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Pseudoxanthomonasjaponensis and microorganism microbial inoculum as well as applications thereof

A microbial agent, pseudoxanthomonas technology, applied in the direction of microorganism-based methods, microorganisms, biological water/sewage treatment, etc., can solve the problem of low degradation ability

Inactive Publication Date: 2013-11-06
INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the shortcomings of existing microbial strains with low degradation ability to petroleum hydrocarbons, to provide a microbial strain capable of efficiently degrading petroleum hydrocarbons and related microbial bacterial agents and applications

Method used

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  • Pseudoxanthomonasjaponensis and microorganism microbial inoculum as well as applications thereof
  • Pseudoxanthomonasjaponensis and microorganism microbial inoculum as well as applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Enrichment and separation of petroleum hydrocarbon degrading strains

[0040] Weigh 5g of petroleum-contaminated soil mixture into a 250mL Erlenmeyer flask containing 100mL of enrichment medium, culture at 28℃, 180r / min constant temperature shaker, 5 days later, transfer 5mL of culture medium to fresh enrichment culture Base, cultivated under the same conditions for 5 days, and continuously enriched for 25 days. The culture solution was diluted by gradient and spread on solid PDA medium and cultivated at 28℃; when the plate grew, single colonies of different shapes were picked. The streak separation method is used to repeat the separation and purification until a pure culture of a single strain is obtained, and the purified strain is stored on the slant of beef extract peptone medium.

[0041] 2. Screening of strains with high degradation activity

[0042] The purified strains were inoculated into 250mL Erlenmeyer flasks containing 100mL selection medium, cultured for 5 da...

Embodiment 2

[0046] According to the experimental content and experimental methods recorded in the "Manual for Identification of Common Bacterial Systems" [4] and "Principles, Methods and Practices of Actinomycetes Systematics" [5], the strains with the preservation number of CGMCC No. 4797 were screened. Strain identification.

[0047] 1. Morphological analysis

[0048] Morphological analysis of the strain with the deposit number CGMCC No. 4797 and observation under an optical microscope, the results are as follows: figure 1 with 2 Shown.

[0049] The colony morphology was observed on solid TSA medium. After streaking the strains, the colony was cultured at a constant temperature of 28°C for 24 hours to observe the colony morphology. The colony of the strain with the deposit number of CGMCC No. 4797 is light yellow, round, smooth in texture and raised on the surface. Pick and culture the lawn for 24 hours and observe it under a 100-fold oil microscope with an optical microscope. It can be cl...

Embodiment 3

[0061] Weigh 10g of petroleum-contaminated soil mixture (oil content of 1.25g) and add 100mL beef extract peptone medium (preservation number CGMCC No. 4797 Pseudoxanthomonas japonicus. The inoculation amount is the total activity per gram of medium. The number of bacteria is 0.5×10 10 In a 250mL Erlenmeyer flask, cultured at 28°C and 180r / min in a constant temperature shaker. After 3 days, the oil content in the oil-contaminated soil mixture was detected. The result was 0.49 g. The degradation rate of the strain to oil was about 60.8 %.

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Abstract

The present invention provides a kind of Pseudoxanthomonas japonensis (Pseudoxanthomonas japonensis) with preservation number CGMCCNo. Application of bacteria agent in degradation of petroleum hydrocarbons, application in bioremediation of oil pollution, application in bioremediation of oil-contaminated soil, and application in bioremediation of oil-contaminated water bodies. The preservation number provided by the present invention is Pseudoxanthomonas japonensis (Pseudoxanthomonas japonensis) with the preservation number CGMCCNo.4797 and the microbial agent containing the bacterium has a strong degradation ability to petroleum hydrocarbons, and can be widely used in the degradation of petroleum hydrocarbons and the treatment of petroleum pollution. The remediation of oil-contaminated soil and oil-contaminated water bodies, etc.

Description

technical field [0001] The present invention relates to a kind of pseudoxanthomonas japonicum ( Pseudoxanthomonas japonensis ), and a species containing the Pseudomonas japonicum ( Pseudoxanthomonas japonensis ) microbial agents as active ingredients and their application; more precisely, it involves a kind of Pseudomonas japonicus ( Pseudoxanthomonas japonensis ), and the microbial agent containing this Pseudomonas japonicum, also relates to the application of said Pseudomonas japonicus or microbial agent in the degradation of petroleum hydrocarbons, the application in bioremediation of oil pollution, the application in bioremediation Application in oil-contaminated soil, and application in bioremediation of oil-contaminated water bodies. Background technique [0002] In today's world, petroleum has become one of the most important energy sources for human beings. A large amount of petroleum and its processed products enter the soil, causing petroleum pollution in the soi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02B09C1/10C02F3/34C12R1/01A62D101/20
Inventor 郭萍朱昌雄田云龙刘雪李峰叶婧
Owner INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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