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Rhodococcusruber and microbial agent and application of Rhodococcusruber and microbial agent

A microbial agent, rhodococcus technology, applied in the direction of microorganism-based methods, microorganisms, biological water/sewage treatment, etc., can solve the problem of low degradation ability

Inactive Publication Date: 2013-11-06
INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the shortcomings of existing microbial strains with low degradation ability to petroleum hydrocarbons, to provide a microbial strain capable of efficiently degrading petroleum hydrocarbons and related microbial bacterial agents and applications

Method used

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  • Rhodococcusruber and microbial agent and application of Rhodococcusruber and microbial agent
  • Rhodococcusruber and microbial agent and application of Rhodococcusruber and microbial agent
  • Rhodococcusruber and microbial agent and application of Rhodococcusruber and microbial agent

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Experimental program
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Effect test

Embodiment 1

[0041] 1. Enrichment and isolation of petroleum hydrocarbon degrading bacterial strains

[0042] Weigh 5g of the oil-contaminated soil mixture and add it to a 250mL Erlenmeyer flask filled with 100mL enrichment medium, cultivate it at 28°C and 180r / min on a constant temperature shaker, and transfer 5mL of the culture medium to a fresh enrichment culture after 5 days base, cultured under the same conditions for 5 days, and continuously enriched and cultured for 25 days in this way, the culture solution was diluted serially and spread on solid PDA medium, and cultured at 28°C; after colonies grew on the plate, single colonies of different shapes were picked Separation and purification were carried out repeatedly by streak separation until a pure culture of a single strain was obtained, and the purified strain was preserved in the slant of beef extract peptone medium.

[0043] 2. Screening of strains with high degradation activity

[0044] Inoculate the above-mentioned purified ...

Embodiment 2

[0048] According to the experimental content and experimental methods recorded in the "Common Bacteria System Identification Manual" [4] and "Actinomycete Systematic Principles, Methods and Practice" [5], the strains with the preservation number of CGMCC No. 4795 were screened. Bacteria identification.

[0049] 1. Morphological analysis

[0050] Morphological analysis was carried out on the strain with the preservation number CGMCC No. 4795 and observed under an optical microscope and a scanning electron microscope. The results are as follows: Figure 1-4 shown.

[0051] The observation of the colony morphology was carried out on solid TSA medium. After the strain was streaked and inoculated, it was cultured at a constant temperature of 28°C for 24-48 hours to observe the colony morphology. The colonies of the strain with the preservation number CGMCC No. 4795 are pink, round, slightly rough in texture and raised on the surface. After picking and culturing for 24 hours, the...

Embodiment 3

[0063] Weigh 10g of petroleum-contaminated soil mixture (1.25g of petroleum content) and add it to 100mL of beef extract peptone medium (preservation number is CGMCC No. 4795). ×10 10 1) in a 250mL Erlenmeyer flask, cultured at 28°C and 180r / min on a constant temperature shaker. After 3 days, the oil content in the oil-contaminated soil mixture was detected, and the result was 0.35 grams. The degradation rate of the strain to oil was about 72 %.

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Abstract

The invention provides a Rhodococcus bacterium (Rhodococcus ruber) with a preservation number of CGMCCNo.4795, and a microbial agent containing the Gordonella, and also provides the effectiveness of the Gordonella or the microbial agent in degrading petroleum hydrocarbons. Applications, applications in bioremediation of oil-contaminated soils, applications in bioremediation of oil-contaminated soils, and applications in bioremediation of oil-contaminated water bodies. The Rhodococcus ruber (Rhodococcus ruber) with the preservation number CGMCCNo.4795 provided by the present invention and the microbial agent containing the bacterium have a strong degradation ability to petroleum hydrocarbons, and can be widely used in the degradation of petroleum hydrocarbons and the restoration of petroleum pollution, such as Remediation of oil-contaminated soil and remediation of oil-contaminated water bodies, etc.

Description

technical field [0001] The present invention relates to a kind of rhodococcus erythrococcus ( Rhodococcus ruber ), and a species containing the Rhodococcus ( Rhodococcus ruber ) microbial agents as active ingredients and their application; more precisely, it involves a kind of Rhodococcus rubrum ( Rhodococcus ruber ), and the microbial agent containing this Rhodococcus, also relates to the application of said Rhodococcus or microbial agent in the degradation of petroleum hydrocarbons, the application in bioremediation of oil pollution, the application in bioremediation of oil-contaminated soil, And its application in bioremediation of oil-contaminated water bodies. Background technique [0002] In today's world, petroleum has become one of the most important energy sources for human beings. A large amount of petroleum and its processed products enter the soil, causing petroleum pollution in the soil, which brings harm to human beings and even the entire biosphere, thus bec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02C02F3/34B09C1/10C12R1/01A62D101/20C02F103/34
Inventor 郭萍田云龙崔丽虹朱昌雄刘雪李峰叶婧
Owner INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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