Laccase activated and modified sulfonated soda lignin amphiphilic polymer and preparation method and application thereof

A technology of amphiphilic polymer and alkali lignin, which is applied in the field of laccase-activated modified sulfonated alkali lignin amphiphilic polymer and its preparation, achieving the effects of low production cost, overcoming severe reaction conditions and mild modification conditions

Active Publication Date: 2012-09-05
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] The current modification of laccase-activated lignin is mainly used in papermaking wastewater treatment, pulp bleaching process, lignin extraction and purification, and the technology involving biological modification to improve lignin reactivity has not been reported.

Method used

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  • Laccase activated and modified sulfonated soda lignin amphiphilic polymer and preparation method and application thereof
  • Laccase activated and modified sulfonated soda lignin amphiphilic polymer and preparation method and application thereof
  • Laccase activated and modified sulfonated soda lignin amphiphilic polymer and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Dissolve 100g of wheatgrass alkali lignin (obtained from wheat straw pulping black liquor by acid analysis) in 150g of acetic acid-sodium acetate buffer solution with a pH of 4.5, raise the temperature to 60°C, add 200U laccase (1U= 1μmolABTS conversion / min) (the laccase derived from Coriolus versicolor , the enzyme activity is 100U / g. That is, the amount of laccase is 2g), and react for 2 hours to obtain activated alkali lignin; dissolve 10g of sodium sulfite in 90g of water, and adjust the pH to 9 with a 20% NaOH aqueous solution. When the temperature is controlled at 30°C, 5g of epichlorohydrin is added dropwise, and after 0.5 hours of reaction, the temperature is raised to 60°C, and the reaction is continued for 0.5 hours to obtain sodium hydroxysulfonate; mix it with activated alkali lignin, and adjust the pH to 8, The temperature was raised to 95° C., and reacted for 0.5 hours, then 20 g of formaldehyde solution with a mass concentration of 20% was added dropwise...

Embodiment 2

[0050] Dissolve 100g of bamboo pulp alkali lignin (obtained from bamboo pulp alkali pulping black liquor by acid analysis) in 900g of citric acid-sodium citrate buffer solution with a pH of 6.0, heat up to 40°C, add 2000U laccase ( The laccase is derived from Rhus vernicifera , the enzyme activity is 200U / g, that is, the amount of laccase is 10g), and react for 6 hours to obtain activated alkali lignin; dissolve 30g of sodium sulfanilate in 45g of water, and adjust it with 20% NaOH 0 aqueous solution When the pH is 12 and the temperature is controlled at 40°C, 20g of epichlorohydrin is added dropwise, and after 2 hours of reaction, the temperature is raised to 60°C, and the reaction is continued for 1 hour to obtain sodium hydroxysulfonate; mix it with activated alkali lignin, adjust The pH was 12, the temperature was raised to 95°C, and the reaction was carried out for 1 hour, then 80 g of acetaldehyde solution with a mass concentration of 37% was added dropwise, and the pol...

Embodiment 3

[0052] Dissolve 100g of bamboo pulp alkali lignin in 400g of succinic acid-sodium succinate buffer solution with a pH of 4.0, raise the temperature to 50°C, and add 1000U of fungal laccase (the laccase is derived from Trametes versicolor , the enzyme activity is 300U / g), reacted for 3 hours to obtain activated alkali lignin; dissolved 10g sodium sulfite and 15g sodium bisulfite in 60g water, adjusted the pH to 10 with 20% NaOH aqueous solution, and controlled the temperature At 40°C, add 15g of epichlorohydrin dropwise, react for 1 hour, heat up to 85°C, and continue the reaction for 0.75 hours to obtain sodium hydroxysulfonate; mix it with activated alkali lignin, adjust the pH to 12, and heat up to 85°C, reacted for 1 hour, then added dropwise 70 g of isobutyraldehyde aqueous solution with a mass concentration of 30%, and carried out polycondensation reaction for 3 hours to obtain a liquid sulfonated alkali lignin amphiphilic polymer.

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Abstract

The invention discloses a laccase activated and modified sulfonated soda lignin amphiphilic polymer and a preparation method and application thereof. The preparation method comprises the following steps of: dissolving base lignin in a buffer solution, and adding laccase to obtain laccase activated base lignin; dripping epichlorohydrin into aqueous solution of a sulfonating agent, and reacting to obtain sodium hydroxyl sulfonate; mixing the laccase activated base lignin with the sodium hydroxyl sulfonate, and dripping an aldehyde substance for polycondensation to obtain the sulfonated soda lignin amphiphilic polymer, wherein the sulfonated soda lignin amphiphilic polymer comprises 100 parts of base lignin, 10 to 30 parts of sulfonating agen, 5 to 20 parts of epichlorohydrin and 20 to 80 parts of aldehyde substance. The prepared sulfonated soda lignin amphiphilic polymer has the sulfonated degree of more than 3.00mmol / g, the weight-average molecular weight of more than 12,500, and good dispersibility for concrete and coal water slurry; and when the sulfonated soda lignin amphiphilic polymer is used for preparing concrete, the using amount of the sulfonated soda lignin amphiphilic polymer is 0.2 to 0.6 percent of the weight of cement in the concrete; and when the sulfonated soda lignin amphiphilic polymer is used for preparing the coal water slurry, the using amount of the sulfonated soda lignin amphiphilic polymer is 0.5 to 1.5 percent of the weight of coal dust.

Description

technical field [0001] The invention relates to an alkali lignin amphiphile polymer, in particular to a laccase-activated modified sulfonated alkali lignin amphiphile polymer and a preparation method thereof. Background technique [0002] In recent years, resource shortage and environmental pollution have become one of the main problems plaguing the development of human society. Lignin is a biomass resource second only to cellulose in nature, accounting for about 20-30% of the weight of plants. The world can produce about 150 billion tons of lignin every year. In the paper pulping process, lignin is dissolved in the waste liquid and becomes industrial lignin. Technical lignin mainly includes lignosulfonate from the red liquor of sulfite pulping and alkaline pulping. Alkali lignin of black liquor, alkali lignin can be converted into lignosulfonate by sulfonation. Because of its certain surface activity, lignosulfonate has been widely used as oil field surfactant, concrete a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G16/02C08H7/00C04B24/16C10L1/24C10L1/32
Inventor 邱学青杨东杰周海峰楼宏铭周明松孙勇欧阳新平
Owner SOUTH CHINA UNIV OF TECH
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