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Single-type melanins and their preparation methods and applications

A kind of melanin, a single technology, applied to melanin and its synthesis method and the application field in medicines and cosmetics, can solve the problems of a single type of melanin preparation method and the like

Inactive Publication Date: 2011-10-26
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The problem to be solved by the present invention is to disclose a single type of melanin, its preparation method and its application in medicine and cosmetics

Method used

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  • Single-type melanins and their preparation methods and applications
  • Single-type melanins and their preparation methods and applications
  • Single-type melanins and their preparation methods and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: the preparation method of powdery DHN-melanin

[0027] 1) Slowly add 1,8-DHN to citric acid-Na 2 HPO 4 buffer (pH5.0), stirred,

[0028] 2) Continuously add 1M NaNO during stirring 2 The solution was equimolar, stirred at room temperature for half an hour,

[0029] 3) The reaction product is filtered, and the insoluble matter is rinsed twice with ethyl acetate, methanol, and water respectively,

[0030] 4) The precipitate was dried naturally, and it was confirmed to be powder DHN-melanin by EPR test, IR test, and electron microscope observation.

Embodiment 2

[0031] Embodiment 2: ammonium sulfate concentration method prepares fungus Daldinia eschscholzii IFB-TL01 extracellular crude protein

[0032] The fungus IFB-TL01 was activated on a PDA plate, inoculated fresh bacterial mass into 1 L Erlenmeyer flasks, each containing 400 mL of ME medium, inoculated 5-6 flasks on a shaker, at 150-200 rpm, After culturing at 28°C for 4 days, filter the bacteria, centrifuge the bacteria solution at 4°C, 10,000 rpm for 20 min to remove residual mycelium; slowly add ammonium sulfate to the bacteria solution to make the final concentration 80% (per liter of bacteria solution Add 516g of ammonium sulfate), stir overnight with a magnetic stirrer at 4°C to fully precipitate the protein; centrifuge at 10,000rpm at 4°C for 20 min and remove the supernatant; precipitate with an appropriate amount of citric acid-Na 2 HPO 4 Dissolve the buffer solution (filter paper to remove insoluble matter); put it in a dialysis bag for dialysis; then freeze-dry the ...

Embodiment 3

[0033] Example 3: Determination of extracellular crude protease activity of fungi IFB-TL01

[0034] Referring to the method of Robert Bourbounais, 3-ethylbenzothiazole-6-sulfonic acid (ABTS) was used as the substrate to determine the oxidation rate of the enzyme. The specific method is: the reaction is carried out at room temperature, the total reaction volume is 3 ml, including 0.5 ml 0.5 mmol / L ABTS and 1.5 ml 0.1 mmol / L citric acid, 1 ml of fermentation broth is added, and the absorbance value is measured at 420 nm. Change, the unit of the enzyme is defined as the increase in the absorbance value of the enzyme solution per minute ΔOD420 / (ml.min), which proves that the extracellular crude protein of TLO1 has laccase (or oxidase) activity.

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Abstract

The invention discloses single-type melanins, their preparation methods and their applications in drugs and cosmetics. The preparation method comprises that a dihydroxynaphthlene-melanin (DHN-melanin) in powder form is prepared through a reaction of 1,8-dihydroxynaphthlene as a raw material and NaNO2 in an acid condition, and a DHN-melanin in flake form is prepared through a catalytic action of a fungus Daldiniaeschscholzii IFB-TL01 extracellular crude protein. The melanins prepared respectively in the two forms both have strong activities of elimination of hydroxyl free radicals. The melanins can be utilized in cosmetics and have effects of oxidation and radiation resistance and hydroxyl free elimination. The melanins can be utilized in hair colorings and have adornment effects. The melanins also can be utilized in the biological insecticide field and act as photoprotectants. The melanins are suitable for the biomedical technical field comprising drugs, cosmetics and the like.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a melanin, its synthesis method and its application in medicine and cosmetics. Background technique [0002] Melanin is an amorphous small particle insoluble in water and almost all solvents. Melanin is a heterogeneous polyphenol-like polymer widely present in animals, plants and microorganisms. At present, the extraction of natural melanin mainly uses animal tissues (silkie, octopus, etc.), plants (black rice, black sesame, etc.), microorganisms (melanin-producing actinomycetes, fungi, etc.). Melanin is widely used as ultraviolet absorber, antioxidant, free radical scavenger, cation chelating agent and new natural drug carrier, and can also be used to treat some nervous system diseases related to melanin deficiency. In addition, melanin has broad application prospects in the decoration of cosmetics, hair dyes, and photoprotectants of biopesticides. [0003] As ...

Claims

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Application Information

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IPC IPC(8): C08G65/38C12P1/02A01N25/22A61K8/86A61Q5/10A61Q19/00
Inventor 谭仁祥汪伟宋雅男沈薇宋勇春
Owner NANJING UNIV
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