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Reagent and method for stabilizing alkaline phosphatase or marker of alkaline phosphatase

A stabilizer and phosphatase technology, applied in biochemical equipment and methods, enzyme stabilization, biological testing, etc., can solve problems such as stable preservation of alkaline phosphatase not involved, and achieve improved stability, extended shelf life, and improved product quality. quality effect

Active Publication Date: 2011-07-06
SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Likewise, the patent does not address the stable preservation of alkaline phosphatase in solution

Method used

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  • Reagent and method for stabilizing alkaline phosphatase or marker of alkaline phosphatase
  • Reagent and method for stabilizing alkaline phosphatase or marker of alkaline phosphatase
  • Reagent and method for stabilizing alkaline phosphatase or marker of alkaline phosphatase

Examples

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Comparison scheme
Effect test

Embodiment 1

[0064] Alkaline phosphatase was added to the stabilizer solution described below to prepare an enzyme solution with a final concentration of 5ug / mL, and placed in a constant temperature and humidity chamber at 37°C for accelerated testing. Taking the enzyme solution as a sample, the remaining activity ratio of alkaline phosphatase in the solution (the ratio of the activity after the accelerated test to the activity after preparation) was determined by using the Mindray automatic biochemical analyzer BS300 and the matching alkaline phosphatase assay kit.

[0065] Prepare the solution according to the following formula:

[0066] BSA 1% (g / 100ml)

[0067] MgCl 2 1mM

[0068] Proclin 300 0.05% (g / 100ml)

[0069] Tris 50mM

[0070] Adjust pH with hydrochloric acid to pH 7.5

[0071] As can be seen from the results in Table 1, in the stabilizer of this example, the alkaline phosphatase was accelerated, and after one week, 26% of the enzyme activity remained, and n...

Embodiment 2

[0075] Alkaline phosphatase was added to the stabilizer solution described below to prepare an enzyme solution with a final concentration of 5ug / mL, and placed in a constant temperature and humidity chamber at 37°C for accelerated testing. Taking the enzyme solution as a sample, the remaining activity ratio of alkaline phosphatase in the solution (the ratio of the activity after the accelerated test to the activity after preparation) was determined by using the Mindray automatic biochemical analyzer BS300 and the matching alkaline phosphatase assay kit.

[0076] Prepare the solution according to the following formula:

[0077] BSA 1% (g / 100ml)

[0078] ZnCl 2 , 0.1mM

[0079] NaCl 8g / L

[0080] MgCl 2 1mM

[0081] Proclin 300 0.05% (g / 100ml)

[0082] Tris 50mM

[0083] Adjust pH with hydrochloric acid to pH 7.5

[0084] As can be seen from the results in Table 2, in the stabilizer of this example, the alkaline phosphatase was accelerated, and the enzyme acti...

Embodiment 3

[0088] Alkaline phosphatase was added to the stabilizer solution described below to prepare an enzyme solution with a final concentration of 5ug / mL, and placed in a constant temperature and humidity chamber at 37°C for accelerated testing. Taking the enzyme solution as a sample, the remaining activity ratio of alkaline phosphatase in the solution (the ratio of the activity after the accelerated test to the activity after preparation) was determined by using the Mindray automatic biochemical analyzer BS300 and the matching alkaline phosphatase assay kit.

[0089] Prepare the solution according to the following formula:

[0090] BSA 1% (g / 100ml)

[0091] ZnCl 2 , 0.1mM

[0092] NaCl 100g / L

[0093] Casein 1% (g / 100ml)

[0094] Gelatin 1% (g / 100ml)

[0095] MgCl 2 1mM

[0096] Proclin 300 0.05% (g / 100ml)

[0097] Tris 50mM

[0098] Adjust pH with hydrochloric acid to pH 7.5

[0099] As can be seen from the results in Table 3, in the stabilizer of this example...

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Abstract

The invention discloses and relates to a stabilizing agent of alkaline phosphatase or a marker of the alkaline phosphatase, a preparation method of the stabilizing agent and a method for stabilizing the alkaline phosphatase or the marker of the alkaline phosphatase by using the stabilizing agent. The invention also relates to the alkaline phosphatase or a marker reagent of the alkaline phosphatase and a preparation method thereof. The invention also relates to a kit which comprises the stabilizing agent and the alkaline phosphatase or the marker of the alkaline phosphatase disclosed by the invention. The stabilizing agent disclosed by the invention can stably preserve the alkaline phosphatase or the marker of the alkaline phosphatase for a long time, so that the retention period of the alkaline phosphatase or a marker solution of the alkaline phosphatase is prolonged.

Description

technical field [0001] The present invention relates to enzyme stabilizers. Specifically, the present invention relates to a stabilizer for alkaline phosphatase or a marker thereof, and a method for stabilizing alkaline phosphatase or a marker thereof using the stabilizer comprises the stabilizer and alkaline phosphatase or a marker thereof reagents. Background technique [0002] Alkaline phosphatase (alkaline phosphatase, AP) is an enzyme that widely exists in animals, plants, microorganisms, etc. Alkaline phosphatase, which is frequently used, is extracted from calf intestinal mucosa and Escherichia coli and consists of multiple isozymes. In an appropriate buffer, alkaline phosphatase can catalyze the hydrolysis of nitrophenyl phosphate (PNP), sodium β-glycerophosphate, naphthyl phosphate, 3-(2-spiroadamantane)-4-methoxy-4 Chromogenic and chemiluminescent substrates containing phosphate groups such as (3-phosphoryloxy)-phenyl-1,2-dioxetane (AMPPD). In view of this, alk...

Claims

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Application Information

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IPC IPC(8): C12N9/96G01N33/53
CPCG01N33/535C12N9/16C12Y301/03001C12N9/96
Inventor 钱纯亘张裕平
Owner SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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