyanine / enzyme modified glassy carbon electrode for detecting hydrogen peroxide, belonging to the technical field of electrochemical analysis and detection. A multi-wall carbon nano tube and cobalt phthalocyanine composite material is prepared by using an in-situ synthesis method, a carbon nano tube and metal phthalocyanine / hydrogen peroxide enzyme modified glassy carbon electrode is prepared by using a dropwise-coating method, a novel electrochemical biosensor is manufactured, and H2O2 can be detected rapidly, stably, flexibly and accurately, and interference of common substances such as ascorbic acid and the like on the measurement of H2O2 can be eliminated.

Description

technical field [0001] The invention relates to an enzyme electrode and a preparation method thereof, in particular to a carbon nanotube-metal phthalocyanine / enzyme-modified glassy carbon electrode for detecting hydrogen peroxide and a preparation method thereof, belonging to the technical field of electrochemical analysis and detection. Background technique [0002] Hydrogen peroxide (H 2 o 2 ) is the product of an enzymatic reaction involving peroxidase, H 2 o 2 The determination of H 2 o 2 The detection has attracted more and more attention. Currently, detection of H 2 o 2 Titration, chemiluminescence, fluorescence, and electrochemical methods are generally used. Among them, electrochemical analysis methods are widely studied because of their high sensitivity, good linear range, and fast and stable response signals. Enzyme electrodes are often not widely used due to their complex preparation process, poor stability, long-term storage, and greatly reduced sensitivi...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/416
Inventor 左霞张会李楠
Owner CAPITAL NORMAL UNIVERSITY
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