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Production process of microbial preparation for baking tobacco leaves

A microbial preparation and production process technology, applied in the field of microbial preparation production technology, can solve the problem of huge energy consumption of equipment, and achieve the effects of saving investment and energy consumption, strong resistance to harsh environments, and simple and easy process

Inactive Publication Date: 2011-06-29
KUNMING GUIXUN TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The post-treatment process inevitably brings expensive equipment investment, huge energy consumption and pollution sources formed by fermentation broth.

Method used

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  • Production process of microbial preparation for baking tobacco leaves
  • Production process of microbial preparation for baking tobacco leaves
  • Production process of microbial preparation for baking tobacco leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Fermentation process parameters of bacterial strain YN91 and bacterial strains YN114, YN113, YN104 of the present invention. The fermentation medium of strain YN91 is: wheat bran 1.5%, urea 0.5%, NaCl 0.5%. Fermentation process parameters: temperature 50°C, time 18h, stirring speed 125rpm. The enzyme activity level is around 6960U / mL. The fermentation medium of strain YN114 is: soluble starch 0.5%, beef extract 1.0%, CaCO 3 0.3%. Fermentation process parameters: temperature 43°C, time 36h, stirring speed 125rpm. The enzyme activity level is around 28(U / mL). The fermentation parameters of strains YN113 and YN104 were the same as those of YN114.

[0025] The specific method is as follows: culture medium preparation, take 5-15mL of the waste tobacco leaf extraction stock solution soaked for 2-3 days in a 500mL triangular flask, add 95-85mL of tap water, add 2.5g of agar powder, the pH is natural, after shaking well, use 15 lb. Autoclave for 30 minutes. Af...

Embodiment 2

[0033] Example 2: In a 50L Bio-automatic fermenter, use the "Method for High Temperature Separation of Microorganisms on the Surface of Tobacco Leaves with Pure Tobacco Leach Liquid Solid Plate", then 150mL of the protease-producing high-temperature bacterial strain YN114 seed culture solution isolated in step A was inoculated into Put 2.4Kg of crushed waste tobacco leaves and 30L of tap water into the sterilized medium, rotate at 120rpm, cultivate at 45±5°C, add 30g of manganese sulfate that was filtered and sterilized after 46 hours of fermentation, stir for 2 hours, put The cans are divided into plastic bottles and sealed for later use. The product is stored for half a year, and the number of viable bacteria is greater than 500×10 8 cfu / mL, protease activity was 28.30U / mL. Others are the same as the examples.

Embodiment 3

[0034] Example 3: In a 50L Bio-automatic fermenter, use the "Method for High Temperature Separation of Microorganisms on the Surface of Tobacco Leaves from Pure Tobacco Leach Liquid Solid Plate", then 150mL of the protease-producing high-temperature bacterial strain YN113 liquid isolated in step A is inoculated into 2.4 Kg crushed discarded tobacco leaves and 30L tap water sterilized culture medium, rotating speed 120rpm, culture temperature 50±5°C, when fermented to 40 hours, add filter-sterilized manganese sulfate 30g, stir for 2 hours, put into tank and divide Put it into a plastic bottle and seal it for later use. The storage period of the product is one year, and the number of viable bacteria is greater than 500×10 8 cfu / mL, protease activity was 24.10U / mL. It can still be metabolized during the curing process, improving the quality of cured tobacco leaves such as image 3 shown. The present invention adds 0.1%-0.5% MnSO to the fermentation liquid before the end of fer...

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Abstract

The invention provides a production process of a microbial preparation, which is used for baking tobacco leaves and aims at improving the quality of availability of the tobacco leaf industry, and the production process belongs to the technical field of the production processes of the microbial preparations. The invention adopts the technical scheme as follows: using a high-temperature bacterial strain separated by the method of performing high-temperature separation on surface microbes on the tobacco leaves through a pure tobacco leaf leaching solution solid flat plate, inoculating the high-temperature bacterial strain into a fermentation culture medium which is cooled down to 60 DEG C after performing sterilization at the temperature of 121-126 DEG C and the pressure of 0.11-0.13MPa for 25-35 minutes, containing 8 plus or minus 5% (by weight / volume) of the waste tobacco leaves after smashing treatment and 92 plus or minus 95% of tap water, do not adjusting pH value, stirring and fermenting for 48 hours at 120-130rpm, maintaining the fermentation temperature at 45-55 DEG C, adding 0.1% of manganese sulfate after filtration and bacteria removal 2-4 hours before the end of the fermentation, stirring continuously till the end of the fermentation, placing into a pot, directly sub-packaging in a container, and sealing, wherein fermentation fluid is the microbial preparation for baking the tobacco leaves. Most of the high-temperature bacillus preparation prepared by the process can form spores, and the microbial preparation can be preserved for a long time and is environment-friendly and pollution-free.

Description

technical field [0001] The invention provides a microbial preparation production process for tobacco leaf curing to improve the industrial usability and quality of tobacco leaves, and belongs to the technical field of microbial preparation production technology. Background technique [0002] The fermentation process of microorganisms usually includes strains, medium composition, fermentation process control and post-treatment process. At present, the deep liquid preparation process of almost all microbial preparations includes strain preparation, medium composition, fermentation process control process and post-treatment process. The post-treatment process inevitably brings expensive equipment investment, huge energy consumption and pollution sources formed by fermentation broth. [0003] At present, some studies have been carried out on the liquid fermentation of Bacillus subtilis in China. Hao Linhua et al. (2006) reported the liquid fermentation conditions of Bacillus su...

Claims

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Application Information

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IPC IPC(8): C12N1/20A24B15/20C12R1/07
Inventor 杨程潘杰王毅赖永红张家伟
Owner KUNMING GUIXUN TECH
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