Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Earthworm protein-containing buccal instant preparation, preparation method and application thereof

A ground dragon protein, instant technology, applied in peptide/protein components, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve problems such as lower patient compliance

Inactive Publication Date: 2011-05-25
SHANGHAI UNIV OF T C M
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the preparation methods of oral protein pharmaceutical preparations are mostly freeze-dried, and there are many problems: how to ensure stability, how to eliminate the bad taste and reduce the patient's compliance, so as to prepare an oral protein that does not affect its activity and is easy to be accepted by patients. protein preparation
There is currently no information reported

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Earthworm protein-containing buccal instant preparation, preparation method and application thereof
  • Earthworm protein-containing buccal instant preparation, preparation method and application thereof
  • Earthworm protein-containing buccal instant preparation, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Embodiment 1: Preparation of earthworm active protein

[0077] Take fresh earthworms and wash them repeatedly in water, drain their digestive tract dirt, wash them, weigh them, add 2 times the amount of 20mM phosphate buffer with a pH value of 6.8, and mash them in a tissue grinder to make Homogenize, extract at room temperature for 4-6 hours, centrifuge at 4°C for 20 minutes at high speed, take the supernatant, slowly add the ground ammonium sulfate solid at 4°C, and stir while adding, so that the saturation of ammonium sulfate reaches 80 %, place overnight at 4°C, centrifuge at 4°C for 10 minutes at high speed, take the precipitate, suspend the precipitate with 20mM phosphate buffer saline with a pH value of 6.8, and put it in a dialysis bag with a molecular weight of 3500 for 20mM phosphate buffer with a pH value of 6.8 Dialyze for 48 hours, change the medium 4-5 times in the middle, centrifuge the obtained protein solution at 4°C for 20 minutes at high speed, and th...

Embodiment 2

[0078] Example 2: Preparation of Dilong Active Protein Oral Containing Instant Preparation

[0079] Add 5‰ aspartame, 10% pineapple essence, and 10% deodorizing essence to earthworm active protein, stir evenly, and freeze-dry according to the following steps:

[0080] Step 1: The heating rate is 2.1°C / min, the sample temperature is -30°C, and the freeze-drying time is 0.5 hours;

[0081] Step 2: The heating rate is 1.16°C / min, the sample temperature is 0°C, and the freeze-drying time is 6.0 hours;

[0082] Step 3: The heating rate is 1.01°C / min, the sample temperature is 5°C, and the freeze-drying time is 10.0 hours;

[0083] Step 4: The heating rate is 1.16°C / min, the sample temperature is 20°C, and the freeze-drying time is 20.0 hours.

Embodiment 3

[0084] Example 3: Research on the antiasthmatic effect of earthworm active protein

[0085] 1. Modeling method

[0086] Ovalbumin (III grade) was dissolved in physiological saline to prepare 5% ovalbumin physiological saline solution. Each guinea pig was injected intramuscularly with 0.5 ml and intraperitoneally with 1 ml in both hind legs. After 2 weeks, the sensitized guinea pig was placed in an airtight glass bell jar and stimulated with 0.1% ovalbumin atomization.

[0087] 2. Grouping and dosage

[0088] 40 guinea pigs, half ♀♂, were randomly divided into 5 groups, 8 in each group, the five groups were: blank group, positive drug (montelukast sodium tablets) control group, high, middle and low administration concentration group. The concentration is set to be 0.12g / kg of fresh Dilong antiasthma active protein as the middle concentration, and then rise and fall to 0.06g / kg and 0.24g / kg respectively. The dosage was set at 10ml / kg. The preparation of protein solutions wi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an animal protein medicament buccal instant preparation, and particularly discloses an earthworm protein-containing buccal instant preparation and a preparation method thereof. The preparation consists of 75 weight percent of earthworm protein extract and 25 weight percent of composite flavoring agent, wherein the protein content of the earthworm protein extract is more than 50 percent. In addition, the invention also discloses the preparation method and application of the preparation. The preparation has the obvious antiasthmatic effect, and can effectively inhibit asthma reaction caused by antigen attack of eggalbumin sensitized guinea pigs; and the action mechanism of the preparation is possibly related to anti-leukotriene effect through preliminary analysis of isolated trachea smooth muscle.

Description

technical field [0001] The invention relates to an oral instant preparation of animal protein drugs and a preparation method thereof, in particular to an oral instant preparation of earthworm protein and a preparation method thereof. In addition, the present invention also relates to the application of the preparation. Background technique [0002] The name of the earthworm medicine is Dilong, and its medicinal use is first recorded in "The Book of Songs", and "Shen Nong's Herbal Classic" is listed as a lower grade. The traditional Chinese medicine Dilong has the effects of clearing away heat and calming convulsions, dredging collaterals, relieving asthma and diuresis, and is a commonly used animal medicinal material. The earthworms recorded in the Chinese Pharmacopoeia 2005 edition are Pheretima aspergillum (E.Perrier), Pheretimavulagris Chen, Pheretima guillelm (Michaelsen) or Pheretimaectinifera Michaelsen. dry body. In addition to the species recorded in the Pharmacop...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K35/64A61K38/17A61P11/06A61K35/62
Inventor 冯怡徐德生阮克锋
Owner SHANGHAI UNIV OF T C M
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com