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Method for detecting phytase activity in feed

A technology of phytase activity and phytase, which is applied in the field of chemical composition analysis of feed, can solve the problems of many detection steps, long cycle, no background interference and impurity removal, etc., and achieve the effect of good repeatability and accuracy

Active Publication Date: 2011-04-27
WUHAN SUNHY BIOLOGICAL
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Problems solved by technology

However, the determination of phytase activity in feed has the following problems: phytase in feed is different from pure phytase, and there are a large number of substances in it that can react with ammonium vanadium molybdate under acidic conditions and interfere with the 415nm Colorimetric Response at Wavelength
GB / T 18634-2009 does not establish a background interference removal step; at the same time, although Chinese patent CN101493418A (a micro-measurement method of phytase added in feed, published on July 29, 2009) adopts the method of dialysis The background interference is removed, but the molecular weight cut-off of the dialysis bag is not indicated, and the detection method disclosed in this document involves dialysis treatment, with many detection steps and a long cycle

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  • Method for detecting phytase activity in feed
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  • Method for detecting phytase activity in feed

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[0026] The following are specific examples of the present invention, and further describe the technical solution of the present invention, but the protection scope of the present invention is not limited to these examples. All changes or equivalent substitutions that do not depart from the concept of the present invention are included in the protection scope of the present invention.

[0027] 1 Reagents and solutions

[0028] Unless otherwise specified, the reagents used are of analytical grade, and the water is the secondary water specified in GB / T 6682. Do not use phosphorus-containing cleaning agents to clean experimental containers.

[0029] 1.1 Extraction solution: Weigh 46.06g potassium oxalate monohydrate and 9.8g potassium acetate into a 1000ml volumetric flask, add 950ml water to dissolve, adjust the pH value to 5.50±0.01 with glacial acetic acid, add 0.25g bovine serum albumin to dissolve, and use Make up to 1000ml with distilled water and store at room temperature...

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Abstract

The invention relates to a method for detecting phytase activity in a feed and belongs to the technical field of chemical composition analysis of feeds. In the method, phytase in the feed is completely leached through novel extract and diluent and a detection flow, the interference of inorganic phosphorus present in the feed on the detection is eliminated, the coefficient of variation of the detected result is small, and the method has the advantages of short detection period, high sensitivity, high repeatability and high accuracy.

Description

technical field [0001] The invention relates to a method for detecting phytase activity in feed, belonging to the technical field of chemical composition analysis of feed. Background technique [0002] Phytase is a biological macromolecular protein substance. In the pure state, phytase can hydrolyze the substrate sodium phytate under certain temperature and pH conditions to generate orthophosphoric acid and inositol derivatives. Medium and vanadium ammonium molybdate form a yellow complex, and the colorimetric determination is carried out at a wavelength of 415nm. At present, the national standard GB / T 18634-2009 "Spectrophotometric method for the determination of feed phytase activity" has been formed. However, the determination of phytase activity in feed has the following problems: phytase in feed is different from pure phytase, and there are a large number of substances in it that can react with ammonium vanadium molybdate under acidic conditions and interfere with the ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 邹大琼詹志春
Owner WUHAN SUNHY BIOLOGICAL
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