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Key genes, microRNAs and other non-coding RNAs or combination thereof used for identifying or regulating cell pluripotency

A non-coding, pluripotent technology, applied in the field of identifying or regulating key genes, microRNA, other non-coding RNAs or combinations thereof, which can solve the problem of inability to verify the pluripotency of human iPS cells, delay iPS Technology development and application, low success rate and other issues

Active Publication Date: 2015-01-07
INST OF ZOOLOGY CHINESE ACAD OF SCI +1
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Problems solved by technology

However, the selection of fully reprogrammed iPS cells through tetraploid embryo compensation has high technical requirements, and the success rate is very low, which seriously delays the development and application of iPS technology
Due to ethical restrictions, the pluripotency of human iPS cells cannot be verified by compensation of tetraploid embryos, which makes iPS cells face a major obstacle in the final clinical trials

Method used

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  • Key genes, microRNAs and other non-coding RNAs or combination thereof used for identifying or regulating cell pluripotency
  • Key genes, microRNAs and other non-coding RNAs or combination thereof used for identifying or regulating cell pluripotency
  • Key genes, microRNAs and other non-coding RNAs or combination thereof used for identifying or regulating cell pluripotency

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Embodiment Construction

[0023] The inventor's research is based on rigorous animal experiments, identifying and classifying embryonic stem cells and pluripotent stem cells with different developmental potentials, and tracing the differences between these pluripotent stem cells through large-scale Solexa sequencing and gene chip data, thereby It was found that genes, microRNAs and other non-coding RNAs located in the Dlk1-Dio3 segment were highly expressed in fully pluripotent cells with the highest developmental potential, but were significantly suppressed or silenced in incomplete pluripotent stem cells with lower developmental potential; through The results obtained by detecting this cluster of genes and non-coding small RNAs and then verifying pluripotency are also completely consistent with this conclusion. Thus, in mice, the expression of this cluster of genes is an important marker of stem cell pluripotency.

[0024] MicroRNA (miRNA) refers to a short chain of up to 22 nucleotides whose precurs...

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Abstract

The invention relates to key genes, microRNAs and other non-coding RNAs, or a combination thereof used for identifying or regulating cell pluripotency. The invention is characterized in that the key genes, microRNAs, other non-coding RNAs or a combination is highly expressed in the stem cell with complete pluripotency and the expression is obviously repressed or silent in the stem cell without complete pluripotency. The genes, microRNAs and other non-coding RNAs are the genes, microRNAs and other non-coding RNAs positioned in the chromosome imprinting region named as Dlk1-Dio3 on the long arm of mouse chromosome 12 and the genes, microRNAs and other non-coding RNAs in the genome collinearity regions of other mammals, which have 70%-100% of homology. The invention also relates to applications of the genes, microRNAs and other non-coding RNAs, or the combination thereof used for identifying the pluripotency of the stem cell and regulating cell pluripotency, applications in stem cell typing, applications for regulating the cell pluripotency and the pluripotency state and level of the cell, applications in disease treatment, and applications in the drug target development of tumor treatment or the development of antitumor drugs.

Description

technical field [0001] The present invention relates to key genes, microRNAs or other non-coding RNAs or combinations thereof for identifying or regulating cell pluripotency. The present invention also relates to the use of the gene and non-coding small RNA of the present invention in identifying stem cell pluripotency and in stem cell typing; in regulating cell pluripotency and regulating the state and level of cell pluripotency; in diseases Use in therapy; use in the development of drug targets for tumor treatment, or in the development of anti-tumor drugs. Background technique [0002] Pluripotent cells (pluripotent cells) refer to a class of cells with multi-directional differentiation potential that have undergone division and differentiation to form various tissues and cells of human and animal individuals. At present, embryonic stem cell (ESC) is the most well-known pluripotent cell. Embryonic stem cells are a special cell line obtained after isolation of cell mass ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 周琪王秀杰王柳刘蕾赵小阳杨维骆观正吕卓郑钦元吴华君李伟
Owner INST OF ZOOLOGY CHINESE ACAD OF SCI
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