Preparation method of microbial oil

A microbial oil and oil-producing microorganism technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of prolonged fermentation cycle and reduced efficiency, and achieve the effects of overcoming inhibition, improving efficiency, and saving cultivated land

Inactive Publication Date: 2011-11-23
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This leads to a lag period in the growth of microorganisms and production of products, a phenomenon known as secondary growth, which results in prolonged fermentation cycles and reduced efficiency

Method used

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  • Preparation method of microbial oil

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1) Mix glucose and xylose into a mixed sugar solution, glucose 47g / L, xylose 23g / L, add 1.0g / L yeast powder, 0.1g / L ammonium chloride, 1.0g / L magnesium chloride, 0.1g / L Sodium sulfate, 11.8g / L potassium dihydrogen phosphate, 3.7g / L dipotassium hydrogen phosphate, trace element solution 1% (v / v), the balance is water, pH 6.0, and the obtained medium is sterilized at 121°C for 15 minutes for later use ;

[0024]2) Oleaginous yeast Trichosporon cutaneum AS 2.571 (purchased from China Common Microorganism Culture Collection Management Center) was cultured in a liquid seed medium at 30°C and 200 rpm for 20 hours with shaking;

[0025] 3) Introduce the oleaginous microorganism seed solution prepared in step (2) into the medium described in step (1), inoculum size 10% (v / v), and aerobically culture at 30° C. for 120 h;

[0026] 4) Terminate the fermentation. At this time, the residual glucose and xylose concentrations in the fermentation broth are respectively 0.1g / L and 1.9g...

Embodiment 2

[0028] 1) Mix glucose and xylose into a mixed sugar solution, glucose 10g / L, xylose 10g / L, add 2g / L yeast powder, 0.5g / L ammonium chloride, 0.2g / L magnesium chloride, 0.1g / L sulfuric acid Sodium, 0.4g / L potassium dihydrogen phosphate, 0.1g / L dipotassium hydrogen phosphate, trace element solution 1% (v / v), pH 5.0, the obtained culture medium was sterilized at 121°C for 15 minutes before use;

[0029] 2) Oleaginous yeast Rhodosporidium toruloides AS 2.1389 (purchased from China General Microorganism Culture Collection Management Center) was cultured in a liquid seed medium at 30°C and 200 rpm for 18 hours with shaking;

[0030] 3) Introducing the oleaginous microorganism seed solution prepared in step (2) into the culture medium described in step (1), with an inoculum size of 2% (v / v), and culturing with aeration at 25°C for 60 hours;

[0031] 4) Terminate the fermentation. At this time, the residual glucose and xylose concentrations in the fermentation broth were 0.1g / L and 1.2...

Embodiment 3

[0033] 1) Mix glucose and xylose into a mixed sugar solution, glucose 10g / L, xylose 50g / L, add 0.7g / L yeast powder, 0.2g / L ammonium chloride, 1.1g / L magnesium chloride, 0.5g / L Sodium sulfate, 8g / L potassium dihydrogen phosphate, 2g / L dipotassium hydrogen phosphate, 1% (v / v) trace element solution, pH 6.5, the obtained culture medium was sterilized at 121°C for 15 minutes for later use;

[0034] 2) Oleaginous yeast Cryptococcus curvatus ATCC 20509 (purchased from the American Type Culture Collection) was cultured in a liquid seed medium at 30° C. and 200 rpm for 24 hours with shaking;

[0035] 3) Introducing the oleaginous microorganism seed solution prepared in step (2) into the medium described in step (1), with an inoculum size of 5% (v / v), and aerated culture at 28°C for 144 hours;

[0036] 4) termination of fermentation, now residual glucose and xylose concentrations in the fermented liquid were 0g / L, 6.6g / L; carry out post-treatment according to the method of Example 1, f...

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Abstract

The invention discloses a method for preparing microbial oil. The method comprises the following steps of: performing aerated culturing on a carbon source which contains glucose and xylose and serves as a raw material to obtain a leavening; and extracting the microbial oil from the leavening. According to the method, glucose and xylose in a culture medium can be transformed synchronously, so that the inhibition function of the glucose on the utilization of other substrates during the utilization of mixed sugar is relieved, the phenomenon of secondary growth in the process is avoided, the substrate transformation rate is high, and the production cost can be lowered remarkably. The method has a great application prospect in the aspect of the production of microbial oil with a lignocellulose material.

Description

technical field [0001] The invention belongs to the production of microbial oil, in particular to a method for preparing microbial oil by utilizing oil-producing microorganisms to convert carbon sources containing glucose and xylose. Background technique [0002] Microbial oils, also known as single-cell oils, are microorganisms such as yeast, mold, bacteria, and algae that convert carbohydrates into oils and accumulate in cells under certain conditions. Any microorganism that can accumulate oil in cells exceeding 20% ​​by weight of the dry weight of the cell is called an oleaginous microorganism. According to reports, some microorganisms accumulate oil to reach more than 70% of the dry weight of the cell (Li Yonghong, Liu Bo, Zhao Zongbao, Bai Fengwu. Research on the optimization of the fermentation medium and fermentation conditions of Rhodosporidium toruloides for oil production. Chinese Journal of Bioengineering, 2006, 22 (4): 650-656). Compared with the production of ...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12R1/645
Inventor 赵宗保胡翠敏
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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