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Microbial agent, and its preparation method and application

A microbial agent and bioremediation technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of low degradation ability

Active Publication Date: 2013-11-06
宁夏中微泰克生物技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the shortcomings of the existing microbial agents that have low degradation ability to petroleum hydrocarbons, to provide a microbial agent capable of efficiently degrading petroleum hydrocarbons and its preparation method and application

Method used

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  • Microbial agent, and its preparation method and application
  • Microbial agent, and its preparation method and application
  • Microbial agent, and its preparation method and application

Examples

Experimental program
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preparation example Construction

[0037] The present invention also provides a method for preparing the microbial agent, wherein the method comprises selecting a pseudoxanthomonas japonensis with a deposit number of CGMCC No. 4797 and a pseudoxanthomonas japonensis with a deposit number of CGMCC No. 4793. Pseudomonas sp, Gordonia amicalis with the deposit number CGMCC No. 4794, Rhodococcus ruber with the deposit number CGMCC No. 4795, and food with the deposit number CGMCC No. 4796 At least two strains of Gordonia alkanivorans are inoculated into a medium for cultivation.

[0038] In a preferred embodiment, the preparation method includes: inoculating Pseudoxanthomonas japonensis with a deposit number of CGMCC No. 4797 and at least one selected from the following strains in a culture medium. Culture: Pseudomonas sp with the deposit number CGMCC No. 4793, Gordonia amicalis with the deposit number CGMCC No. 4794, Rhodococcus ruber with the deposit number CGMCC No. 4795 ) And Gordonia alkanivorans with the deposit ...

Embodiment 1

[0065] 1. Enrichment and separation of petroleum hydrocarbon degrading strains

[0066] Weigh 5g of petroleum-contaminated soil mixture into a 250mL Erlenmeyer flask containing 100mL of enrichment medium, culture at 28℃, 180r / min constant temperature shaker, and transfer 5mL of culture medium to fresh enrichment culture after 5 days Base, cultivate under the same conditions for 5 days, and then continuously enrich the culture for 25 days. The culture solution is gradually diluted and spread on the solid PDA medium and cultivated at 28°C; after the plate has grown, pick out single colonies of different forms The streak separation method is used to repeat the separation and purification until a pure culture of a single strain is obtained, and the purified strain is stored on the slant of beef extract peptone medium.

[0067] 2. Screening of strains with high degradation activity

[0068] The above purified strains were respectively inoculated into 250 mL Erlenmeyer flasks containing 1...

Embodiment 2

[0072] According to the experimental content and experimental methods recorded in the "Manual for Identification of Common Bacteria Systems" [4] and "Principles, Methods and Practices of Actinomycetes Systematics" [5], the sieved deposit numbers are CGMCC No. 4793 and CGMCC respectively. No. 4794, CGMCC No. 4795, CGMCC No. 4796 and CGMCC No. 4797 strains were identified.

[0073] 1. Morphological analysis

[0074] Morphological analysis of the strains with deposit numbers of CGMCC No. 4793, CGMCC No. 4794, CGMCC No. 4795, CGMCC No. 4796 and CGMCC No. 4797 were carried out and observed under an optical microscope. The results were as follows: Figure 1-16 Shown.

[0075] The colony morphology was observed on solid TSA medium. After streaking the strains, the colony was cultured at a constant temperature of 28°C for 24 hours to observe the colony morphology.

[0076] The colony of the strain with the deposit number of CGMCC No. 4793 is colorless and translucent, round, with irregular e...

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Abstract

The invention provides a microbial agent which comprises a medium and thalli, wherein, the thalli comprise at least two selected from the group consisting of pseudoxanthomonas japonensis with an accession number CGMCC No.4797, pseudomonas with an accession number CGMCC No.4793, gordonia amicalis with an accession number CGMCC No.4794, rhodococcus rubber with an accession number CGMCC No. 4795 and gordonia alkanivorans with an accession number CGMCC No.4796. The invention also provides a preparation method for the microbial agent and application of the microbial agent in degradation of petroleum hydrocarbon, bioremediation of soil polluted by petroleum and bioremediation of water bodies polluted by petroleum.

Description

Technical field [0001] The invention relates to a microbial inoculum and a preparation method and application thereof; more specifically, to a microbial inoculum and a preparation method thereof, and also to the application of the biological inoculum in the degradation of petroleum hydrocarbons and the bioremediation of petroleum Application in pollution, application in bioremediation of oil-contaminated soil, and application in bioremediation of oil-contaminated water. Background technique [0002] In today's world, oil has become one of the most important energy sources for mankind. A large amount of oil and its processed products enter the soil to cause oil pollution of the soil, bring harm to mankind and the entire biosphere, and thus become a worldwide environmental problem. In the research on oil pollution, bioremediation as a potential high-efficiency and low-cost clean technology is receiving increasing attention. [0003] Bioremediation refers to a controlled or spontaneo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02B09C1/10C02F3/34C12R1/01C12R1/38A62D101/20C02F101/32
Inventor 郭萍朱昌雄田云龙刘雪李峰叶婧
Owner 宁夏中微泰克生物技术有限责任公司
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