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Preparation method of immobilized cold resistant nitrobacteria

A nitrifying bacteria, cold-resistant technology, applied in chemical instruments and methods, sustainable biological treatment, water/sludge/sewage treatment, etc., can solve the problems of long generation time, slow growth of nitrifying bacteria, low ammonia nitrogen removal efficiency, etc., to achieve generation Short time, high ammonia nitrogen removal efficiency, not easy to lose effect

Inactive Publication Date: 2011-05-25
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of slow growth, long generation time and low removal efficiency of ammonia nitrogen in sewage obtained by the existing method of nitrifying bacteria, and to provide a preparation method of immobilized cold-resistant nitrifying bacteria

Method used

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  • Preparation method of immobilized cold resistant nitrobacteria

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specific Embodiment approach 1

[0009] Specific embodiment one: the preparation method of immobilized cold-resistant nitrifying bacteria in this embodiment is as follows: 1. Low-temperature resistant nitrifying bacteria flora: putting the sludge in the secondary settling tank of the spring sewage treatment plant into a low-temperature biochemical chamber with a temperature of 14-16°C In the incubator, cultivate under the condition that the sludge concentration is 2.0-3.0g / L and the dissolved oxygen is 2-3mg / L. At the same time, the domestic sewage is fed once every 2-3 days, and the domestic sewage is settled for 25 minutes before the domestic sewage is replaced. ~35 minutes, then pour out 2 / 3 of the supernatant, and then add the same amount of domestic sewage as the poured out supernatant, pass through domestic sewage for 2 to 3 times, replace part of the domestic sewage with nitrifying bacteria acclimation medium and continue Cultivate for 20-26 days, then lower the temperature of the low-temperature bioche...

specific Embodiment approach 2

[0015] Specific embodiment two: what this embodiment is different from specific embodiment one is: in the step 1, the nitrifying bacteria acclimation culture medium is made of 2.0g (NH 4 ) 2 SO 4 , 0.25g of NaH 2 PO 4 , 0.01g of MnSO 4 4H 2 O, 0.75 g of K 2 HPO 4 , 0.03g of MgSO 4 ·7H 2 O, 0.5g of CaCO 3 , 2.30g of Na 2 CO 3 and 1L of H 2 O composition. Other steps and parameters are the same as those in the first embodiment.

specific Embodiment approach 3

[0016] Specific embodiment three: this embodiment is different from one of the specific embodiments one to two: the nitrifying bacteria liquid culture medium in the step 2 is made of 2.0g (NH 4 ) 2 SO 4 , 0.25g of NaH 2 PO 4 , 0.01g of MnSO 4 4H 2 O, 0.75 g of K 2 HPO 4 , 0.03g of MgSO 4 ·7H 2 O, 0.5g of CaCO 3 , 2.30g of Na 2 CO 3 and 1L of H 2 O composition. Other steps and parameters are the same as those in the specific embodiment 1 to 2.

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Abstract

The invention provides a preparation method of immobilized cold resistant nitrobacteria, relating to a preparation method of cold resistant nitrobacteria. The invention aims at solving the problems that the nitrobacteria obtained by the existing method slowly grows, generation time is long, and removal efficiency of ammonia nitrogen in sewage is low. The preparation method comprises the steps of: 1, preparing low-temperature resistant nitrobacteria floras; 2, preparing cold resistant nitrobacteria floras; and 3, immobilizing the cold resistant nitrobacteria floras to obtain the immobilized cold resistant nitrobacteria. The nitrobacteria obtained by the method have the advantages of stable growth on condition of low temperature, short generation time and high removing rate of the ammonia nitrogen in the sewage.

Description

technical field [0001] The invention relates to a preparation method of cold-resistant nitrifying bacteria. Background technique [0002] Most nitrifying bacteria are chemoautotrophic bacteria, which grow slowly, and the average time is more than 10 hours. Temperature is an important factor affecting the growth of nitrifying bacteria. The suitable temperature for nitrification reaction is 20-30°C. When it is below 15°C, nitrification The speed drops, and below 10°C, the nitrification will be significantly weakened, and it will stop completely at 5°C. Since the average water temperature in winter in most parts of northern my country is around 10°C, the temperature is low, and the activity of nitrifying bacteria is low, making it difficult to meet the ammonia nitrogen standard in winter sewage treatment effluent. [0003] At present, it has been reported that pure strains of nitrifying bacteria have been isolated from low-temperature environments, but it is difficult to maint...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F3/02
CPCY02W10/10
Inventor 赫俊国金羽姜涛刘剑王岩
Owner HARBIN INST OF TECH
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