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Method for degrading pectic polysaccharides with high efficiency

A pectin polysaccharide and high-efficiency technology, applied in the biological field, can solve the problems of low degradation efficiency, complexity, low oligosaccharide yield, etc., and achieve the effect of simple treatment process

Inactive Publication Date: 2011-09-21
NORTHWEST UNIV
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  • Abstract
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Problems solved by technology

However, due to the complex structure of pectin and its large molecular weight, the microenvironment in the molecule affects the contact between pectinase and the action site, so there are certain limitations in directly degrading pectin with pectinase, the degradation efficiency is low, and the yield of oligosaccharides is low. Low, difficult to select suitable enzymes to efficiently prepare pectin oligosaccharides

Method used

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  • Method for degrading pectic polysaccharides with high efficiency
  • Method for degrading pectic polysaccharides with high efficiency
  • Method for degrading pectic polysaccharides with high efficiency

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Embodiment Construction

[0024] Below in conjunction with specific embodiment the present invention is described in further detail:

[0025] The invention provides a method for efficiently degrading pectin polysaccharides, comprising the following steps:

[0026] 1. Dissolve 2 g of pectin in 0.5 mol / L trifluoroacetic acid (TFA) at a mass concentration of 1%, and acid hydrolyze it in a water bath at 80°C for 5 hours. volume of distilled water, fully shaken, centrifuged, and the supernatant and precipitate were collected and combined to obtain two components, the supernatant and the precipitate. The supernatant is 0.591g, the precipitate is 1.142g, and the yield can reach 86.65%, wherein the content of the precipitate is about twice that of the supernatant.

[0027] The supernatant fraction was detected by fluorescence-assisted carbohydrate electrophoresis (FACE), and the electrophoresis pattern showed that the supernatant fraction was also a pectin-oligosaccharide mixture.

[0028] After pectin, pect...

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Abstract

The invention provides a method for degrading pectic polysaccharides with high efficiency, which comprises the following steps: 1) subjecting part of pectic polysaccharides to acid hydrolysis; and 2) performing enzymolysis of a product obtained by the step 1). The invention provides the method for degrading pectic polysaccharides, which have high oligogalacturonide content and high degree of polymerization, with high efficiency.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a method for degrading pectin polysaccharides by combining chemical methods and enzymatic methods, in particular to a method for degrading pectin polysaccharides into a certain biological A method for oligogalacturonic acid with an active polymerization degree of 2-11. Background technique [0002] Pectin is a kind of acid heteropolysaccharide with complex structure, mainly composed of galacturonan (HGA), rhamnogalacturonan-I (RG-I) and rhamnogalacturonan-II (RG-II) consists of three structural regions, in which HGA is a smooth region, and RG-I and RG-II are whisker-like regions. The number, type and connection mode of residues in the side chain and the presence of other substituents of each pectin vary greatly with the plant source, tissue and developmental stage, reflecting the structure of pectin polysaccharides. of high complexity. [0003] At present, the structure and ...

Claims

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Application Information

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IPC IPC(8): C12P19/14
Inventor 王仲孚黄琳娟
Owner NORTHWEST UNIV
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