Symbiosis composition and its preparation method
A composition and mixture technology, applied in food preparation, food science, animal feed, etc., can solve the problem of unsatisfactory symbiotic composition and achieve good homogeneity
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Embodiment 1
[0151] Example 1 - Preparation of Composition
[0152] The compositions of the present invention are prepared from the following compounds:
[0153] - cane molasses in crude condition from the Gardel Sugar Factory (Guadeloupe) especially for the type of food prepared in question;
[0154] - Dehydrated and encapsulated live microorganisms: Bifidobacterium longum type bacteria, sold by Lallemand (France) under the name Probiocap Rosell-175ME; the microbiological characteristics of this compound provided by Lallemand are as follows: Bifidobacterium longum > 5 × 10 10 cfu / g; Staphylococci 3 cfu / g; saprophytes 4 cfu / g; Pseudomonas 4 cfu / g; Salmonellae <1 cfu / 25g; Escherichia coli <1 cfu / g.
[0155] - Polysaccharide fiber: Nutriose (Roquette, France).
[0156] Microbiological characterization of molasses was performed prior to its use. For this, dilute molasses to 1 / 10 in LT100 broth and inoculate:
[0157] -To count the total aerobic bacteria, inoculate on Trypticase Soybea...
Embodiment 2
[0167] Example 2 - Effect of Compositions on Cell Viability
[0168] The combination obtained in Example 1 was evaluated from the viewpoint of viability of human colorectal cancer cells (Caco-2 line, clone TC-7) cultured in monolayer by the reduced MTT method (brominated thiazolyl blue tetrazolium) things. In DMEM supplemented with penicillin (Sigma, 50IU / mL), streptomycin (Sigma, 50mg / mL), gentamicin (Sigma, 10mg / mL) and fetal bovine serum (Sigma, 10%, v / v) Cell monolayers were grown to confluence in Acane MEM medium.
[0169] Dilute the final composition to 1 μg / mL, 10 μg / mL, 100 μg / mL, 1 mg / mL, 10 mg / mL, and 100 mg / mL, and place the cells at 37°C, containing 5% CO 2 Stored in a humid atmosphere for 48 days. EGF (epidermal growth factor) was used as a reference for the TC-7 cell proliferation assay.
[0170] Cell viability was measured according to the MTT reduction method. The reduction of MTT to Formazan Blue was assessed by measuring the absorbance, which is direct...
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