Method for detecting 33 kinds of narcotic drugs in urine by liquid chromatography-tandem mass spectrometry
A liquid chromatography, tandem mass spectrometry technology, applied in the field of drug detection, can solve the problem of high false positive rate, achieve the effect of good overall recovery rate, saving processing time, saving organic solvent and
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0041] Example 1. A liquid chromatography-tandem mass spectrometry detection method for 33 drugs in urine, the steps are as follows:
[0042] (1) Sample treatment: Weigh 2g of the urine sample to be tested, accurate to 0.01g, put it into a 50mL centrifuge tube with stopper, add 10ml of ethyl acetate, mix well on a liquid mixer for 1min, and centrifuge at 4000r / min 10min, draw the upper layer of ethyl acetate into the test tube, then add 1mL of 0.1mol / L NaOH solution, mix thoroughly on the liquid mixer, add 10ml of ethyl acetate to repeat the extraction once, and combine the two extracts; Blow dry at 40°C with a nitrogen gas drying apparatus, add 1 mL of methanol:water with a volume ratio of 30:70, vortex, pass through a 0.25 μm filter membrane, and obtain the sample to be tested;
[0043] (2) Determination: The sample to be tested is measured with a liquid chromatography-tandem quadrupole mass spectrometer; in the liquid chromatography-tandem mass spectrometry analysis of 33 ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com