A primer-solid phase carrier complex and its preparation and method for sequencing

Abstract

The invention relates to the biotechnical field, and provides a primer-solid-phase carrier composite and a preparation method and application thereof. The primer-solid-phase carrier composite comprises primers and a solid-phase carrier, wherein the solid-phase carrier comprises a first solid-phase carrier and second solid-phase carriers; the first solid-phase carrier is combined with at least one second solid-phase carrier; and the second solid-phase carrier is combined with at least one primer. The primer-solid-phase carrier composite has the characteristic of high primer combination density, and can greatly improve the polymerase chain reaction (PCR) amplification efficiency on the solid-phase surface and reduce the operation requirements on operating personnel in the subsequent amplification reaction.

Description

technical field [0001] The present invention relates to the field of biotechnology, more specifically, relates to a primer-solid phase carrier complex and its preparation and sequencing method. Background technique [0002] High-throughput sequencing technology is a revolutionary change to traditional sequencing. It can sequence hundreds of thousands to millions of DNA molecules at a time. At the same time, high-throughput sequencing enables detailed and comprehensive analysis of the transcriptome and genome of a species. become possible. At present, there are two main ways for high-throughput sequencing platforms to process samples, namely, sample processing by slide bridge PCR and sample processing by emulsion PCR. [0003] The principle of slide bridge PCR for sample processing is as follows: primers complementary to asymmetric adapters are randomly distributed on the surface of the chip, and asymmetric adapters are added to both ends of the single-stranded DNA, and the ...

AI Technical Summary

Problems solved by technology

However, the technology of "water injection into oil" requires professionals to operate, and has high technical requirements for operators, and it is very easy to fail. At the same time, a variety of special instruments (such as emulsion shakers, PCR instrument, emulsion breaker, etc.), in addition, the utilization efficiency of emulsion PCR on magnetic beads is low, and after the amplification is completed, special steps are required to enrich the magnetic beads with amplified products, and then the subsequent sequencing reaction can be carried out

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