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Application of CCFL (cold cathode fluorescent lamp) in plant tissue culture

A plant tissue culture and culture rack technology, applied in the field of new light sources, can solve problems such as no discovery, and achieve the effects of long life, improved energy utilization, and reduced cost

Inactive Publication Date: 2011-11-23
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no application of CCFL as a light source for plant tissue culture has been found so far.

Method used

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  • Application of CCFL (cold cathode fluorescent lamp) in plant tissue culture
  • Application of CCFL (cold cathode fluorescent lamp) in plant tissue culture
  • Application of CCFL (cold cathode fluorescent lamp) in plant tissue culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Hemerocallis "golden baby" was used in the experiment, and a small plant with 2 leaves was used as the test material. The ratio of CCFL red / blue light is 6:4; 7:3; 8:2 respectively, and ordinary fluorescent lamp is used as the control. Use MS + sucrose 30g / L + agar 6g / L as the medium, and adjust the pH value to 5.8. A 100ml Erlenmeyer flask was used as a culture vessel. The culture temperature is 25°C, the light time is 24h / d, and the light intensity is 3500 lux. The test materials were inoculated with 3 plants per container, and 10 containers were inoculated with each of the four treatments of each variety, a total of 30 plants. The actual measured number of plants for each treatment was 15. Repeat the test twice.

[0024] Determination of growth index: the culture time of each treatment is 30 days. Test the growth indicators after 30 days of cultivation in the tube and 30 days after transplanting, including plant height, number of leaves, root number, maximum roo...

Embodiment 2

[0034] The experiment uses the iris "Iris" as the experimental material. Take the plantlet with 2 leaves as the test material. The ratio of CCFL red / blue light is 6:4; 7:3; 8:2, respectively, and the fluorescent lamp is used as the control. Use MS + sucrose 30g / L + agar 6g / L as the medium, and adjust the pH value to 5.8. A 100ml Erlenmeyer flask was used as a culture vessel. The culture temperature is 25°C, the light time is 24h / d, and the light intensity is 3500 lux. The test materials were inoculated with 3 plants per container, and 10 containers were inoculated with each of the four treatments of each variety, a total of 30 plants. The actual measured number of plants for each treatment was 15. Repeat the test twice.

[0035] Determination of growth index: the culture time of each treatment is 30 days. Test the growth indicators after 30 days of cultivation in the tube and 30 days after transplanting, including plant height, number of leaves, root number, maximum root...

Embodiment 3

[0056] Embodiment 3CCFL culture frame

[0057] Such as image 3 As shown, the CCFL cultivation frame includes a frame body 2, and the frame body is provided with a plurality of partitions 3 to form a multi-layer pattern, and the bottom surface of the partition is provided with several CCFL lamp tubes 4 ( figure 2 ), the lamp tube 4 is connected to the controller 1. The controller 1 is used to control and adjust the light intensity of each CCFL.

[0058] CCFLs are arranged in an orderly manner. Considering the adjustment and use of red and blue light, it can be arranged according to the 7:3 ratio of red light and blue light tubes during installation. When in use, part of the red or blue light tubes can be opened or closed according to needs, and the ratio of red and blue light can be adjusted. By adjusting the light intensity, the light source can be optimized.

[0059] The above partitions are detachable, and their heights are adjustable, and the distance between layers c...

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Abstract

The invention provides an application of a CCFL (cold cathode fluorescent lamp) in plant tissue culture, in particular an application of CCFL used as the light source of plant tissue culture. In the invention, researches are performed to further determine that the utilization rate of the light source can be increased by adopting a special proportion of red light and blue light and the increment of plant can be effectively increased by adopting a special illumination intensity. The CCFL used as the light source can be widely used in plant tissue culture so as to increase the growth efficiency of plant and reduce the energy consumption.

Description

technical field [0001] The invention relates to a novel light source for plant tissue culture. Background technique [0002] Plant tissue culture is based on the theory that plant cells are totipotent, using isolated organs, tissues or cells of plants, such as roots, stems, leaves, flowers, fruits, seeds, embryos, ovules, ovaries, anthers, pollen and The parenchyma and vascular tissue of storage organs can induce callus, adventitious buds and adventitious roots under sterile and suitable artificial medium, light, temperature and other conditions, and finally form a complete plant. So far, tissue culture technology has been widely used in clone reproduction, flower breeding, plant virus removal and germplasm preservation all over the world. [0003] The light sources used in plant tissue culture are usually ordinary fluorescent lamps and high pressure sodium lamps. They generally have the disadvantages of short life, high heat generation and unsatisfactory luminous efficien...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 高亦珂李萌张启翔程堂仁
Owner BEIJING FORESTRY UNIVERSITY
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