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Bacillus subtilis for simultaneously degrading zearalenone and cellulose and application thereof

A technology of Bacillus subtilis and zearalenone, which is applied in the field of Bacillus subtilis and its cultivation, can solve the problems of endangering the health of animals and humans, and low cellulose degradation, so as to benefit the health of animals, improve utilization rate, and operate simple effect

Active Publication Date: 2011-09-14
河南亿万中元生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The technical problem to be solved by the present invention is to screen out a strain of Bacillus subtilis that has the function of degrading zearalenone and cellulose at the same time, thereby simultaneously solving the problem that zearalenone residues in the feed are harmful to animals and human health, and the feed The problem of low cellulose degradation in

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  • Bacillus subtilis for simultaneously degrading zearalenone and cellulose and application thereof
  • Bacillus subtilis for simultaneously degrading zearalenone and cellulose and application thereof
  • Bacillus subtilis for simultaneously degrading zearalenone and cellulose and application thereof

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Embodiment 1

[0044] The screening method of embodiment 1 bacterial strain

[0045] The strain screening process is as follows: figure 1 As shown, the specific steps are as follows:

[0046] 1) Screening of bacterial strains: In this study, strains were screened from animal intestinal contents, moldy feed, moldy food and natural environment.

[0047] Seed liquid preparation: Mix each intestinal content sample and the sample obtained from the environment with twice the volume of sterile water, place in a sterilized 18mm×180mm test tube, shake overnight; draw 0.1ml of the supernatant, respectively Mix it with 0.8ml of screening medium, and shake it in a 15°C incubator for 12 hours to obtain a seed solution.

[0048] Wherein, the formula of screening medium is: 0.25g NaH 2 PO 4 , 1.0g NH 4 NO 3 , 0.25g MgSO 4 .·7H 2 O, 0.001g FeSO 4 , 17g agar, 2.0g glucose, add distilled water to 1000ml, pH 7.0; steam sterilization at 121°C for 20min.

[0049] Preliminary screening of samples: Add 1...

Embodiment 2

[0061] Embodiment 2 The cultivation method of Bacillus subtilis of the present invention

[0062] Get Bacillus subtilis Bacillus subtilis ANSB01G CGMCC No.42971.5ml (viable bacteria concentration is 10 9 CFU / ml), inoculated in 80ml medium for shake flask fermentation culture, the fermentation temperature is 37°C, the pH value is 7.0, the rotation speed is 200r / min, and the fermentation time is 24h.

[0063] Among them, the shake flask fermentation medium is composed of the following components: tryptone 10g, yeast extract 2g, glucose 2g, beef extract 3g, sodium chloride 4g, disodium hydrogen phosphate 3g, magnesium sulfate heptahydrate 1.0g, distilled water 1000ml , pH value is 7.2.

[0064] After the fermentation, the fermentation broth was stored in a refrigerator at 4°C for later use.

Embodiment 3

[0065] Embodiment 3 The cultivation method of Bacillus subtilis of the present invention

[0066] Get Bacillus subtilis Bacillus subtilis ANSB01G CGMCC No.42970.5ml (viable bacteria concentration is 10 9 CFU / ml), inoculated in 50ml medium for shake flask fermentation culture, the fermentation temperature is 30°C, the pH value is 7.0, the rotation speed is 180r / min, and the fermentation time is 20h.

[0067] Among them, the shake flask fermentation medium is composed of the following components: tryptone 8g, yeast extract 1.5g, glucose 1.5g, beef extract 2g, sodium chloride 3g, disodium hydrogen phosphate 2.5g, magnesium sulfate heptahydrate 0.5g , Distilled water 800ml, pH value is 7.0.

[0068] After the fermentation, the fermentation broth was stored in a refrigerator at 4°C for later use.

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Abstract

The invention relates to a bacillus subtilis ANSB01G CGMCC No. 4297 for simultaneously degrading zearalenone and cellulose and a culturing method thereof. The invention also relates to a method for degrading the zearalenone and the cellulose by using a fermentation liquor of a bacterial strain; by using the method, the degradation rate of the zearalenone can reach 83% while the zearalenone reacts with the bacillus subtilis for 72 hours; and the enzyme activity of the cellulose of the fermentation liquor of the bacillus subtilis is 198.9 U / g. The bacillus subtilis provided by the invention has the advantages of high activity, strong specificity, mild action effect and no damage to nutrition constituents in a feed during degrading the zearalenone; moreover, by using the bacillus subtilis, the cellulose can be also degraded to increase the feed conversion rate.

Description

technical field [0001] The invention relates to bacillus subtilis, in particular to a bacillus subtilis capable of simultaneously degrading zearalenone and cellulose, a cultivation method and application thereof. Background technique [0002] Zearalenone (Zearalenone, ZEN) is a kind of mycotoxin that is widely and seriously polluting grain, food and feed. ZEN is a kind of lactone compound of 2,4-dihydroxybenzoic acid, which has estrogen-like effect and can irreversibly bind to estrogen receptors in the uterus to affect the reproductive physiology of animals. Animals eating ZEN-contaminated feed can cause weight loss or disease, and can also lead to residues of toxins and metabolites in animal products and their processed products. Clinical signs of ZEN intoxication in animals include reduced feed conversion, changes in organ weights, decreased fertility, and behavioral abnormalities. ZEN that enters the human body indirectly through the food chain also seriously threatens ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C07K1/14A23L1/015A23K1/16C12R1/125A23K10/18A23K20/10A23L5/20
Inventor 计成马秋刚雷元培赵丽红高欣
Owner 河南亿万中元生物技术有限公司
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