Key marker sites and applications of muc13 gene affecting susceptibility/resistance to f4ac diarrhea in piglets

Abstract

The invention discloses a susceptible / resistant MUC13 (mucoprotein) gene key sign locus affecting piglet F4ac diarrhea and an application thereof in pig genetic improvement. A large scale of white Duroc*Erhualian Boars resource groups and 15 Chinese and foreign pig species distant groups are subjected to complete-genome scanning linkage position, target zone high-density SNP (Single Nucleotide Polymorphism) multi-point linkage precise positioning, breakpoint recombination analysis and distant group associativity analysis based on linkage unbalance to finally lock a coding ETEC (Enterotoxigenic escherichia coil) F4ac receptor target gene-MUC13 gene and separate and colon the MUC13 gene. 15 key mutantsites are indentified in the MUC13 gene. The 15 mutantsites and ETEC F4ac in vitro adhesive phenotype are basically mutually separated in 144 pure breed Duroc, Landrace and big white piglet from core groups of five provinces. The accuracy for the strongest relevant sign to judge susceptible / resistant individual is 97% (P=1.59*10-21). The invention provides new method for breeding for disease resistance for boar ETEC F4ac diarrheal disease.

Description

Technical field [0001] The present invention relates to the field of animal genetic breeding, in particular to a key marker site of MUC13 gene that affects F4ac diarrhea susceptibility / resistance of piglets and its application in genetic improvement of breeding pigs. Background technique [0002] Piglet diarrhea is a common infectious disease in pig production, causing huge economic losses to the world pig industry. Enterotoxigenic Escherichia coli F4 (ETEC F4) is the most important pathogen causing diarrhea in newborn and pre-weaning piglets. Take my country and Denmark as examples. About 35% and 40% of piglet diarrhea are caused by ETEC F4 infection. In our country, the fatality rate is between 10%-30%, and in some places it is even higher than this rate, resulting in a serious decline in the slaughter rate of pigs, the quality of commercial pigs, and the loss of economic benefits. [0003] The pathogenicity of ETEC F4 depends on whether it specifically binds to receptors on th...

AI Technical Summary

Problems solved by technology

The applicant conducted research on the effect of this site in the white Duroc × Erhualian resource family constructed by the applicant, and found that this marker has no polymorphism in the ancestor animals, and the F of the resource family 2 Individuals have a well-separated ETEC F4ac adhesion phenotype, which indicates that the SNP marker is only a molecular marker in linkage disequilibrium with the causative mutation of the ETECF4ac receptor gene, and cannot explain the genetic basis of ETEC F4ac receptors in all populations

Therefore, although other research groups at home and abroad have made some progress in the identification of the ETEC F4ac receptor gene, there is still no major breakthrough. So far, no real ETEC F4ac receptor gene and its key mutation sites have been identified.

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