Nano complex particles with targeted MR (magnetic resonance) development and photothermal therapy performance and preparation method thereof

A nanocomposite and photothermotherapy technology, which is applied to medical preparations containing active ingredients, wave energy or particle radiation treatment materials, preparations for in vivo experiments, etc., can solve the problem of high recurrence rate and difficult therapeutic effect. Evaluation, difficult early diagnosis and discovery of tumors, etc., to achieve clear effects, no chemotherapy side effects, and large demand

Inactive Publication Date: 2011-09-07
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, irregular or diffuse tumors are not only difficult to be diagnosed early, but also difficult to directly evaluate the effect of treatment, and the recurrence rate is very high
There is also no literature reporting a nanocomposite particle with both targeted MR imaging and photothermotherapy properties.

Method used

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  • Nano complex particles with targeted MR (magnetic resonance) development and photothermal therapy performance and preparation method thereof
  • Nano complex particles with targeted MR (magnetic resonance) development and photothermal therapy performance and preparation method thereof

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Embodiment 1

[0038] Decomposition of FeCl in Chitosan Solution by Hydrothermal Synthesis 3 ·6H 2 O prepared from Fe 3 o 4 Single crystal oriented-attached Fe 3 o 4 Crystal clusters (CNCs), because the surface layer of chitosan is rich in amino groups, it has good coordination ability to transition metals. The seed growth method is used to grow gold nanoshells on the surface of CNCs, and then modify the antibody molecules on the surface of the gold shells to finally obtain Nanocomposite particles (CNCs@Au@EGRF) with tumor-targeted MR imaging and photothermotherapy properties. Specific steps are as follows:

[0039] Step (a) Fe 3 o 4 Preparation of colloidal nanocrystal clusters (CNCs):

[0040] Dissolve ferric chloride (1.35g, 5mmol) in 40mL ethylene glycol and stir to obtain a clear solution, then add 3.6g sodium acetate and 1g chitosan (molecular weight 5000), stir for 30min and seal it in a 50mL polytetrafluoroethylene React in a lined reactor at 200°C for 8h. After the reactio...

Embodiment 2

[0048] Step (a): Dissolve ferric chloride (1.35g, 5mmol) in 40mL ethylene glycol and stir to obtain a clear solution, then add 3.6g sodium acetate and 2g chitosan (molecular weight 10000), stir for 30min and seal in a 50mL tape React at 220°C for 8h in a reactor with a Teflon liner. After the reaction solution was cooled to room temperature, it was centrifuged and washed with ethanol to obtain a black product, and CNCs with a diameter of about 90 nm were obtained.

[0049] Step (b): 2.5mL HAuCl 4 (20mM) solution was added to 10mL CNCs (1mg mL), sonicated at room temperature for 30min, then quickly added 3 times excess KBH4 and stirred vigorously for 3min, separated and washed by an external magnetic field to obtain CNCs@Au seeds, and re-diluted to 1mg mL of the solution is ready to use.

[0050] Step (c): CNCs@Au seeds (1mL), HAuCl 4 (20mM, 2mL) solution and Na 2 CO 3 (20mM, 5mL) solution was placed in a 25mL flask, and stirred evenly by magnetic force at high speed. Aft...

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Abstract

The invention relates to a method for preparing targeted metal nano complex particles with a magnetic resonance contrast imaging function and a near infrared photothermal ablation treatment function in the technical field of nanometers. The method comprises the following steps of: preparing Fe3O4 nanocrystal clusters with high magnetic saturation strength and superparamagnetic properties by a hydrothermal method, wherein the surfaces of the Fe3O4 nanocrystal clusters are coated with chitosan, so that the Fe3O4 nanocrystal clusters have extremely high water dispersibility and remodification properties; then loading gold nanoparticles on the Fe3O4 nanocrystal clusters to form a nano gold shell by a seed growing method; and finally fixing an EFRF antibody on the surface of the gold shell by using a chemical bond to obtain a multifunctional targeted nanoparticle complex. The targeted metal nano complex particles have an MR (magnetic resonance) development effect and an photothermal tumor ablation effect, can be widely applied to the fields of early diagnosis of tumors, tumor treatment and the like, can also be used for evaluation before or after treatment, intraoperative detection andthe like and has the advantages of recycling, no chemo-treatment toxicity and the like.

Description

technical field [0001] The invention relates to the technical field of medical detection reagents, in particular to a nanomaterial composite particle and a preparation method thereof. Specifically, chitosan is coated on the surface of superparamagnetic triiron tetroxide nanocrystal clusters, and then the chitosan is used to Amino groups on the surface of the sugar can be used to fix and grow nano-gold shells, which can be applied to magnetic resonance imaging and photothermal ablation of tumors after further connection with tumor-targeting antibodies. Background technique [0002] Cancer is a common and frequently-occurring disease that seriously affects human health. Early diagnosis and efficient treatment of cancer have always been the difficulty and focus of research. Nanomaterials have a good ability to penetrate biological membranes, and nanomaterials with optical and magnetic properties have become one of the most potential means of cancer diagnosis and treatment. [...

Claims

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Application Information

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IPC IPC(8): A61K49/18A61K49/06A61K49/16A61K41/00A61P35/00
Inventor 董生孟小茜孟令杰袁正
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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