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High-sensitivity polyion-selective electrode and testing method thereof

A test method and selective technology, applied in the field of sensors, can solve the problems that the electrode can no longer obtain a response signal, the sensitivity of the pulse electrode is not enough, etc., to achieve the effect of potentiometric titration measurement, avoid batch differences, and reduce test costs.

Active Publication Date: 2012-01-11
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, once the electrode is used for the determination of main ions, the remaining main ions in the membrane will make the electrode unable to obtain a similar response signal to the first measurement.
Although the polyion pulse electrode based on current control realizes the repeated use of polyion sensors, the sensitivity of the pulse electrode is not as good as that of the traditional electrode, so it is difficult to replace the traditional zero-current polyion selective electrode.

Method used

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  • High-sensitivity polyion-selective electrode and testing method thereof
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  • High-sensitivity polyion-selective electrode and testing method thereof

Examples

Experimental program
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Embodiment 1

[0033] Highly sensitive regenerative protamine selective electrode.

[0034] The potential sensor includes a working electrode 1, an external reference electrode 2, an internal reference electrode 3 and a potentiometer 4. The potentiometer 4 is respectively connected to the internal reference electrode 3 and the external reference electrode 4 through wires, and the internal reference electrode 3 is inserted into the In the working electrode 1 filled with liquid 8, the working electrode 1 and the external reference electrode 2 are inserted into the detection cell 5 filled with the detection liquid 6. The working electrode 1 is an electrode carrier with a polymer sensitive film 7 fixed on it. A magnetic stirrer 9 with a stirring effect is provided, and a magnetic stirrer is provided at the bottom of the detection pool to rotate the magnetic stirrer. The electrode carrier on which the polymer sensitive membrane 7 is fixed is a polyvinyl chloride tube.

[0035]Preparation of work...

Embodiment 2

[0042] The difference from Example 1 is: highly sensitive and reproducible sodium polysulfate selective electrode.

[0043] Preparation of working electrode: the anion exchanger tri-dodecylmethylammonium chloride doped polymer film is used as the working electrode sensitive film, the silver-silver chloride electrode is used as the internal and external reference electrode, and the internal reference electrode and the electrochemical workstation The working electrode of CHI760D is connected to the joint, the external reference electrode is connected to the reference electrode joint, and the "open circuit potential-time" technique is selected to measure the potential value.

[0044] The preparation of the working electrode sensitive film is: according to the mass percentage 1.5%: 66%: 32.5%, a total of 345 mg of tri-dodecylmethyl ammonium chloride, polyvinyl chloride, and dioctyl sebacate are dissolved in 3.5 mL of tetrahydrofuran In the solution, place it in a flat-bottomed gla...

Embodiment 3

[0047] The difference from Example 1 is:

[0048] Determination of Heparin Using a Highly Sensitive Renewable Protamine Selective Electrode.

[0049] After washing the protamine on the surface of the electrode preactivated by protamine with deionized water, transfer the electrode into 4mL of 0.12M NaCl containing heparin, and act for 30min to activate it, which is the protamine selective electrode for titration determination of heparin .

[0050] To measure the unknown concentration of heparin, use the above-mentioned selective electrode, after turning on the magnetic stirring, add 1 μL of 0.2 mg / mL protamine solution to the above 4 mL of 0.12M NaCl containing 0.025-10 μg / mL heparin every 30 s. The potential change was recorded, and the addition of protamine was stopped when the potential change did not exceed 0.2mV within 30s. The standard working curve of heparin can be obtained by plotting the protamine concentration at the titration end point of the obtained potential ti...

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Abstract

The invention relates to a sensor, in particular to a high-sensitivity renewable potential sensor for multi-charge ions (polyion-selective electrode). Primary ions (target polyions) of an ion-exchanger-doped polymer film are pre-activated, the electrode is transferred into background electrolyte for activation after pre-activation, and thus the high-sensitivity polyion-selective electrode is obtained. The electrode can be regenerated by the high-concentration primary ions in testing solution during testing or after testing. For high sensitivity, the primary ions to be tested are inhibited in the external surface layer of the film based on the primary ion flow from a sensitive film phase to a film interface. For renewability, the primary ions need not to be completely eliminated from the film. No complex rotary disc electrode device or external current is needed for the sensor, the sensitivity and the renewability of the electrode are significantly improved, and the electrode also has great significance in the direct potential testing of polyions, the titration testing with a polyion electrode as a signal converter, the enzyme testing and the immunoassay.

Description

technical field [0001] The invention relates to a sensor, in particular to a highly sensitive polyion selective electrode and a testing method thereof. Background technique [0002] Polymer liquid membrane polyion selective electrode is a kind of multi-charge ion potential sensor with low cost and simple operation. This type of sensor includes a polycation selective electrode and a polyanion selective electrode, wherein the active site of the sensitive membrane of the polycation selective electrode is a cation exchanger (borate derivative or dinonyl naphthalene sulfonic acid or its salt), The active sites of the polyanion selective electrode sensitive membrane are anion exchangers (quaternary ammonium salt organic compounds). The working principle of the polyion selective electrode is: when there are polyions in the test solution, the polyions will form ion pairs with the ion exchanger in the sensitive membrane, and then be extracted into the sensitive membrane. At the sam...

Claims

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Application Information

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IPC IPC(8): G01N27/31G01N27/26
Inventor 秦伟王学伟
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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