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Recombinant plasmid for degrading nitrobenzene, gene engineering bacteria and preparation method thereof

A technology of genetically engineered bacteria and recombinant plasmids, applied in genetic engineering, methods based on microorganisms, botanical equipment and methods, etc., can solve problems such as high cost and secondary pollution, and achieve the effect of improving the biodegradability of water bodies

Inactive Publication Date: 2011-12-21
ZHEJIANG UNIV
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Problems solved by technology

The use of traditional physical and chemical processes is not only costly, but also easily causes secondary pollution, leaving chemical agents in the treated water and generating new pollutants

Method used

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  • Recombinant plasmid for degrading nitrobenzene, gene engineering bacteria and preparation method thereof
  • Recombinant plasmid for degrading nitrobenzene, gene engineering bacteria and preparation method thereof
  • Recombinant plasmid for degrading nitrobenzene, gene engineering bacteria and preparation method thereof

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Embodiment Construction

[0017] One aspect of the present invention provides a recombinant plasmid carrying the coding sequences of nitrophenyl nitroreductase, hydroxyaniline mutase and 2-aminophenol-1,6-dioxygenase, nitrophenyl nitrate The coding sequences of base reductase, hydroxyaniline mutase and 2-aminophenol-1,6-dioxygenase are shown in SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3 respectively. .

[0018] Another aspect of the present invention also provides a genetically engineered bacterium that efficiently degrades nitrobenzene, the genetically engineered bacterium contains the above-mentioned recombinant plasmid (such as figure 1 shown).

[0019] The method for constructing the above-mentioned recombinant plasmid comprises the following steps:

[0020] 1. Obtain the coding genes encoding the enzymes (nitrobenzene nitroreductase, hydroxyaniline mutase and 2-aminophenol-1,6-dioxygenase) required for the degradation of nitrobenzene by PCR;

[0021] 2. Link the above genes into the vector pET-21...

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Abstract

The invention discloses a recombinant plasmid for degrading nitrobenzene, gene engineering bacteria and a preparation method thereof. The recombinant plasmid has gene sequences which are represented by SEQ ID No.1, SEQ ID No.2 and SEQ ID No.3 between a locus BglII and a locus XhoI. The nitrobenzene degrading engineering bacteria strain PET-NB disclosed by the invention can effectively degrade nitrobenzene in solution to form a ring-opening product; and thus, the biochemical quality of water is improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a recombinant plasmid for degrading nitrobenzene, a genetically engineered bacterium for efficiently degrading nitrobenzene, and a method for preparing the recombinant plasmid and the genetically engineered bacterium. Background technique [0002] At present, the problem of water pollution is becoming more and more serious. The pollutants in natural water bodies, especially the pollution of aromatic refractory compounds such as nitrobenzene, have seriously threatened the safety of human beings and other organisms. The use of traditional physical and chemical processes is not only costly, but also easily causes secondary pollution, leaving chemical agents in the treated water and generating new pollutants. Contents of the invention [0003] The object of the present invention is to provide a recombinant plasmid for degrading nitrobenzene, genetically engineered bacteria ...

Claims

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Application Information

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IPC IPC(8): C12N15/63C12N15/52C12N15/53C12N1/21C02F3/34C12R1/19C02F101/38
Inventor 金丹凤赵宇华贾小明刘双江刘丹凤
Owner ZHEJIANG UNIV
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