Method for extracting L-isoleucine
A technology of isoleucine and strong acid cation, applied in the field of extracting L-isoleucine to achieve the effects of reducing dosage, protecting natural environment, and improving extraction rate and quality
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Embodiment 1
[0036] Embodiment 1: The method of the present invention extracts L-isoleucine
[0037] 1. Extraction method
[0038] For the enzyme-inactivated L-isoleucine fermentation broth, adjust the pH of the fermentation broth to 3 with hydrochloric acid, add chitosan in an amount of 0.1%, maintain the flocculation temperature at 20° C., and stir for 30 minutes. The pore size is 50nm, the molecular weight is 30KD, and the area is 117m 2 The ceramic membrane is used for microfiltration of the clarified solution after flocculation, the operating pressure of the ceramic membrane is controlled to be 0.2Mpa, the temperature of the membrane is 40°C, the amount of dialyzed water added is 30% (volume ratio) of the fermentation broth, and the filtrate is mixed with L-isoleucine The concentration ratio of the fermentation broth is 1:6. The filtrate is directly adsorbed on the 732-type strong acid cationic resin, and the column loading speed is 0.2m / s. After the adsorption is saturated, the re...
Embodiment 2
[0044] Embodiment 2: The method of the present invention extracts L-isoleucine
[0045] 1. Extraction method
[0046] For the enzyme-inactivated L-isoleucine fermentation broth, adjust the pH of the fermentation broth to 3 with hydrochloric acid, add aluminum chloride in an amount of 4%, maintain the flocculation temperature at 70°C, and stir for 120 minutes. The pore size is 50nm, the molecular weight is 30KD, and the area is 117m 2 The ceramic membrane is used for microfiltration of the clarified solution after flocculation, the operating pressure of the ceramic membrane is controlled to be 0.8Mpa, the temperature of the membrane is 60°C, the amount of dialyzed water added is 50% (volume ratio) of the fermentation broth, and the filtrate is mixed with L-isoleucine The concentration ratio of the fermentation broth is 1:10. The filtrate is directly adsorbed on the JK008 type strong acid cationic resin, and the column loading speed is 1m / s. After the adsorption is saturated,...
Embodiment 3
[0052] Embodiment 3: The method of the present invention extracts L-isoleucine
[0053] 1. Extraction method
[0054] For the enzyme-inactivated L-isoleucine fermentation broth, adjust the pH of the fermentation broth to 3 with hydrochloric acid, add aluminum chloride in an amount of 4%, maintain the flocculation temperature at 45°C, and stir for 60 minutes. The pore size is 50nm, the molecular weight is 30KD, and the area is 117m 2 The ceramic membrane is used for microfiltration of the clarified solution after flocculation, the operating pressure of the ceramic membrane is controlled to be 0.5Mpa, the temperature of the membrane is 50°C, the amount of dialyzed water added is 40% (volume ratio) of the fermentation broth, and the filtrate is mixed with L-isoleucine The concentration ratio of the fermentation broth is 1:8. The filtrate is directly adsorbed on the JK006 type strong acid cationic resin, and the column loading speed is 0.6m / s. After the adsorption is saturated,...
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