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Three-step method for producing ''dragon head phoenix tail'' high-quality officinal dendrobium stem material

A technology of Dendrobium candidum, three-step method, which is applied in the field of "three-step method" to produce high-quality Dendrobium candidum material of "dragon head and phoenix tail". The seedling time is short, easy to master, and the effect of simple steps

Active Publication Date: 2012-07-18
DONGGUAN CITY RUISHEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is slow in raising seedlings, and the operation of obtaining provenance is cumbersome and poor in practicability
Therefore, this method, especially the artificial pollination technology in vitro, is difficult to achieve commercial operation.
[0006] Although there are many studies on the cultivation of Dendrobium candidum test tube seedlings, they are limited to the research on tissue culture seedling cultivation, cultivation and improving the survival rate of transplanting, focusing on yield and cultivation methods and techniques, and research on the production quality or quality of Dendrobium candidum not involved yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) Embryo culture directly into seedlings: the mature fruit pods of Dendrobium candidum (Dendrobium candidum seedlings are purchased from the Orchid Association of Yueqing City, Zhejiang Province, and are mature fruit pods obtained by cultivating for 5 months after artificial cross-pollination) through volume percentage 75% alcohol Disinfect the surface for 7 minutes, burn the soaked fruit pods with an alcohol lamp flame for 2-3 seconds, cut open the fruit pods with a scalpel, collect the seed embryos in a sterile serum bottle, add 5ml of sterile water to dilute and suspend, Take 1ml of the suspension, spread it evenly on the surface of sterile LCY medium, cultivate it under dark light for 10 days, and after 10 days, the seed embryos will expand, and then give 1000Lux light during the day (12 hours). After 30 days, the seed embryos germinate into small green buds, transplant the green buds to the LVB medium, transfer the rooted seedlings to the LPY medium after 60 days,...

Embodiment 2

[0046] (1) Embryo culture directly into seedlings: the mature fruit pods of Dendrobium officinale were sterilized with 75% alcohol by volume for 7 minutes, and the soaked fruit pods were burned with an alcohol lamp flame for 2 to 3 seconds, and the scalpel blades were cut open. Collect the seed embryos in a sterile serum bottle, add 5ml of sterile water to dilute and suspend, draw 1ml of the suspension, spread it evenly on the surface of sterile LCY medium, and cultivate it under dark light for 20 days. During the day (12 hours) 1500Lux light. After 45 days, the seed embryos germinate into small green buds, transplant the green buds to the LVB medium, transfer the rooted seedlings to the LPY medium after 70 days, and after 80 days, each cluster of seedlings grows an average of 6 root systems, with an average seedling height of 10cm , move the test-tube seedlings into the greenhouse for hardening for 10 days, control the light at 2000-3000Lux, and the temperature at 23-25°C.

...

Embodiment 3

[0055] (1) Embryo culture directly into seedlings: the mature fruit pods of Dendrobium officinale were sterilized with 75% alcohol by volume for 7 minutes, and the soaked fruit pods were burned with an alcohol lamp flame for 2 to 3 seconds, and the scalpel blades were cut open. Collect the seed embryos in a sterile serum bottle, add 5ml of sterile water to dilute and suspend, draw 1ml of the suspension, spread it evenly on the surface of sterile LCY medium, and cultivate it under dark light for 15 days. 1500Lux light. After 40 days, the seed embryos germinated into small green buds, and the green buds were transplanted to the LVB medium. After 65 days, the rooted seedlings were transferred to the LPY medium. After 70 days, each cluster of seedlings grew an average of 5.5 roots, and the average height of the seedlings was 9.6. cm, used as transplanted seedlings in the greenhouse.

[0056] The LCY medium is adding coconut water and yeast extract to LV-3 medium, wherein every li...

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Abstract

The invention discloses a three-step method for producing a 'dragon head phoenix tail' high-quality officinal dendrobium stem material. The method comprises three steps of embryo culture for direct seedling formation, plug plantation of seedlings in a greenhouse, and culture of a semi-mature seedling epiphytic trunk; and an officinal dendrobium stem production technology is simplified, seedling breeding time is shortened, and the quality and transplanting survival rate of test tube seedlings of officinal dendrobium stem are improved. In the method, the production time of the test tube seedlings is 6 to 7 months, but the conventional test tube seedling breeding time is 10 to 12 months. An officinal dendrobium stem medicinal material prepared by the method has a thick and short stem, the content of polysaccharide from Dendrobium officinale is 36 to 48 percent, and the officinal dendrobium stem medicinal material has a 'dragon head phoenix tail' characteristic in external shape and is a necessary raw material for processing 'dragon head phoenix tail' Tiepi Fengdou. A semi-mature seedling epiphytic trunk technology in the method is suitable for farmers in mountainous areas to cultivate the officinal dendrobium stem, and is low in investment and high in yield; the farmers do not need to build greenhouses and only need to purchase plug seedlings; and the cultivation technology is easy to grasp and is a practical technology for servicing 'Three Rural Issues'.

Description

technical field [0001] The invention belongs to the technical field of plant group cultivation and seedling cultivation and cultivation, and particularly relates to a "three-step method" method for producing high-quality dendrobium officinale materials of "dragon head and phoenix tail". Background technique [0002] Dendrobium candidum is a kind of aerial plant of Orchidaceae. It is a very rare and precious wild Chinese medicinal material. Dendrobium candidum is processed into glumes, also known as Tiepi Fengdou. "Dragon head and phoenix tail" is a treasure of Tiepi Fengdou. Dendrobium officinale is slightly cold in nature, sweet in taste, enters the lung, stomach, and kidney meridian, and has the effects of nourishing yin and tonifying kidney, clearing heat and promoting body fluid, improving intelligence and calming nerves, and strengthening essence and bones. More than a thousand years ago, the Taoist medical classic "Tao Zang" listed Dendrobium officinale as the first o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 廖俊杰李进进李桂锋张建军廖建友
Owner DONGGUAN CITY RUISHEN BIOTECH
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