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Microbial agent and preparation method and application thereof

A microbial agent and bioremediation technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as low degradation ability

Active Publication Date: 2013-07-10
宁夏中微泰克生物技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the shortcomings of the existing microbial agents that have low degradation ability to petroleum hydrocarbons, to provide a microbial agent capable of efficiently degrading petroleum hydrocarbons and its preparation method and application

Method used

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  • Microbial agent and preparation method and application thereof
  • Microbial agent and preparation method and application thereof
  • Microbial agent and preparation method and application thereof

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preparation example Construction

[0035] The present invention also provides a method for preparing the microbial agent, wherein the method includes Pseudoxanthomonas japonensis with the deposit number CGMCC No. 4797 and Pseudoxanthomonas japonensis with the deposit number CGMCC No. 4793 Pseudomonas sp, Gordonia amicalis with the deposit number of CGMCC No. 4794, Rhodococcus ruber with the deposit number of CGMCC No. 4795, and alkane Gordon with the deposit number CGMCC No. 4796 Gordonia alkanivorans (Gordonia alkanivorans) is inoculated into the culture medium for cultivation.

[0036] According to the present invention, the culture method includes: culturing Pseudoxanthomonas japonensis with the deposit number CGMCC No. 4797 and Pseudoxanthomonas japonensis with the deposit number CGMCC No. 4793 in separate culture systems. Pseudomonas sp, Gordonia amicalis (Gordonia amicalis) with the deposit number of CGMCC No. 4794, Rhodococcus ruber with the deposit number of CGMCC No. 4795, and alkane-eating hydrocarbons w...

Embodiment 1

[0062] 1. Enrichment and separation of petroleum hydrocarbon degrading strains

[0063] Weigh 5g of petroleum-contaminated soil mixture into a 250mL Erlenmeyer flask containing 100mL of enrichment medium, culture at 28℃, 180r / min constant temperature shaker, and transfer 5mL of culture medium to fresh enrichment culture after 5 days Base, cultivated under the same conditions for 5 days, and continuously enriched for 25 days. The culture solution was diluted by gradient and spread on solid PDA medium and cultivated at 28℃; when the plate grew, single colonies of different shapes were picked. The streak separation method is used to repeat the separation and purification until a pure culture of a single strain is obtained, and the purified strain is stored on the slant of beef extract peptone medium.

[0064] 2. Screening of strains with high degradation activity

[0065] The purified strains were inoculated into 250mL Erlenmeyer flasks containing 100mL selection medium, cultured for 5...

Embodiment 2

[0069] According to the experimental contents and experimental methods recorded in the "Manual for Identification of Common Bacteria Systems" [4] and "Principles, Methods and Practices of Actinomycetes Systematics" [5], the sieved deposit numbers are CGMCC No. 4793 and CGMCC respectively. No. 4794, CGMCC No. 4795, CGMCC No. 4796 and CGMCC No. 4797 strains were identified.

[0070] 1. Morphological analysis

[0071] Morphological analysis of the strains with deposit numbers of CGMCC No. 4793, CGMCC No. 4794, CGMCC No. 4795, CGMCC No. 4796 and CGMCC No. 4797 were carried out and observed under an optical microscope. The results were as follows: Figure 1-16 Shown.

[0072] The colony morphology was observed on solid TSA medium. After the strain was streaked inoculated, the colony morphology was observed by culturing at 28°C for 24 hours.

[0073] The colony of the strain with the deposit number of CGMCC No. 4793 is colorless and translucent, round, with irregular edges, smooth texture a...

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Abstract

The invention provides a microbial agent, which comprises a culture medium and a thallus, wherein the thallus comprises psesudoxanthomonasjaponensis with the collection number CGMCC No.4797, pseudomonas with the collection number CGMCC No.4793, gordoniaamicalis with the collection number CGMCC No.4794, phodococcusruber with the collection number CGMCC No.4795 and gordoniaalkanivorans with the collection number CGMCC No.4796. The invention further provides a preparation method of the microbial agent, application of the microbial agent to degradation of petroleum hydrocarbon, application of the microbial agent to biological repair of petroleum pollution, application of the microbial agent to biological repair of soil suffering from petroleum pollution and application of the microbial agent to biological repair of water bodies suffering from petroleum pollution.

Description

Technical field [0001] The invention relates to a microbial inoculum and a preparation method and application thereof; more specifically, to a microbial inoculum and a preparation method thereof, and also to the application of the biological inoculum in the degradation of petroleum hydrocarbons and the bioremediation of petroleum Application in pollution, application in bioremediation of oil-contaminated soil, and application in bioremediation of oil-contaminated water. Background technique [0002] In today's world, oil has become one of the most important energy sources for mankind. A large amount of oil and its processed products enter the soil to cause oil pollution of the soil, bring harm to mankind and the entire biosphere, and thus become a worldwide environmental problem. In the research on oil pollution, bioremediation as a potential high-efficiency and low-cost clean technology is receiving increasing attention. [0003] Bioremediation refers to a controlled or spontaneo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00C12N1/20A62D3/02B09C1/10C02F3/34C12R1/01C12R1/38A62D101/20
Inventor 郭萍朱昌雄田云龙刘雪李峰叶婧
Owner 宁夏中微泰克生物技术有限责任公司
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