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Early identifying method of pistachio male trees and pistachio female trees

A pistachio and ayue technology, applied in the direction of using chemical indicators for analysis, etc., can solve the problems of professional not using ordinary personnel to master and learn, technical difficulty, and difficult to promote, etc., to achieve easy popularization, easy operation, and simple method. Effect

Inactive Publication Date: 2011-08-10
李洁
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method is too professional and does not use ordinary personnel to master and learn, so it is not easy to promote
In addition, chromosome compression technology and karyotype analysis can directly and clearly understand the sex difference between male and female plants, but the technology is difficult and difficult to promote

Method used

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Embodiment Construction

[0009] The method of the present invention is (1) take clean, some fresh leaves without damage by diseases and insect pests, weigh 0.5g of clean male and female plant leaves with main veins removed respectively, adopt the blade cutting method, cut the leaves into shredded tobacco, and place them in In a clean 50ml Erlenmeyer flask, add 0.1%, or 0.2%, or 0.3% concentration of cresol red solution, keep it warm in a 28°C incubator for 72 hours, observe the color change, and use distilled water as a contrast; (2) after treatment The yellow-red leaves are the female plant leaves, and the blue-green leaves are the male plant leaves after treatment, so that the female and male of the corresponding pistachio tree species can be judged.

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Abstract

The invention relates to a chemical detecting method, in particular to an early identifying method of male pistachios and female pistachios. The early identifying method comprises the steps of: (1) collecting clean and non-insect-disease fresh leaves, respectively weighing 0.5 gram of clean male plant leaves without main veins and 0.5 gram of clean female plant leaves without main veins, shearing the leaves into the shape of pipe tobacco by adopting the leaf shearing method, putting the shorn leaves into a clean 50-milliliter triangular flask, adding a cresol red solution with the concentration of 0.1, 0.2 or 0.3 percent, putting the triangular flask into a constant-temperature box at the temperature of 28 DEG C, preserving the temperature for 12 to 72 hours, observing the change of color, and comparing with distilled water; and (2) judging the yellow red leaves after treatment as female plant leaves and the glaucous leaves as the male plant leaves so as to judge the female pistachio seeds and the male pistachio seeds. The early identifying method of male pistachios and female pistachios is rapid and effective, and is easy to operate and master.

Description

technical field [0001] The invention relates to a chemical detection method, in particular to an early identification method for male and female pistachio trees. Background technique [0002] Pistachio is native to the Middle East in West Asia and belongs to the Pistacia genus of the Anacardaceae family. Pistachio is a dioecious plant, and its sex is difficult to distinguish through characteristics such as tree body and branch and leaf shape before fruiting. Early gender identification is necessary for scientific research and production of pistachio new varieties, breeding, planting and gardening. At present, RAPD is used to amplify the genomic DNA of male and female pistachio plants to find out the nucleotide fragments that differ between sexes, that is, to find some genetic markers related to the sex of pistachio, so as to quickly and effectively There are significant differences in the identification of male and female plants, which lays a theoretical foundation for the...

Claims

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Application Information

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IPC IPC(8): G01N31/22
Inventor 李洁
Owner 李洁
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