Preparation method of quantitative microorganism freeze-dried product

Abstract

The invention discloses a preparation method of a quantitative microorganism freeze-dried product. The preparation method comprises the following steps of: diluting microorganisms at a stable period to obtain uniform diluent and preparing the diluent into bacterial suspension with quantitative concentration; mixing the bacterial suspension with a protecting agent to obtain mixed liquor; and bottling quantitative mixed liquor, pre-freezing at the temperature of -30 DEG C, freezing and drying in vacuum, and sealing in vacuum to obtain the quantitative microorganism freeze-dried product, whereinthe protecting agent comprises the following components in parts by weight: 0.1-10 parts of water-soluble sugar, 0.1-5 parts of defatted milk powder, 0.1-5 parts of antioxidant, 0.1-20 parts of gelatin and 0-10 parts of active carbon. The microorganism freeze-dried product prepared by adopting the preparation method contains basically quantitative microorganisms, and is convenient to use; and theactivity of the microorganisms can be ensured when the product is stored at the temperature of below 8 DEG C, and the biological and chemical properties of the microorganisms do not change basically within the retention period.

Description

Technical field [0001] The present invention involves a method of preparing a microbial frozen -drying product, which specializes in a preparation method for quantitative microbial frozen dried products. Background technique [0002] A variety of bacteria is an important biological resource. The storage of bacteria is an important microbial basic work. Internationally, it has always paid more attention to the preservation and sharing exchanges of microbial fungus resources.As early as the end of the 19th century, Europe began to have some standardized bacteria storage agencies; in the early 20th century, some famous bacteria storage agencies were established one after another. The NRRL in 1904 was established in the United States. In 1925Become the world's largest bacterial storage supply center. [0003] The preservation of the mass control strain has been studied abroad. The principle is the same as the preservation of general bacteria. The current preservation methods adopted ...

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Problems solved by technology

[0007] At present, domestic grass-roots units and most production enterprises generally do not have strain management specialists, the preservation facilities are not complete, and the professional operation level of microbiological inspection personnel is not high, so it is relatively easy to cause death, Variation, contamination, etc.

Moreover, the preserved strains often need to go through cumbersome processes such as resuscitation, transfer, and dilution during use, which not only consumes time and a lot of manpower and material resources, but also may affect the accuracy and reliability of the test results, seriously reducing work. Efficiency, so that the quality of the product cannot be guaranteed. If the experiment fails due to the strain problem, re-sampling is required, which delays the sales of the product and increases the cost of the enterprise.

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