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Coumarin and phenylchromone extract from angelica polymorpha maxim and preparation method and use thereof

A technology of coumarins and extracts, which is applied in the direction of pharmaceutical formulations, drug combinations, plant raw materials, etc.

Inactive Publication Date: 2011-09-21
INST OF PHARMACOLOGY & TOXICOLOGY ACAD OF MILITARY MEDICAL SCI P L A
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no related reports on obtaining coumarin and chromone compound with good anti-inflammatory and analgesic activity from Zijinsha in related research at home and abroad

Method used

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  • Coumarin and phenylchromone extract from angelica polymorpha maxim and preparation method and use thereof
  • Coumarin and phenylchromone extract from angelica polymorpha maxim and preparation method and use thereof
  • Coumarin and phenylchromone extract from angelica polymorpha maxim and preparation method and use thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0060] Embodiment 1: the preparation of Zijinsha extract 1

[0061] After taking 500g of Zijinsha medical material and pulverizing it, heat and reflux extraction with 80% ethanol for 2 times, each extraction for 2 hours, the amount of solvent used is 4000ml, the combined extracts are concentrated to dryness under reduced pressure to obtain 96g of ethanol extract. Weigh 10g of the ethanol extract, add 20ml of water to ultrasonically disperse, then add 100ml of cyclohexane to extract 3 times, evaporate the water layer to dryness, add 20% ethanol to dissolve it, and then put it on a HP20 macroporous resin column with a diameter-to-height ratio of 1 : 8, wash away impurities with 20% ethanol of 5 times of BV after loading the sample, then elute with 85% ethanol of 10 times of BV, collect the latter elution part, concentrate under reduced pressure to dryness, obtain the purple golden sand extract. The total content of coumarins and chromones was determined to be 61%; hydrated oxid...

Embodiment 2

[0062] Embodiment 2: the preparation of Zijinsha extract 2

[0063] Take 100g of Zijinsha medicinal material, crush it and pass it through a 20-mesh sieve, then select HA121-50-01 supercritical extraction device, use 200ml of absolute ethanol as entrainer, and extract for 3 hours at an extraction temperature of 45°C and an extraction pressure of 35MPa. The extract was evaporated to dryness under reduced pressure at 40° C. to obtain 5.2 g of supercritical extract. Weigh 5.2g of the crude extract of Zijinsha, add 10ml of water to ultrasonically disperse, then add 50ml of petroleum ether to extract 3 times, evaporate the water layer to dryness, add 30% ethanol to dissolve, and then put it on a HP20 macroporous resin column, the resin column diameter is high Ratio 1:8, wash away impurities with 5 times BV of 30% ethanol after loading the sample, then elute with 8 times BV of 80% ethanol, collect the latter eluted part, concentrate to dryness under reduced pressure, and obtain Zi...

Embodiment 3

[0064] Embodiment 3: the preparation of Zijinsha extract 3

[0065] Take 100 g of Zijinsha medicinal material and pulverize it, heat and reflux with 50% acetone to extract twice, each extraction for 1 hour, the amount of solvent used is 1000 ml, combine the extracts, concentrate to dryness under reduced pressure, and obtain 12.5 g of acetone extract. Weigh 5g of acetone extract, add 10ml of water to ultrasonically disperse, then add 60ml of ethyl acetate to extract for 3 times, concentrate the extract into a paste, add petroleum ether, let stand after ultrasonic for 30 minutes, precipitate out, filter, and use petroleum for precipitation Washed with ether and dried to obtain the extract of Zijinsha. The total content of coumarins and chromones was determined to be 52%; hydrated oxidized precurolactone, selvinol, 3'R-(+)-hexaphenol, 3'-O-acetyl The content (weight %) of holachol and isoimperatorin were 14.3%, 0.9%, 1.1%, 0.8%, 2.8%, respectively.

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Abstract

The invention relates to an angelica polymorpha maxim extract, which contains coumarin and phenylchromone components, wherein the coumarins include one or more of oxypeucedanin hydrate, pabulenol, isoimperatorin, osthol, bergapten, imperatorin, 22,23-dihydroavermectin, isopimpinellin and oxypeucedanin ethylether; and the phenylchromones include one or more of 3'R-(+)hamaudol, 3',-O-acetylhamaudol, angeliticin and ledebouriellol. The invention also relates to a preparation method, a quality control method and a medicinal composition of angelica polymorpha maxim extract and use of the angelica polymorpha maxim extract in the preparation of medicines or products for treating pain and / or inflammatory disorders. The experiments on the pharmacodynamic and acute toxicity of the extract indicate that the extract has the advantages that: the active ingredients are clear; the medicinal effect is remarkable; the toxic and side effects are small; the product quality is high; the dosage is small; the quality is controllable; and the like.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine and chemical medicine, in particular, the invention relates to the extract of Zijinsha rich in coumarin and chromones, its preparation method and its application in medicine for treating pain and / or inflammatory diseases . Background technique [0002] Zijinsha is the root and rhizome of Angelica polymorpha Maxim, a plant belonging to Umbelliferae. The main effects are expelling wind, dispelling cold, drying dampness and relieving pain. It is mostly used for cold and stuffy nose, stomach pain, abdominal pain, chest and hypochondriac pain, rheumatic joint pain, bruises, snake bites and other symptoms. Modern pharmacological research has found that Zijinsha has good curative effects in antibacterial, antispasmodic, anti-ulcer, and immune regulation. [0003] Zijinsha mainly contains coumarins, volatile oil and other components. So far, most of the compounds isolated from Zijinsha by d...

Claims

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Application Information

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IPC IPC(8): A61K36/23A61P29/00A61P19/02A61P19/04A61P19/06A61K125/00
CPCY02P20/54
Inventor 杨郁于能江赵毅民梁菲菲
Owner INST OF PHARMACOLOGY & TOXICOLOGY ACAD OF MILITARY MEDICAL SCI P L A
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