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Application of sodium oxamate in preparing drugs for radiotherapy sensitization

The technology of sodium oxalate and drug is applied in the application field of preparing radiotherapy sensitization drugs, which can solve the problems such as no reports on radiotherapy sensitization of sodium oxamate, and achieve the effect of improving radiotherapy sensitivity.

Inactive Publication Date: 2011-10-05
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is no report on radiosensitization of sodium oxamate in the prior art

Method used

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  • Application of sodium oxamate in preparing drugs for radiotherapy sensitization
  • Application of sodium oxamate in preparing drugs for radiotherapy sensitization
  • Application of sodium oxamate in preparing drugs for radiotherapy sensitization

Examples

Experimental program
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Effect test

Embodiment 1

[0017] (1) Cell culture: The cells were cultured in DMEM medium containing 10% calf serum, glutamine and 1 million U / L penicillin and streptomycin. Cells were placed in 5% CO 2 , cultured in an incubator at 37°C, passaged once every 2-3 days, and cells in logarithmic growth phase were used for experiments.

[0018] (2) CCK-8 colorimetric method: cells in logarithmic growth phase (5×10 24 hours before drug treatment) 4 / mL) were inoculated into 96-well plates, and 6 parallel wells were set for each concentration. Different concentrations of sodium oxamate were added to continue the incubation for 12, 24 or 48 hours. Add 10 μL of CCK-8 reagent to the cell culture medium and continue to culture for 1-4 hours. The absorbance at 450 nm was measured using a microplate reader. Finally, the cell viability was calculated by the formula of cell viability=value of drug-added group / value of control group×100%.

[0019] The result is as figure 1 As shown, the results showed that sodi...

Embodiment 2

[0021] (1) cell culture is with embodiment one;

[0022] (2) Cell clone formation method: take cells in logarithmic growth phase, and inoculate different numbers of cells into 60 mm culture dishes according to different irradiation dose requirements (the number of cells inoculated at 0, 2, 4, 6, and 8 Gy doses is 400, respectively. , 2000, 6000, 1000 and 15000). After 24 hours, the combined sodium oxalate and radiation group were treated with 0.75 μmol / L sodium oxalate for 24 hours, and the control group was treated with the same amount of absolute ethanol as a negative control. The cells in the two groups received different doses (0-8Gy) of γ-ray irradiation at the same time, and continued to culture for 24 hours after the irradiation. Both groups were replaced with ordinary medium, and the culture was continued until obvious cell clones appeared in the culture dish. . The culture medium was discarded, washed twice with PBS, fixed with anhydrous methanol for 30 minutes, sta...

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Abstract

The invention which belongs to the field of drugs for radiotherapy sensitization relates to a new use of sodium oxamate, that is, an application of sodium oxamate in preparing the drugs for radiotherapy sensitization. The structural formula of sodium oxamate is shown as follows: NH2(CO)2ONa. The sodium oxamate can be used to prepare the drugs for radiotherapy sensitization, and low concentration sodium oxamate with no toxic and side effects allows the radiation sensitivity of tumor cells to be improved.

Description

technical field [0001] The invention belongs to the field of radiotherapy sensitizing drugs, and relates to a new use of sodium oxamate, that is, the application in the preparation of radiotherapy sensitizing drugs. Background technique [0002] Malignant tumor is a common and frequently-occurring disease, and it has become one of the major diseases that seriously threaten human life and health. As one of the three main methods of tumor treatment, radiotherapy has been widely and routinely used in tumor treatment. According to the statistics of major cancer prevention and treatment centers in China, about 65%-75% of diagnosed and treated cancer patients need radiotherapy, especially head and neck cancer, prostate cancer, esophageal cancer, etc. However, even in patients with nasopharyngeal carcinoma who have a good effect of radiotherapy, problems such as high local recurrence and radiation resistance still occur after radiotherapy. And just relying on simply increasing th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/194A61P35/00A61P11/02A61P11/04
Inventor 樊赛军伍理
Owner SUZHOU UNIV
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