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Method for preprocessing and identifying electron transfer dissociation (ETD) mass spectrum

An electron transport and preprocessing technology, applied in the field of protein identification, can solve the problems of poor accuracy and low efficiency of ETD mass spectrometry data analysis, and achieve the effect of avoiding interference and improving the identification effect.

Active Publication Date: 2011-05-04
INST OF COMPUTING TECH CHINESE ACAD OF SCI +1
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  • Abstract
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Problems solved by technology

[0009] The purpose of the present invention is to overcome the defects of low efficiency and poor accuracy of ETD mass spectrometry data analysis in existing methods, thereby providing an efficient and accurate ETD mass spectrometry data analysis and identification method

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  • Method for preprocessing and identifying electron transfer dissociation (ETD) mass spectrum
  • Method for preprocessing and identifying electron transfer dissociation (ETD) mass spectrum
  • Method for preprocessing and identifying electron transfer dissociation (ETD) mass spectrum

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Embodiment Construction

[0051] The present invention will be described below in conjunction with the accompanying drawings and specific embodiments.

[0052] Before the present invention is described in detail, in order to facilitate understanding, the meanings of several technical terms involved in the present invention are explained separately.

[0053] Precursor ion: Refers to fragmented peptide ions selected from the primary spectrum.

[0054] Derived peak: refers to the spectrum peak in which the protonated polypeptide ion interacts with electrons, and the mass is basically unchanged but the charge is reduced.

[0055] Neutral loss peak: refers to the spectral peak generated by the neutral loss of the side chain of the derivative peak.

[0056] After explaining some technical terms involved in the present invention, the ETD experimental tandem mass spectrum generated by the ETD method is analyzed below in conjunction with examples.

[0057] figure 1 An ETD spectrum of the polypeptide AVYAGENF...

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Abstract

The invention provides a method for preprocessing an electron transfer dissociation (ETD) mass spectrum, comprising the steps of: calculating a mass to charge ratio and a charge state of a parent ion; calculating the possible areas of a parent ion peak and isotopic peaks thereof in the ETD mass spectrum; calculating the possible areas of a series of derivative peaks of the parent ion in the ETD mass spectrum; calculating the possible areas of neutral lost peaks of the parent ion in the ETD mass spectrum; and removing the spectrum peaks in the calculated areas in the ETD mass spectrum so as to remove the non-fragmented parent ion peaks, the series of derivative peaks of the parent ion and the neutral lost peaks of the parent ion.

Description

technical field [0001] The invention relates to the field of protein identification, in particular to a pretreatment method and an identification method for electron transport cleavage mass spectrometry. Background technique [0002] In molecular biology, the dissemination of information follows the "central dogma", that is, DNA generates messenger RNA through the process of transcription, and messenger RNA generates protein through the process of translation. In the process of protein translation from RNA, the chain molecules formed by connecting 20 kinds of amino acids in sequence with peptide bonds are called peptides, and the peptides whose molecular weight reaches a certain level are called proteins. There are many types of proteins, and the process of identifying a specific type of protein is called protein identification. The method for identifying proteins in the prior art is mainly to compare the experimental tandem mass spectrum generated from the protein sample t...

Claims

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Application Information

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IPC IPC(8): G01N27/62
Inventor 孙瑞祥董梦秋宋春青迟浩杨兵王乐珩付岩贺思敏
Owner INST OF COMPUTING TECH CHINESE ACAD OF SCI
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