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Composition and use thereof

A composition and peroxidase technology, which is applied to biochemical equipment and methods, and the determination/inspection of microorganisms, can solve the problems of insignificant color change gradient and inaccurate test results in test results, and achieve stable color development results. , the effect of expanding the detection concentration range

Active Publication Date: 2013-12-04
SHANGHAI ADICON CLINICAL LAB LNC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the content of the detected substance is high, for example, when the concentration of glucose in the sample is greater than 3g / L, the color gradient of the detection results of samples with different glucose concentrations is not obvious, making the detection results inaccurate

Method used

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  • Composition and use thereof
  • Composition and use thereof
  • Composition and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 The composition without chromogenic agent stabilizer II is used to detect the content of glucose in urine

[0038]Immerse Whatman 3MM test paper in solution I, bake in a 70°C oven for 25 minutes, then immerse in solution II, and bake in a 70°C oven for 15 minutes.

[0039] Solution I

[0040] medicine

Dosage per 1000ml solution

purified water

1000ml

citrate buffer saline

1mol, pH5.5

glucose oxidase

120ku

Horseradish peroxidase

50ku

[0041] Solution II

[0042] medicine

Dosage per 1000ml solution

acetone

1000ml

3,3',5,5'-Tetramethylbenzidine

3g

1-Phenylsemicarbazide

0.4-1.2g

[0043] Cut the above test paper into 5mm×5mm squares and paste it on a plastic card, immerse it in the standard solution with glucose content of 0.5g / L, 1.0g / L, 3.0g / L, and 10g / L respectively, take out the test paper and absorb it. Remove the excess solution, ...

Embodiment 2

[0047] Example 2 The composition containing 4-phenylsemicarbazide is used to detect the content of glucose in urine

[0048] Immerse Whatman 3MM test paper in solution I, bake in a 70°C oven for 25 minutes, then immerse in solution II, take it out and bake in a 70°C oven for 15 minutes.

[0049] Solution I

[0050] medicine

Dosage per 1000ml solution

purified water

1000ml

citrate buffer saline

1mol, pH5.5

glucose oxidase

120ku

Horseradish peroxidase

50ku

[0051] Solution II

[0052] medicine

Dosage per 1000ml solution

Absolute ethanol

1000ml

3,3',5,5'-Tetramethylbenzidine

3g

1-Phenylsemicarbazide

0.4g

4-Phenylsemicarbazide

0.1～2g

[0053] Cut the above test paper into 5mm×5mm squares and paste it on a plastic card, immerse it in the standard products with glucose content of 0.5g / L, 1.0g / L, 3.0g / L, and 10g / L respectively, take out the te...

Embodiment 3

[0057] Example 3 The composition containing bisphenol A or gallic acid detects the content of glucose in urine

[0058] Immerse Whatman 3MM test paper in solution I, bake in a 70°C oven for 25 minutes, then immerse in solution II, take it out and bake in a 70°C oven for 15 minutes.

[0059] Solution I

[0060] medicine

Dosage per 1000ml solution

purified water

1000ml

citrate buffer saline

1mol, pH5.5

glucose oxidase

120ku

Horseradish peroxidase

50ku

[0061] Solution II

[0062] medicine

Dosage per 1000ml solution

Absolute ethanol

1000ml

3,3',5,5'-Tetramethylbenzidine

3g

1-Phenylsemicarbazide

0.4g

Bisphenol A or gallic acid

0.8g

[0063] Cut the above test paper into 5mm×5mm squares and paste it on a plastic card, immerse it in the standard products with glucose content of 0.5g / L, 1.0g / L, 3.0g / L, and 10g / L respectively, take out the test...

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Abstract

The invention provides a composition for measuring peroxide content in solution or the content of a peroxide generated by reaction. The composition comprises a peroxidase, a buffer salt, a color-developing agent and two color stabilizers, wherein one color stabilizer has a higher reducing capacity than the color-developing agent, and the other color stabilizer has a reducing capacity which is between that of the color-developing agent and that of the first color stabilizer. The composition enlarges the test range of the tested materials and makes color-developing effect stable.

Description

technical field [0001] The invention relates to a composition and its application, in particular to a composition for measuring the content of peroxide and its application for measuring the content of hydrogen peroxide. Background technique [0002] The dry chemical test paper detection method can obtain various chemical or biological information of the tested sample in a short time with a small amount of sample. As a screening test, dry chemical detection method is widely used in the judgment of medical inspection and food safety inspection. For example, use dry chemical test strips to detect glucose and red blood cells in urine samples, detect alanine aminotransferase in blood, and detect pesticide residues in vegetables. [0003] The dry chemical test paper detection method includes two detection steps of "dipping" and "reading". According to different detection items, the reaction reagents are pre-dried and fixed on the membrane carrier of the dry chemical test paper. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/28
Inventor 李和平商涛廖青青
Owner SHANGHAI ADICON CLINICAL LAB LNC
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