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Method for removing residual disinfectant left after disinfection by capturing with magnetic beads

A technology of disinfectant and magnetic beads, which is applied in the fields of biochemical equipment and methods, and the determination/inspection of microorganisms, can solve problems such as difficulty and complicated screening of neutralizers, and achieve the effect of simple method.

Inactive Publication Date: 2011-08-17
浙江省质量技术监督检测研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the shortcomings of complex and difficult screening of neutralizers in the step of removing residual disinfectants, the present invention provides a method for using magnetic beads to capture and remove residual disinfectants after sterilization

Method used

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  • Method for removing residual disinfectant left after disinfection by capturing with magnetic beads
  • Method for removing residual disinfectant left after disinfection by capturing with magnetic beads
  • Method for removing residual disinfectant left after disinfection by capturing with magnetic beads

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1: This example compares the effects of different amounts of bare magnetic beads 1111# on the adsorption rate of Escherichia coli.

[0013] 1.1 Materials and reagents: including bare magnetic beads 1111#, indicator bacteria and magnetic frame.

[0014] Among them, the bare magnetic beads 1111# are provided by Hangzhou Bioer Technology Co., Ltd. The indicator bacteria was Escherichia coli (ATCC 25922), provided by China Industrial Culture Collection Center. The magnetic frame is produced by British MATRIX company.

[0015] 1.2 Method

[0016] 1.2.1 Bacterial suspension preparation: Escherichia coli was isolated and cultivated, and a single typical colony was taken to inoculate the slant of nutrient agar medium. After cultivation, fresh slant culture cultured for 24 hours was taken, eluted with tryptone saline, and diluted into Bacteria content is 3×10 3 ~3×10 4 cfu / ml bacterial suspension.

[0017] 1.2.2 Determination of the number of bacteria captured by ...

Embodiment 2

[0023] Embodiment 2: In this embodiment, the effects of the resting time before being put into the magnetic frame on the adsorption rate of Escherichia coli are compared.

[0024] 2.1 Materials and reagents

[0025] Bare magnetic beads 1111#: Provided by Hangzhou Bioer Technology Co., Ltd.

[0026] Indicator bacteria: Escherichia coli (ATCC 25922), provided by China Industrial Culture Collection Center.

[0027] Magnetic frame: produced by British MATRIX company.

[0028] 2.2 Method

[0029] Determination of the number of bacteria captured by magnetic beads: Add 20 ul bare magnetic beads 1111# and 0.5 ml of the bacterial suspension prepared in 1.2.1 to a 2 ml sterile test tube, shake gently, let stand for 5 min, 10 min, 15 min, Shake gently while taking out intermittently. Take out the test tube and put it on the magnetic stand, let it stand for adsorption for 10 min, blot the liquid and place it on the blank plate A, and count it as the number of residual bacteria. Immed...

Embodiment 3

[0035] Embodiment 3: In this embodiment, the influence of different resting times on the magnetic frame on the adsorption rate of Escherichia coli is compared.

[0036] 3.1 Materials and reagents

[0037] Bare magnetic beads 1111#: Provided by Hangzhou Bioer Technology Co., Ltd.

[0038] Indicator bacteria: Escherichia coli (ATCC 25922), provided by China Industrial Culture Collection Center.

[0039] Magnetic frame: produced by British MATRIX company.

[0040] 3.2 Method

[0041] Determination of the number of bacteria captured by magnetic beads: add 20 ul bare magnetic beads 1111# to a 2 ml sterile test tube, and 0.5 ml of the bacterial suspension prepared in 1.2.1, shake gently, let stand for 10 minutes, and take out gently during the period shake. Take out the test tube and put it on the magnetic stand, let it stand for adsorption for 5min, 10min, and 15min, blot the liquid and place it on the blank plate A, and count as the number of residual bacteria. Immediately ad...

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Abstract

The invention discloses a method for removing residual disinfectant left after disinfection by capturing with magnetic beads. The method comprises the following steps of: adding residual-disinfectant-containing disinfected liquid and naked magnetic beads into a sterile plastic pipe, shaking lightly and standing for 5-15 minutes; putting the sterile plastic pipe onto a magnetic frame, standing and adsorbing for 5-10 minutes and fully absorbing liquid in the sterile plastic pipe to dryness; adding 0.5ml of disinfected physiological saline into the sterile plastic pipe, uniformly mixing the disinfected physiological saline with naked magnetic beads 1111-model and sucking the mixed liquor into a flat plate; and adding 0.5ml of disinfected physiological saline for cleaning residual bacterial liquid, uniformly mixing and sucking the mixed liquor into the flat plate. Microorganism adsorption is performed on residual-disinfectant-containing disinfected treatment liquid, and the adsorption rate is over 99 percent; by adopting the method, the residual disinfectant can be effectively removed, and screening and concentration adjustment of a neutralizing agent component used in disinfection are avoided; and the method is simpler and more effective. The method disclosed by the invention is suitable for quantitative separation detection of microorganisms, and can be applied to detection, verification and evaluation of the disinfection performance of a disinfectant (bactericide).

Description

technical field [0001] The invention relates to a method for capturing and removing residual disinfectants after sterilization by using magnetic beads, which is mainly used in the detection, identification and evaluation of the sterilization performance of disinfectants (bactericides). Background technique [0002] In order to accurately detect, identify and evaluate the bactericidal performance of disinfectants (bactericides), after a certain sterilization time, a step of removing residual disinfectants is required. At present, the most commonly used method for removing residual disinfectants is the neutralizer method. Commonly used neutralizers are as follows: 5g / L sodium thiosulfate for chlorine-based disinfectants; 3g / L lecithin + 20g / L Tween 80; 10g / L sodium thiosulfate + 20g / L Tween 80 , against oxidizing disinfectants. For each test, the method of removing residual disinfectant needs to be qualified before it can be applied. (Citing "Technical Specifications for Di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06
Inventor 金燕飞厉永刚姜侃
Owner 浙江省质量技术监督检测研究院
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