Primer library and screening system for rapid PCR (Polymerase Chain Reaction) detection for population sudden viral epidemics

Abstract

The invention relates to a primer library and screening system for rapid PCR (Polymerase Chain Reaction) detection for population sudden viral epidemics. The invention relates to a viral primer design and assessment system and belongs to the field of combination of technologies of biology and computer databases. The primer library and screening system mainly comprises a clinical virology database module, a virus sequence database module, a virus sequence automatic extraction module, a virus variation sequence function prediction module, a virus species specificity sequence and virus subtype specificity sequence screening module, a primer generation and evaluation module and an amplification product interpretation module. The invention aims to achieve the following purposes: firstly, an optimal PCR-amplified virus primer sequence is designed according to a target gene sequence; secondly, the specificity and accuracy of the primer are ensured; thirdly, the matching degree between an amplified fragment and a target virus is predicted under the condition that sequencing is not carried out; and fourthly, the clinical background data and referenced therapeutic schedules of a virus are predicted according to the amplified sequence of the PCR primer.

Description

technical field [0001] The invention is a virus primer design and evaluation system, which belongs to the field of combination of biology and computer database technology. Background technique [0002] Polymerase Chain Reaction (Polymerase Chain Reaction, PCR) is a method for rapidly amplifying DNA in vitro, used to amplify specific DNA fragments, within a few hours, the target gene fragment can be amplified to millions of copies of molecular biology learn technology. The basic principle of PCR technology is similar to the natural replication process of DNA, and its specificity depends on oligonucleotide primers complementary to both ends of the target sequence. PCR consists of three basic reaction steps: denaturation-annealing-extension: ① Denaturation of template DNA: After the template DNA is heated to about 93°C for a certain period of time, the double-stranded DNA of the template or the double-stranded DNA formed by PCR amplification is decomposed. ②Annealing (anneali...

AI Technical Summary

Problems solved by technology

In addition, for a certain disease, there may be multiple viruses acting together to cause the disease, so it is impossible to design suitable primers to amplify the virus sequence before knowing the specific virus species, so the conventional primer design method cannot be very good. Fully meet the needs of viral primer design

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