Gold immune quantitative detection method and device on basis of embedded type charge coupled device (CCD) image acquisition

Abstract

The invention relates to the technical field of gold immune test strip quantitative detection, in particular to a gold immune quantitative detection method and a gold immune quantitative detection device on the basis of embedded type charge coupled device (CCD) image acquisition. The method comprises the following steps of: 1) placing a test strip into a CCD image acquisition device and beginningto time by using a timer after detecting that the test strip is placed; 2) when the timing time is up, extracting the CCD image information of the test strip and transmitting the information to a digital signal processing unit; 3) performing denoising pretreatment; 4) extracting characteristic vectors, namely extracting the color characteristic vector 1 of a color histogram and the characteristicvector 2 of a chromaticity space-like model respectively; 5) preliminarily judging whether the detection is effective or not; and 6) fitting a concentration model by using the characteristic parameters as the input end of a machine learning fitting algorithm, and calculating and outputting the related quantitative analysis result. By the method and the device, the detection precision of gold immune test strip concentration quantitative detection is improved. The method and the device are easy to operate and good in using effect.

Description

technical field [0001] The invention relates to the technical field of quantitative detection of gold immune test strips, in particular to a gold immune quantitative detection method and device based on embedded CCD image acquisition. Background technique [0002] Gold immunochromatographic assay (Gold Immunochromatographic assay, GICA) is a solid phase labeling immunoassay technology that combines colloidal gold labeling technology, immunoassay technology and chromatographic analysis technology. Compared with isotope, fluorescein, chemiluminescence and other labeling technologies, gold labeling technology has unique advantages such as high detection efficiency, simple method, no pollution, stable reagents, and suitable for single-person determination. In recent years, this technology has developed rapidly. The range of applications is expanding day by day, covering almost all aspects of immunological diagnostic testing. [0003] Currently, gold immunochromatography assay i...

AI Technical Summary

Problems solved by technology

[0004] Since the current gold immune test strips mainly rely on the naked eye to interpret according to the depth of the test strip after coloring, a quantitative result cannot be obtained

Therefore, in some qualitative tests of immunochromatography, such as the interpretation of drugs, the naked eye is used for interpretation. When the color of the test strip is blurred and the noise signal is relatively large, it is easy to misjudge or miss the judgment, causing unnecessary disputes.

However, in some quantitative tests, the scope of clinical application and curative effect evaluation of the test strip are limited due to the inability to quantify.

[0005] At present, the quantification of gold immunochromatography mainly adopts two methods: photoelectric reflection and image acquisition. The main disadvantages of the photoelectric reflection method are: (1) The photoelectric reflection optical system is more complicated and the adjustment is more troublesome; (2) The placement of the test strip for detection strict requirements

In the image acquisition type gold immunoassay, the device is light and easy to adjust, but at present, the gray value of the image feature selection is mostly used for data algorithm analysis, and the detection accuracy is not as good as that of the photoelectric reflection type.

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