Microbiological oxidation and reduction coupling leaching method for valuable metal in manganese oxide ore
A technology for manganese oxide ore and valuable metals, applied in the field of microbial metallurgical beneficiation, can solve the problems of low quantity and slow growth, and achieve the effects of high total biomass, improved efficiency and fast growth
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Embodiment 1
[0021] Embodiment 1: Utilize pyrite as the provider of ferrous sulfate
[0022] 1. Domestication of leaching strains
[0023] (1) Expanded cultivation of ore-leaching bacteria
[0024] Alicyclobacillus BM strain was inoculated into the culture medium and cultured at 25-35°C until OD 600 Stop culturing when reaching 0.8-1.0, obtain the expanded culture medium of BM bacterial strain;
[0025] The medium is BAM medium. In order to increase the carbon source of the medium and adapt the BM strain to a higher concentration of ferrous ion solution, glucose with a concentration of 0.5% to 1% by mass and 4 to 20% by mass of sulfurous acid were added to the BAM medium. Iron; adjust the pH of the medium to 1.5 to 5. Shake flask culture is used for culture, the rotation speed is 100 rpm to 180 rpm, and the culture temperature is 25 to 35°C.
[0026] (2) Domestication of ore-leaching bacteria:
[0027] a. Bacteria adaptation to pyrite in domesticated seed solution
[0028] Based on ...
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