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Method for increasing sugar yield of cellulase enzymolysis through pretreatment by using ion liquid

An ionic liquid and cellulase technology, applied in the field of cellulose application, can solve problems such as affecting process cost, affecting enzymatic hydrolysis efficiency and sugar yield, etc., and achieves the advantages of improving accessibility, increasing sugar yield and simplifying process. Effect

Inactive Publication Date: 2011-08-17
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Other enzymatic hydrolysis conditions, such as enzyme loading and solid-liquid ratio, will also affect the efficiency of enzymatic hydrolysis and sugar yield, thereby affecting the process cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Sugar yield of rice straw cellulose pretreated with ionic liquid [AMIM]Cl.

[0025] (1) Pretreatment of rice straw cellulose with ionic liquid [AMIM]Cl

[0026] a) Drying the rice straw cellulose at 100° C. for 24 hours, and controlling its particle size to 80 μm to 120 μm.

[0027] b) Accurately weigh 5 g of rice straw and 45 g of ionic liquid [AMIM]Cl, put them together into a 100ml round-bottomed flask, seal it at 130 °C, and stir vigorously for 2 hours until the cellulose is completely dissolved, and the solution turns dark brown at this time Viscous.

[0028] c) Stop stirring, and repeatedly rinse the obtained cellulose ionic liquid solution with deionized water 5 times its volume three times, and finally obtain a white sediment, that is, regenerated cellulose, filter and freeze-dry the product for 48 h and grind it into powder Standby, and use vacuum rotary evaporation to recover the ionic liquid.

[0029] (2) Enzymatic hydrolysis of regenerated cellulose with ...

Embodiment 2

[0035] Sugar Yield of Rice Straw Cellulose Pretreated by Ionic Liquid [BMPy]Br

[0036] (1) Pretreatment of rice straw cellulose with ionic liquid [BMPy]Br

[0037] a) Drying the rice straw cellulose at 100° C. for 24 hours, and controlling its particle size to 80 μm to 120 μm.

[0038] b) Accurately weigh 5 g of rice straw and 45 g of ionic liquid [BMPy]Br, put them together into a 100ml round-bottomed flask, seal it at 130 °C, stir vigorously for 2 hours until the cellulose is completely dissolved, and the solution turns dark brown at this time Viscous.

[0039] c) Stop stirring, and repeatedly rinse the obtained cellulose ionic liquid solution with deionized water 5 times its volume three times, and finally obtain a white sediment, that is, regenerated cellulose, filter and freeze-dry the product for 48 h and grind it into powder Standby, and use vacuum rotary evaporation to recover the ionic liquid.

[0040] (2) Enzymatic hydrolysis of regenerated cellulose with Reesei'...

Embodiment 3

[0054] Sugar yield of corn stover cellulose pretreated by ionic liquid [AMIM]Cl

[0055] (1) Pretreatment of corn stover cellulose with ionic liquid [AMIM]Cl

[0056] a) Drying the corn stalk cellulose at 100° C. for 24 hours, and controlling its particle size to 80 μm to 120 μm.

[0057] b) Accurately weigh 5 g of corn stalks and 45 g of ionic liquid [AMIM]Cl, put them together into a 100ml round-bottomed flask, seal it at 130 °C, stir vigorously for 2 hours until the cellulose is completely dissolved, and the solution turns dark brown at this time Viscous.

[0058] c) Stop stirring, and repeatedly rinse the obtained cellulose ionic liquid solution with deionized water 5 times its volume three times, and finally obtain a white sediment, that is, regenerated cellulose, filter and freeze-dry the product for 48 h and grind it into powder Standby, and use vacuum rotary evaporation to recover the ionic liquid.

[0059] (2) Enzymatic hydrolysis of regenerated cellulose with Rees...

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Abstract

The invention relates to a method for increasing the sugar yield of cellulase enzymolysis through pretreatment by using ion liquid. In the method, natural lignin from different sources is pretreated by using different types of ion liquids to increase the sugar yield of enzymolysis. The method comprises the following steps of: firstly, pretreating natural lignin at certain temperature under fully stirring condition by using an ion liquid; secondly, extracting regenerated cellulose from a mixed liquor and drying; and lastly, adding cellulase which can decompose the regenerated cellulose serving as a substrate into reducing sugar at certain temperature, pH and concentration to generate liquid glucose mainly containing glucose. By adopting the method, the sugar yield of lignin can be remarkably increased, the accessibility and the reaction efficiency of cellulase can be effectively increased, the liquid glucose reaches the fermentation level at the final reaction stage, and the entire technical process is simplified.

Description

technical field [0001] The invention belongs to the field of cellulose application, and in particular relates to a method for improving the sugar production rate of cellulose enzymatic hydrolysis by utilizing ionic liquid pretreatment. Background technique [0002] With the rapid depletion of non-renewable energy resources such as oil, natural gas, coal mines, and metal deposits, the contradiction between the supply and demand of energy and resources has become increasingly prominent, and the development and utilization of new materials have attracted the attention of the world. There are abundant natural resources available in nature, cellulose being an example. Cellulose is the most abundant renewable resource in nature and can be widely used in industrial fields such as papermaking and coatings. The conversion of natural lignocellulose into biochemical products such as ethanol and lactic acid with high economic and social value by means of biotechnology is currently a ho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14
Inventor 袁华张艳娟任杰
Owner TONGJI UNIV
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