Pretreatment method and special reagent for living body transportation of parents of cephalopods
A technology for live transportation and cephalopods, which is applied in the field of pretreatment methods and special reagents for the transportation of cephalopods in vivo to achieve stable performance
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Embodiment 1
[0020] Embodiment 1: refer to Figure 1 to Figure 2 , the pre-treatment method of cephalopod parent living transportation, it comprises the following steps:
[0021] ① Dissolution at room temperature: Dissolve the anesthetic evenly in a container filled with water, so that the concentration of the anesthetic is 5ppm to 12ppm; 5ppm is preferred in this embodiment.
[0022] ② Gradient cooling: After the treatment in step ①, put the cephalopod parent body into the container, and cool down to 7°C to 18°C through a gradient; in this embodiment, the temperature is preferably lowered to 7°C.
[0023] ③Transportation preparation: after step ②, add NaCl and CaCl to the container 2 , so that the concentration of NaCl in the water increases from 0.5g / L to 3g / L and CaCl 2 The concentration of the drug increased from 50mg / L to 500mg / L. In this embodiment, it is preferable to control the concentration of NaCl in the water body to rise by 0.5g / L, and the concentration of CaCl 2 Concent...
Embodiment 2
[0033] Embodiment 2: refer to Figure 1 to Figure 2 , the pre-treatment method of cephalopod parent living transportation, it comprises the following steps:
[0034] ① Dissolution at room temperature: Dissolve the anesthetic evenly in a container filled with water, so that the concentration of the anesthetic is 12ppm;
[0035] ② Gradient cooling: After the treatment in step ①, put the cephalopod parents into the container, and cool down by 13°C through a gradient;
[0036] ③Transportation preparation: after step ②, add NaCl and CaCl to the container 2 , so that the concentration of NaCl in the water body increases by 2g / L and CaCl 2 The concentration rises by 300mg / L.
[0037] The anesthetic was propyl aniline acetaminobenzoate hydrochloride.
[0038] In step ③, zeolite particles equivalent to twice the weight of NaCl are also added.
[0039] The temperature gradient used for gradient cooling is 0.5°C, and the interval between each gradient is 60 minutes.
[0040] The pr...
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