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Environmental microbial agent preservative

A technology for environmental microorganisms and preservatives, applied in the preservation of microorganisms and other directions, can solve the problems of low survival rate of viable bacteria, rapid degradation of pollution, degradation and purification performance, etc., and achieve the effects of simple preservation method, good preservation effect, and easy operation.

Active Publication Date: 2013-04-24
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Abstract
  • Description
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Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of low survival rate of viable bacteria and rapid decline in pollution degradation and purification performance in the preservation of environmental microbial agents, and provide an environmental microbial agent with good adaptability, low cost, high preservation efficiency and simple process for room temperature storage technology to ensure that the environmental microbial agent products have more viable bacteria and have good vitality and viability when they are applied

Method used

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  • Environmental microbial agent preservative
  • Environmental microbial agent preservative
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Examples

Experimental program
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Effect test

Embodiment 1

[0011] Example 1: Refining Wastewater Treatment Liquid Microbial Inoculant Preserved at Room Temperature

[0012] The liquid microbial agent for oil refinery wastewater treatment refers to the patent "A microbial agent for oil refinery wastewater treatment, its preparation method and its application" (Patent No.: ZL 200410081504.7) by fermentation and cultivation into a liquid microbial agent, and then the preservation of different bacterial agents The components of the agent were formulated into mother liquors of different concentrations, added to the liquid bacterial agent according to the formula content, and stored at room temperature. The MPN method (maximum probable number method) was used to count the viable bacteria of the bacterial agent at different storage stages, and the preservation process was investigated. The status of medium-contaminated bacteria and the treatment effect on oil refinery wastewater.

[0013] (1) Bacteria preservative formula

[0014] Bacteria ...

Embodiment 2

[0029] Example 2: Preservation formulas at different concentrations store phenol-degrading functional bacterial agents at room temperature

[0030] Taking the patented strain 10-4 phenol-degrading high-efficiency bacteria (strain preservation number: CGMCCNo: 1268) as the research object, the phenol-containing inorganic salt medium (Na 2 HPO 4 2g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O 0.3g, trace element solution 5mL, 1000ml distilled water, pH 7.0), refer to the patent "A microbial agent for treating oil refinery wastewater, its preparation method and its application" (Patent No.: ZL 200410081504.7) to expand the cultivation into Bacterial solution, after centrifugation at 5000rpm, suspend with inorganic salt medium to make ready-to-use bacterial solution (OD.600 is 1.0), save according to the formula in Table 5, and save formula 1-1, 1-2, 1-3 Deoxidizer is ascorbic acid and copper sulfate, the inhibitor is copper sulfate, and the protective agent is glycerin; the deoxidizers o...

Embodiment 3

[0037] Embodiment 3: room temperature preserves aniline degrading functional bacterial agent

[0038] Taking the patented strain AN-P1 high-efficiency aniline-degrading bacteria (strain preservation number: CGMCC No.2783) as the research object, aniline-containing inorganic salt medium (Na 2 HPO 4 2g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O 0.3g, trace element solution 5mL, aniline 1g, 1000ml distilled water, pH 7.0), refer to the invention patent "A high-efficiency aniline-degrading bacteria and its use and use method" (patent application number: 200910058557.x) fermentation expansion Cultivate into bacterial liquid, centrifuge at 5000rpm and suspend with inorganic salt medium to make ready-to-use bacterial liquid (OD.600 is 1.0). The numbers are No. 1 and No. 2, and the formula saved in No. 1 is 0.17‰C 6 h 8 o 6 +0.025‰CuSO 4 +10‰glycerin; No. 2 preservation formula is 0.4‰Na 2 S 2 o 4 +0.2‰NaHCO 3 +2‰aniline+10‰glycerol (glycerol is volume percentage, the rest is mass p...

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Abstract

The invention belongs to the technical field of environmental protection, which specifically relates to an environmental microbial agent preservative, comprising a deoxidizing agent, an inhibitor and a preservative. The deoxidizing agent consists of ascorbic acid with copper sulfate or sodium thiosulfate with sodium bicarbonate. The inhibitor consists of copper sulfate or sodium benzoate or target characteristic pollutants. The preservative consists of glycerol. The preservative of the invention has the advantages of high economic efficiency, convenient use, long storage time, stable microbial agent performance after storage, lasting strain vigor and no secondary pollution and the like.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and specifically relates to a method for storing viable bacteria in microbial agents (i.e., environmental microbial agents) used for environmental pollution control and protection at room temperature, so as to achieve long-term and low-cost preservation and maintenance of microbial agents The purpose of improving the number of viable bacteria and the performance of pollution purification is to realize its commercialization and industrialization. Background technique [0002] At present, my country's environmental pollution is still very serious, and various pollution control processes and methods have emerged one after another, among which biological treatment methods are the mainstream technical means. In recent years, the number of microbial agents developed for the purpose of ecological environment protection and pollution prevention The use of additives can not only greatly sh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/04
Inventor 谭周亮周后珍刘庆华李旭东
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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